MOLECULAR AND BIOLOGICAL CHARACTERISTICS OF VACCINARY ERA-CB 20M OF RABIES VIRUS

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Abstract

The molecular and biological characteristics of the vaccine against rabies virus strain ERA-CB 20M obtained by the Russian rabiologist, doctor of medical sciences S.V. Gribencha by adapting and cloning the strain ERA and SAD in a transplantable BHK-21 C13 cell culture are presented. The spectrum of the most sensitive strain of rabies ERA-CB 20M cell lines was determined and the level of glycoprotein was quantitatively determined. Primary nucleotide sequences of fragments of the genome of the strain ERA-CB 20M (genes N and G) were obtained and phylogenetic analysis was carried out. Molecular analysis showed that this strain belongs to the group of vaccine strains SAD1. When compared with the reference strain SAD1, 10% of the nucleotide differences were revealed in the gene fragment N; 15%, in the gene fragment G.

About the authors

M. A. Losich

D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Diagnostic and Prevention Research Institute for Human and Animal Diseases

Author for correspondence.
Email: mkohnovich@rambler.ru
Russian Federation

O. N. Zaykova

D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Diagnostic and Prevention Research Institute for Human and Animal Diseases

Email: noemail@neicon.ru
Russian Federation

I. V. Nepoklonova

Diagnostic and Prevention Research Institute for Human and Animal Diseases

Email: noemail@neicon.ru
Russian Federation

T. V. Grebennikova

D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Peoples’ Friendship University of Russia

Email: noemail@neicon.ru
Russian Federation

O. A. Verkhovsky

Diagnostic and Prevention Research Institute for Human and Animal Diseases

Email: noemail@neicon.ru
Russian Federation

A. I. Odnovorov

Peoples’ Friendship University of Russia

Email: noemail@neicon.ru
Russian Federation

T. I. Aliper

D.I. Ivanovsky Institute of Virology, «National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Diagnostic and Prevention Research Institute for Human and Animal Diseases

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2018 Losich M.A., Zaykova O.N., Nepoklonova I.V., Grebennikova T.V., Verkhovsky O.A., Odnovorov A.I., Aliper T.I.

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