Email this article Development and evaluation of the RT-PCR kit for the rabies virus diagnosis
- Authors: Dedkov V.G.1,2, Deviatkin A.A.1,2,3, Poleschuk E.M.4, Safonova M.V.1,3, Markelov M.L.2, Shipulin G.A.1
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Affiliations:
- Central Research Institute for Epidemiology
- Institute of Occupational Health
- Chumakov Institute of Poliomyelitis and Viral Encephalitides
- Omsk Research Institute of Natural Foci Infections
- Issue: Vol 61, No 5 (2016)
- Pages: 235-240
- Section: TO VIROLOGIST’S AID
- Submitted: 20.01.2020
- Published: 28.10.2016
- URL: https://virusjour.crie.ru/jour/article/view/94
- DOI: https://doi.org/10.18821/0507-4088-2016-61-4-235-240
- ID: 94
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Abstract
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*103 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
Keywords
About the authors
V. G. Dedkov
Central Research Institute for Epidemiology; Institute of Occupational Health
Author for correspondence.
Email: vgdedkov@yandex.ru
Россия
A. A. Deviatkin
Central Research Institute for Epidemiology; Institute of Occupational Health; Chumakov Institute of Poliomyelitis and Viral Encephalitides
Email: noemail@neicon.ru
Россия
E. M. Poleschuk
Omsk Research Institute of Natural Foci Infections
Email: noemail@neicon.ru
Россия
M. V. Safonova
Central Research Institute for Epidemiology; Chumakov Institute of Poliomyelitis and Viral Encephalitides
Email: noemail@neicon.ru
Россия
M. L. Markelov
Institute of Occupational Health
Email: noemail@neicon.ru
Россия
G. A. Shipulin
Central Research Institute for Epidemiology
Email: noemail@neicon.ru
Россия
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).