Email this article Antiviral activity of human recombinant y-interferon and recombinant hybrid proteinof tumor necrosis factor-a-thymosin-сц on modelsof herpes-virus and cytomegalovirus infection invitro
- Issue: Vol 49, No 5 (2004)
- Pages: 37-40
- Section: Articles
- URL: https://virusjour.crie.ru/jour/article/view/11877
- ID: 11877
Cite item
Full Text
Abstract
The conducted studies showed a certain efficiency of gene-engineering preparation of IFN-y and TNF-T in virus
infections caused by herpes simplex virus 2 (HSV-2) and cytomegalovirus (CMV) in cell cultures of human embryo
fibroblast (HEF). The drugs have no viricidal action. IFN-y, when used according to the treatment scheme in vitro,
proved to be more effective versus TMF-T both in HSV-2 and in CMV. It inhibited significantly the HSV-2 reproduction
within the dilution range of 1: 50 to 1 : 500. It was also effective, when used in CMV, within the dilution range
of 1 : 5000 and lower, whereas TNF-T was effective within the range of 1 : 500 and lower as well as in 0.1 multiple
infection. A significantly higher effect was ensured when the drugs were used for prevention. In HSV-2, IFN-y inhibited
the virus reproduction, like in the treatment scheme, within the dilution range of 1 : 50 to 1 : 500, whereas
TNF-T was effective in the range of 1 : 50. In CMV, the drugs' effect, when used for prevention, was similar to that
observed in the treatment scheme. The highest inhibition values were registered for HSV-2, when it was used 24
hours before infection (IFN-y - 2.25 lg, dilution range of 1 : 50; TNF-2 -1.0 lg, dilution range of 1 : 50). IFN-y and
TNF-2 exert a synergic action on different stages of virus reproduction. A reliable additive effect was ensured in
prevention made 4 hour before infection by IFN-y and TNF-T only in experimental CMV infection.
infections caused by herpes simplex virus 2 (HSV-2) and cytomegalovirus (CMV) in cell cultures of human embryo
fibroblast (HEF). The drugs have no viricidal action. IFN-y, when used according to the treatment scheme in vitro,
proved to be more effective versus TMF-T both in HSV-2 and in CMV. It inhibited significantly the HSV-2 reproduction
within the dilution range of 1: 50 to 1 : 500. It was also effective, when used in CMV, within the dilution range
of 1 : 5000 and lower, whereas TNF-T was effective within the range of 1 : 500 and lower as well as in 0.1 multiple
infection. A significantly higher effect was ensured when the drugs were used for prevention. In HSV-2, IFN-y inhibited
the virus reproduction, like in the treatment scheme, within the dilution range of 1 : 50 to 1 : 500, whereas
TNF-T was effective in the range of 1 : 50. In CMV, the drugs' effect, when used for prevention, was similar to that
observed in the treatment scheme. The highest inhibition values were registered for HSV-2, when it was used 24
hours before infection (IFN-y - 2.25 lg, dilution range of 1 : 50; TNF-2 -1.0 lg, dilution range of 1 : 50). IFN-y and
TNF-2 exert a synergic action on different stages of virus reproduction. A reliable additive effect was ensured in
prevention made 4 hour before infection by IFN-y and TNF-T only in experimental CMV infection.
References
- Bach E. A., Aguet М., Schreiber R. D. The IFN gamma receptor: a paradigm for cytokine receptor signaling // Annu. Rev. Immunol. - 1997. - Vol. 15. - P. 563-591.
- Baskin H., Ellermann-Eriksen S., Lovmand J., Mogensen S. C. Herpes simplex virus type 2 synergizes with interferon-gamma in the induction of nitric oxide production in mouse macrophages through autocrine secretion of tumour necrosis factoralpha//J. Gen. Virol. - 1997. - Vol. 78, Pt 1. - P. 195-203.
- Chen S. H., Okes J. E., Lausch R. N. Synergistic antherpes effect of TNF-alpha and IFN-gamma in human corneal epithelial cells compared with that in corneal fibroblasts // Antiviral Res. - 1994. - Vol. 25, N 3-4. - P. 201-213.
- Costa-Pereira A. P., Williams Т. M., Strobl B. et al. The antiviral response to gamma interferon // J. Virol. - 2002. - Vol. 76, N 18. - P. 9060-9068.
- Feduchi E., Alonso M. A., Carrasco L. Human gamma interferon and tumor necrosis factor exert a synergistic blockade on the replication of herpes simplex virus // J. Virol. - 1989. - Vol. 63, N 3. - P. 1354-1359.
- Feduchi E., Carrasco L. Mechanism of inhibition of HSV-1 replication by tumor necrosis factr and interferon gamma // Virology. - 1991. - Vol. 180, N 2. - P. 822-825.
- Lee A. H., Hong J. H., Seo Y. S. Tumour necrosis factor-alpha and interferon-gamma synergistically activate the RANTES promoter through nuclear factor kappaB and interferon regulatory factor 1 (IRF-1) transcription factors // Biochem. J. - 2000. - Vol. 350, Pt 1. - P. 131-138.
- Mestan J., Digel W., Mittnacht S. et al. Antiviral effects of recombinant tumour necrosis factor in vitro // Nature. - 1986. - Vol. 323, N 6091. - P. 816-819.
- Presti R. M., Popkin D. L., Connick M. et al. Novel cell type-specific antiviral mechanism of interferon gamma action in macrophages // J. Exp. Med. - 2001. - Vol. 193, N 4. - P. 483-496.
- Samuel С. Е. Antiviral actions of interferons // Clin. Microbiol. Rev. - 2001. - Vol. 14, N 4. - P. 778-809.
- Sen G. C. Viruses and interferons // Annu. Rev. Microbiol. - 2001. - Vol. 55. - P. 255-281.
- Stark G. R., Kerr I. M., Williams B. R. et al. How cells respond to interferons // Annu. Rev. Biochem. - 1998. - Vol. 67. - P. 227-264.
- Taylor J. L., Little S. D., O'Brien W. J. The comparative antiherpes simplex virus effects of human interferons // J. Interferon Cytokine Res. - 1998. - Vol. 18, N 3. - P. 159-165.
- Wong G. H., Goeddel D. V. Tumour ncrosis factors alpha and beta inhibit virus replication and synergize with interferons // Nature. - 1986. - Vol. 323, N 6091. - P. 819-822.
- Zhang H., Fu S., Busch A., Chen F. et al. Identification of TNF- alpha-sensitive sites in HCMViel promoter // Exp. Mol. Pathol. - 2001. - Vol. 71, N 2. - P. 106-114.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).