Cytokine-regulating activity of anti-virus preparation CelAgripus in Burkitt's lym-phoma stable B-cell lines

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Abstract

Introduction. Cytokines activated in response to immunosuppressive viral infections can directly or indirectly affect the neoplastic transformation of B cells. In this study, we studied a new substance designed to produce the antiviral drug CelAgrip (CA, CelAgripus), which exhibits interferon (IFN) and cytokine-inducing activity and, apparently, can be used as an activator of antiviral immunity.

Purpose - is to evaluate the cytokine-regulating effect of CA in Burkitt's lymphoma (LB) cell lines latently infected with the Epstein-Barr virus (EBV).

Objectives: to study the CA-induced expression of the cytokine genes IL-ip, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IFN-a, IFN -Y, IFN-p, IFN-A1, IFN-A2, IFN-A3, TNF-a in normal and EBV transformed LB cells.

Material and methods. Cell line: the human embryo fibroblasts (HEF), Namalva, Daudi, Raji, P3HR-1. Preparations: CA, gossypol-acetic acid (GAA), sodium carboxymethyl cellulose (Na-CMC). Methods: RT-PCR and methods for assessing cytotoxicity (MTT and Scepter 2.0 Merck cell counter).

Results. The effect of the CA preparation on the expression of IFN-Л, IL-1p, IL-6, IL-8 and IL-10 genes was revealed. Discussion. We observed the activation of gene expression of IFN-A, IL-1P, IL-6, IL-8 and suppression of IL-10 gene activity when treatment CA of LB cells.

Conclusion. The substance CA has new effects on the activation of the expression of a number of key cytokine genes in stable Burkitt lymphoma cell lines.

About the authors

A. N. Narovlyansky

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Author for correspondence.
Email: narovl@yandex.ru
ORCID iD: 0000-0003-0601-7148

Alexander N. Narovlyansky - DBS, Chief Researcher of the Cytokine Laboratory.

Moscow, 123098.

Россия

M. V. Mezentseva

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0001-7346-5536

Moscow, 123098.

Россия

I. A. Suetina

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0003-2878-0590

Moscow, 123098.

Россия

L. I. Russu

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0001-6353-9917

Moscow, 123098.

Россия

A. M. Ivanova

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-6008-7967

Moscow, 123098.

Россия

V. V. Poloskov

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0003-0001-2493

Moscow, 123098.

Россия

A. V. Izmest'eva

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-0035-324X

Moscow, 123098.

Россия

T. P. Ospelnikova

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-1580-6096

Moscow, 123098.

Россия

A. A. Sarymsakov

Institute of Polymer Chemystry and Physics Uzbek Academy of Sciences

Email: fake@neicon.ru
ORCID iD: 0000-0003-4562-7280

Tashkent, 100128.

Узбекистан

F. I. Ershov

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F.Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-4780-7560

Moscow, 123098.

Россия

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Copyright (c) 2019 Narovlyansky A.N., Mezentseva M.V., Suetina I.A., Russu L.I., Ivanova A.M., Poloskov V.V., Izmest'eva A.V., Ospelnikova T.P., Sarymsakov A.A., Ershov F.I.

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