Email this article Chicken yolk IgY preparations as immune reagents to tick-borne encephalitis virus
- Issue: Vol 55, No 2 (2010)
- Pages: 46-48
- Section: Articles
- URL: https://virusjour.crie.ru/jour/article/view/12023
- ID: 12023
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Abstract
This paper provides the first (as is known from the literature analysis) results of the use of specific egg yolk IgY from immunized hens to prepare immune reagents to tick-borne encephalitis virus (TBEV). The IgY preparations are shown to possess a high specific activity in ELISA and can be used as reference reagents, and also for the construction of ELISA systems to detect TBEV antigen.
References
- Acita E. M., Nakai S. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain // J. Immunol. Meth. - 1993. - Vol. 160. - P. 207-214.
- Chao-Yang Fit, He Huang, Xiao-Mei Wang et al. Preparation and evaluation of antiSARS coronavirus IgY from yolks of inmiunized chickens // J. Virol. Meth. - 2006. - Vol. 133. - P. 112-115.
- Haak-Frendscho M. Why IgY? Chicken polyclonal antibody, an appealing alternative // Promega Notes Magazine. - 1994. - N 46. - P. 11-14.
- Ivanov A. P., Bashkirtsev V. N., Tkachenko E. A. Enzyme-linked immunosorbent assay for detection of arenaviruses // Arch. Virol. - 1981. - Vol. 67. - P. 71-74.
- Schade R., Staak C., Hendriksen C. et al. The production of avian (egg yolk) antibodies: IgY. The report and recommendations of ECVAM workshop 21 // ATLA. - 1997. - Vol. 24. - P. 925-934.
- Schade R., Calzado E. G., Sarmiento R. et al. Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine // ATLA. -2005. - Vol. 33. - P. 129-154.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).