PCR study of the human herpes virus type 6 and other viruses of the herpes group in eye diseases

Cover Page

Cite item

Full Text

Abstract

To study the role of the HHV-6 type in the development of eye diseases PCR tests of blood (152), cornea biopsies (61), and intraocular fluids (11) for HHV-6 and other viruses of the herpes group (HSV type 1 and 2, CMV, EBV) were conducted. It was found that the HHV-6, along with other representatives of the Herpesviridae, can be detected in patients with different clinical forms of ophthalmopathology (174 patients were surveyed). Viral DNA was detected in blood, cornea, and in the anterior chamber fluid. The obtained data allow that the HHV-6 to be suggested as a possible cause of the ophthalmic herpes along with the other viruses of this group. It makes finding the virus DNA an essential step towards setting the etiologic diagnosis of the ophthalmological patients.

About the authors

O. S. Slepowa

Moscow Helmholtz Research Institute of Eye Diseases

Author for correspondence.
Email: slepowaolga@yandex.ru

Ol’ga Slepova, , MD,PhD, ScD, prof.

105062, Moscow

Russian Federation

E. V. Svetlova

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

L. A. Kovaleva

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

P. V. Makarov

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

A. E. Kugusheva

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

E. V. Denisova

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

E. S. Vahova

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

G. Yu. Zaharova

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

Yu. A. Kondrat’eva

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

A. E. Andryushin

Moscow Helmholtz Research Institute of Eye Diseases

Email: fake@neicon.ru
105062, Moscow Russian Federation

V. V. Demkin

Institute of Molecular Genetics, Russian Academy of Sciences

Email: fake@neicon.ru
123182, Moscow Russian Federation

References

  1. Каспаров А.А. Офтальмогерпес. М.: Медицина; 1994.
  2. Майчук Ю.Ф. Герпесвирусные заболевания глаз. В кн.: Неизвестная эпидемия: герпес. Смоленск; 1997: 62–74.
  3. Кускова Т.К., Белова Е.Г. Семейство герпесвирусов на современном этапе. Лечащий врач. 2004; 5: 3–6.
  4. Кричевская Г.И., Анджелов В.О., Катаргина Л.А., Старикова А.В., Денисова Е.В., Звонарев А.Ю. и др. Реактивация персистентных герпесвирусных инфекций как фактор патогенеза эндогенных увеитов у детей. Вестник офтальмологии. 2005; 121 (2): 22–4.
  5. Шевчук Н.Е., Мальханов В.Б., Матюхина Е.Н. Диагностика и лечение цитомегаловирусного хориоретинита. В кн.: Материалы научно-практической конференции Восток-Запад 2012. Уфа: ДизайнПресс; 2012: 360.
  6. Fillet A.M., Reux I., Joberty C., Fournier J.G., Hauw J.J., Le Hoang P. et al. Detection of human herpes virus 6 in AIDS-associated retinitis by means of in situ hybridization, polymerase chain reaction and immunohistochemistry. J. Med. Virol. 1996; 49:289–95.
  7. Mitchell S.M., Fox J.D., Tedder R.S., Gazzard B.G., Lightman S. Vitreous fluid sampling and viral genome detection for the diagnosis of viral retinitis in patients with AIDS. J. Med. Virol. 1994; 4: 336–40.
  8. Слепова О.С., Быковская Г.Н., Макаров П.В., Балаян Т.Г., Оганесян О.Г., Илуридзе С.Л. Исследование эффективности применения циклоспорина у больных с высоким риском отторжения кератотрансплантата. Сообщение 2. Результаты иммунологического исследования. Вестник офтальмологи . 2007; 4:19–22.
  9. Миронкова Е.А., Демкин В.В., Слепова О.С., Садохина Т.С., Макаров П.В., Кугушева А.Э. Диагностика и роль ВГЧ-6 инфекции при кератопластике высокого риска. Российский офтальмологический журнал. 2012; 3: 30–4.
  10. Миронкова Е.А., Макаров П.В., Слепова О.С., Гундорова Р.А., Кугушева А.Э., Демкин В.В. и др. Инфицированность донорского материала вирусами группы герпеса как возможная причина развития болезни трансплантата при сквозной кератопластике. Вестник трансплантологии и искусственных органов. 2012; 4: 48–51.
  11. Barone S.R., Kaplan M.H., Krilov L.R. Human herpesvirus-6 infection in children with first febrile seizures. J. Pediatr. 1995; 127 (1): 95–7.
  12. Amirian E.S., Adler-Storthz K., Scheurer M.E. Associations between human herpesvirus-6, human papillomavirus and cervical cancer. Cancer Lett. 2013; 336 (1): 18–23.
  13. de Boer J.H., Verhagen C., Bruinenberg M., Rothova A., de Jong P.T., Baarsma G.S. et al. Serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. Am. J. Ophthalmol. 1996; 121 (6): 650–8.
  14. de Groot-Mijnes J.D., de Visser L., Zuurveen S., Martinus R.A., Völker R., ten Dam-van Loon N.H. et al. Identification of new pathogens in the intraocular fluid of patients with uveitis. Am. J. Ophthalmol. 2010; 150 (5): 628–36.
  15. Maslin J., Bigaillon C., Froussard F., Enouf V., Nicand E. Acute bilateral uveitis associated with an active human herpesvirus-6 infection. J. Infect. 2007; 54 (4): 237–40.
  16. Okuno T., Hooper L.C., Ursea R., Smith J., Nussenblatt R., Hooks J.J. et al. Role of human herpes virus 6 in corneal inflammation alone or with human herpesviruses. Cornea. 2011; 30 (2): 204–7.
  17. Mechai F., Boutolleau D., Manceron V., Gasnault J., Quertainmont Y., Brosseau J.P. et al. Human herpesvirus 6–associated retrobulbar optic neuritis in an HIV-infected patient: response to anti-herpesvirus therapy and long-term outcome. J. Med. Virol. 2007; 79 (7): 931–4.
  18. Moschettini D., Franceschini R., Vaccaro N.M., Cermelli C., Pezzini F., Balestrieri M. et al. Human herpesvirus-6B active infection associated with relapsing bilateral anterior optic neuritis. J. Clin. Virol. 2006; 37 (4): 244–7.
  19. Oberacher-Velten I.M., Jonas J.B., Jünemann A., Schmidt B. Bilateral optic neuropathy and unilateral tonic pupil associated with acute human herpesvirus 6 infection: a case report. Graefes Arch. Clin. Exp. Ophthalmol. 2005; 243 (2): 175–7.
  20. Sugita S., Shimizu N., Watanabe K., Ogawa M., Maruyama K., Usui N. et al. Virological analysis in patients with human herpes virus 6-associated ocular inflammatory disorders. Invest. Ophthalmol. Vis. Sci. 2012; 53 (8): 4692–8.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Slepowa O.S., Svetlova E.V., Kovaleva L.A., Makarov P.V., Kugusheva A.E., Denisova E.V., Vahova E.S., Zaharova G.Y., Kondrat’eva Y.A., Andryushin A.E., Demkin V.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies