Vol 54, No 3 (2009)
- Year: 2009
- Published: 15.06.2009
- Articles: 9
- URL: https://virusjour.crie.ru/jour/issue/view/76
Human bocavirus
Abstract
Human bocavirus (HBoV) is a newly identified parvovirus associated with acute respiratory infections in young children in different parts of the world. It is not inconceivable that this virus is also capable of causing acute gastroenteritis and asymptomatically persisting in infected children. HBoV is the third widespread human respiratory virus after respiratory syncytial virus and rhinovirus. Polymerase chain reaction remains the most reliable of HBoV detection in clinical samples. Phylogenetic analysis shows the presence of at least 2 circulating variants (genotypes) of HBoV.
Problems of Virology. 2009;54(3):4-7
4-7
Immunodeficiency viruses and nonhuman primate models of AIDS
Abstract
Monkeys as the phylogenetically closed animals to human beings are widely used for simulating many human diseases. Moreover, nonhuman primate models of AIDS take a special place and vaccine testing without their participation is simply inconceivable. The article reviews the data available in the literature on immunodeficiency viruses and the basic nonhuman models of AIDS.
Problems of Virology. 2009;54(3):8-11
8-11
Autoimmune response in HIV-infected patients is directed against CD4 domain 4
Abstract
Phage display epitope library technology and a novel computer algorithm have been used for the localization of CD4 epitopes specific for monoclonal antibody (mAb) T6 and autoimmune antibodies found in an HIV infected patient. Both predicted epitope clusters have been shown to overlap and to be localized within the domain 4 of CD4. They included Cys303, Glu304, Glu330, and Lys331 amino acids. Few amino acids predicted by the algorithm as the epitope residues and two residues that did not relate to the epitope were sequentially substituted for Ala. Further analysis of the mutated forms of sCD4 expressed in 293T cells transfected with the corresponding DNAs, supported the predicted localization of the mAb T6 epitope. The results demonstrate that the autoimmune response in HIV-infected patients is directed against domain D4 of sCD4.
Problems of Virology. 2009;54(3):12-16
12-16
Prediction of phenotype R5/X4 of HIV-1 variants circulating in Russia, by using computer methods
Abstract
The paper presents the data of a comparative analysis of various bioinformation methods for evaluating the tropism of HIV-1 subtype A that is typical of Russia. The prevalence of CXCR4- and CCR5-tropic viruses among the variants prevailing in Russia was studied using the B-PSSM (sinsi) prediction method. No X4-tropic variants were found among 124 samples of HIV-1 isolated from the Russian patients not receiving antiretrovirus therapy.
Problems of Virology. 2009;54(3):17-20
17-20
Interaction of influenza A and B viruses with polyaniline nano complexes
Abstract
The paper gives data on the sorption of influenza A and B viruses diluted from different solutions on polyaniline sorbents synthesized with low and high molecular-weight polymer acids. Irrespective of the antigenic structure of surface proteins, the influenza A and B viruses isolated in the period of 1977 to 2008 were shown to be able to be immobilized on polyaniline sorbents in the temperature range of 4 to 37°C. The immobilized viruses interacted with homologous immune sera.
Problems of Virology. 2009;54(3):21-25
21-25
Preclinical and clinical studies of the efficacy of panavir in therapy for tick-borne encephalitis
Abstract
The antiviral activity of Panavir was studied on a model of mice infected with the strain Sofjin of tick-borne encephalitis (TBE) virus. The efficacy of Panavir was studied in the treatment of the chronic (monotherapy; 30 patients) and acute febrile and meningeal (combined therapy; n = 113) forms of TBE. Panavir was found to have a positive effect on the clinical course of TBE and the immune mechanisms of the body's protection.
Problems of Virology. 2009;54(3):26-32
26-32
The genetic variability and genotyping of tick-borne encephalitis virus with deoxyoligonucleotide probes
Abstract
A panel of genotype-specific molecular probes has been designed, which is used to indicate and differentiate tick-borne encephalitis (TBE) virus. It assesses the individual genetic structure of each strain since the targets for the probes are the variable sequences of all 10 virus genes, which are specific for each of three genotypes. The molecular nucleic acid hybridization by means of the panel was used to study 273 TBE virus strains isolated from a Eurasian area; along with the representatives of three genotypes, the virus strains, the genomic structures of which do not fit in the established concept on three genotypes, circulate in Eastern Siberia.
Problems of Virology. 2009;54(3):33-41
33-41
Effect of Stimforte in combination with Virazole on infection induced by hepatitis C virus in mice
Abstract
Twenty-four hours after intramuscular injection of Stimforte in a dose of 25 mg in mice weighing 18-20 g, chronically infected with hepatitis C virus (HCV), viral infection was shown to be at the most suppressed viral replication, as suggested by the data of the infectious and antigenic activities of HCV. Following 72 hours of its administration, the quantity of viral antigen and the infectious activity restored.
Readministration of the agent considerably suppressed HCV replication. When given in a dose of 12.5 mg/kg, the agent reduced HCV titers by 2.0-2.5 log10 TCID50; when used in a dose of 25 mg/kg, it diminished the infectious activity of HCV by 3.2 log. The similar data were obtained in the study of the antigenic activity of HCV in infected animals. The effect of Virazole in combination with Stimforte in reducing the replication of infectious HCV and the accumulation of antigens in HCV-infected mice was additive or synergic, suggesting that it is expedient to use them concurrently.
Problems of Virology. 2009;54(3):42-44
42-44
Antigenic activity of human herpes virus type 8 proteins coding orfK8.1 and orf65
Abstract
peptide scanning of the sequences of human herpes virus type 8 (HHV-8) proteins coding orfK8.1 and orf65 was made. The antigenic activity of peptides was evaluated by ELISA. The fragment 101-213 aa of orfK8.1-encoded protein was shown to consist of two sites containing linear B-epitopes that had a weak antigenic activity: 121-145 and 177-209 aa. The protein coding orf65 comprised 2 sites at which linear B-cell epitopes 78-110 aa (a weak antigenic activity) and 142-170 aa (a strong antigenic activity) were located.
Problems of Virology. 2009;54(3):45-48
45-48
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