Multiplex immunoassay for detection of immunoglobulin G to herpes simplex virus types 1, 2 and cytomegalovirus based on PHOSPHAN technology

Cover Page


Cite item

Full Text

Abstract

We have developed a multiplex immunoassay test (immunochip) based on PHOSPHAN technology for the detection of immunoglobulin G to herpes simplex virus (HSV) types 1, 2 and cytomegalovirus (CMV). The immunochip consists of HSV type specific gG1 (HSV-1) and gG2 (HSV-2) recombinant antigens, the lysate antigen for detection of total IgG to both HSV types (HSV 1/2), and CMV specific chimeric recombinant antigen containing the immunodominant sequences of pp150, gB, pp28 and pp52 proteins. The sensitivity and specificity of simultaneous IgGs detection with recombinant proteins were comparable to the commercial ELISA kits regardless of the kind of investigated serum specimens (patient sera, standard serum panels). The lysate HSV antigen was as sensitive but significantly less specific, so that it could not be recommended for use as a component of the multiplex test. These results can be used as a basis for creating commercial multiplex tests intended for high-productive screening of HSV, CMV and other TORCH-infections in a clinical laboratory.

About the authors

A. V. Nikitina

State Research Institute of Biological Engineering

Author for correspondence.
Email: an-na-nikitina@ya.ru
Russian Federation

V. G. Pomelova

State Research Institute of Biological Engineering

Email: noemail@neicon.ru
Russian Federation

N. S. Osin

Immunoscreen, Closed Joint Stock Company

Email: noemail@neicon.ru
Russian Federation

S. G. Mardanly

Ekolab, Closed Joint Stock Company

Email: noemail@neicon.ru
Russian Federation

References

  1. Brown Z.A. HSV-2 specific serology should be offered routinely to antenatal patients. Rev. Med. Virol. 2000; 10 (3): 141-4.
  2. Newton E.R. Diagnosis of perinatal TORCH infections. Clin. Obstet. Gynecol. 1999; 42 (1): 59-70.
  3. Benedetti J.K., Corey L., Asley R. Recurrence rates in genital herpes after symptomatic first-episode infection. Ann. Intern. Med. 1994; 121 (11): 847-54.
  4. Koutsky L.A., Ashley R.I., Holmes K.K., Stevens C.E., Wolner-Hanssen P., Critchlow C.W. et al. The frequency of unrecognized type 2 herpes simplex virus infection among women: implications for the control of genital herpes. Sex. Transm. Dis. 1990; 17 (2): 90-4.
  5. Sucato G., Wald A., Wakabayashi E., Vieira J., Corey L. Evidence of latency and reactivation of both herpes simplex virus (HSV-1) and HSV-2 in the genital region. J. Infect. Dis. 1998; 177 (4): 1069-72.
  6. Kit S., Trkula D, Qavi H., Dreesman G., Kennedy R.S., Adler-Storthz K. et al. Sequential genital infections by herpes simplex viruses types 1 and 2: restriction nuclease analyses of viruses from recurrent infections. Sex. Transm. Dis. 1983; 10 (2): 67-71.
  7. al Samarai A.M., Shareef A.A., Kinghorn G.R., Potter C.W. Sequential genital infections with herpes simplex virus types 1 and 2. Genitourin Med. 1989; 65 (1): 39-41.
  8. Brown Z.A., Benedetti J., Ashley R., Burchett S., Selke S., Berry S. et al. Neonatal herpes simplex virus infection in relation to asymptomatic maternal infection at the time of labor. N. Engl. J. Med. 1991; 324 (18): 1247-52.
  9. Brown Z.A., Selke S., Zeh J., Kopelman J., Maslow A., Ashley R.L. et al. Acquisition of herpes simplex virus during pregnancy. N. Engl. J. Med. 1997; 337 (8): 509-15.
  10. Whitley R.J., Roizman B. Herpes simplex virus infections. Lancet. 2001; 357 (9267): 1513-8.
  11. McGeoch D.J., Moss H.W., McNab D., Frame M.C. DNA sequence and genetic content of the Hindlll I region in the short unique component of the herpes simplex virus type 2 genome: identification of the gene encoding glycoprotein G, and evolutionary comparisons. J. Gen. Virol. 1987; 68 (Pt.1): 19-38.
  12. Tunbäck P., Bergström T., Löwhagen G.B., Hoebeke J., Liljeqvist J.A. Type-specific reactivity of anti-glycoprotein G antibodies from the herpes simplex virus infected individuals is maintained by single or dual type-specific residues. J. Gen. Virol. 2005; 86 (Pt.2): 247-51.
  13. Jiang L., Yu Z., Tang Z., Jiang T., Zhang C., Lu Z. Protein arrays based on biotin-streptavidin system for the simultaneous detection of TORCH infections. J. Nanosci. Nanotechnol. 2008; 8 (5): 2286-92.
  14. Mezzasoma L., Bacarese-Hamilton T., Di Cristina M., Rossi R., Bistoni F., Crisanti A. Antigen microarrays for serodiagnosis of infectious diseases. Clin. Chem. 2002; 48 (1): 121-30.
  15. Wu D., Wu Y., Wang L., Xu W., Zhong Q. Evaluation of a novel array-based toxoplasma, rubella, cytomegalovirus, and herpes simplex virus IgG enzyme linked immunosorbent assay and its comparison with Virion/Serion enzyme linked immunosorbent assays. Ann. Lab. Med. 2014; 34 (1): 38-42.
  16. Osin N.S., Pomelova V.G. Multi-array immunophosphorescence technology for the detection of pathogens. In: Georgiev V.S., Western K.A., McGowan J.J., eds. Frontiers in Research. National Institute of Allergy and Infectious Diseases, NIH. Totowa, NJ: Humana Press; 2008.
  17. Pomelova V.G., Korenberg E.I., Kuznetsova T.I., Bychenkova T.A., Bekman N.I., Osin N.S. C6 peptide-based multiplex Phosphorescence Analysis (PHOSPHAN) for serologic confirmation of Lyme Borreliosis. PLoS One. 2015; 10 (7): e0130048.
  18. Никитина А.В., Помелова В.Г., Соколова М.В., Осин Н.С., Марданлы С.Г. Выбор антигенов для определения иммуноглобулина G к цитомегаловирусу на основе технологии ФОСФАН™. Клиническая лабораторная диагностика. 2015; 60 (10): 36-9.
  19. Wald A., Ashley-Morrow R. Serological testing for herpes simplex virus (HSV)-1 and HSV-2 infection. Clin. Infect. Dis. 2002; 35 (Suppl. 2): 173-82.
  20. LeGoff J., Péré H., Bélec L. Diagnosis of genital herpes simplex virus infection in the clinical laboratory. Virol. J. 2014; 11: 83-99.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2017 Nikitina A.V., Pomelova V.G., Osin N.S., Mardanly S.G.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies