Email this article Antiviral activity of human recombinant y-interferon and recombinant hybrid proteinof tumor necrosis factor-a-thymosin-сц on modelsof herpes-virus and cytomegalovirus infection invitro
- Issue: Vol 49, No 5 (2004)
- Pages: 37-40
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.10.2004
- URL: https://virusjour.crie.ru/jour/article/view/11877
- ID: 11877
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Abstract
The conducted studies showed a certain efficiency of gene-engineering preparation of IFN-y and TNF-T in virus
infections caused by herpes simplex virus 2 (HSV-2) and cytomegalovirus (CMV) in cell cultures of human embryo
fibroblast (HEF). The drugs have no viricidal action. IFN-y, when used according to the treatment scheme in vitro,
proved to be more effective versus TMF-T both in HSV-2 and in CMV. It inhibited significantly the HSV-2 reproduction
within the dilution range of 1: 50 to 1 : 500. It was also effective, when used in CMV, within the dilution range
of 1 : 5000 and lower, whereas TNF-T was effective within the range of 1 : 500 and lower as well as in 0.1 multiple
infection. A significantly higher effect was ensured when the drugs were used for prevention. In HSV-2, IFN-y inhibited
the virus reproduction, like in the treatment scheme, within the dilution range of 1 : 50 to 1 : 500, whereas
TNF-T was effective in the range of 1 : 50. In CMV, the drugs' effect, when used for prevention, was similar to that
observed in the treatment scheme. The highest inhibition values were registered for HSV-2, when it was used 24
hours before infection (IFN-y - 2.25 lg, dilution range of 1 : 50; TNF-2 -1.0 lg, dilution range of 1 : 50). IFN-y and
TNF-2 exert a synergic action on different stages of virus reproduction. A reliable additive effect was ensured in
prevention made 4 hour before infection by IFN-y and TNF-T only in experimental CMV infection.
infections caused by herpes simplex virus 2 (HSV-2) and cytomegalovirus (CMV) in cell cultures of human embryo
fibroblast (HEF). The drugs have no viricidal action. IFN-y, when used according to the treatment scheme in vitro,
proved to be more effective versus TMF-T both in HSV-2 and in CMV. It inhibited significantly the HSV-2 reproduction
within the dilution range of 1: 50 to 1 : 500. It was also effective, when used in CMV, within the dilution range
of 1 : 5000 and lower, whereas TNF-T was effective within the range of 1 : 500 and lower as well as in 0.1 multiple
infection. A significantly higher effect was ensured when the drugs were used for prevention. In HSV-2, IFN-y inhibited
the virus reproduction, like in the treatment scheme, within the dilution range of 1 : 50 to 1 : 500, whereas
TNF-T was effective in the range of 1 : 50. In CMV, the drugs' effect, when used for prevention, was similar to that
observed in the treatment scheme. The highest inhibition values were registered for HSV-2, when it was used 24
hours before infection (IFN-y - 2.25 lg, dilution range of 1 : 50; TNF-2 -1.0 lg, dilution range of 1 : 50). IFN-y and
TNF-2 exert a synergic action on different stages of virus reproduction. A reliable additive effect was ensured in
prevention made 4 hour before infection by IFN-y and TNF-T only in experimental CMV infection.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).