Assessment of immunogenic activity of the cloned human rotavirus A WA strain

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Abstract

Introduction. Rotovirus infection (RVI) caused by the dsRNA-containing virus from genus Rotavirus, Reoviridae family, belonging to group A (RVA), is the cause of severe diarrhea in human and other mammalian species. Vaccination is the most effective way to reduce the incidence of RVI. At present, the effectiveness of using gnotobiotic piglets as a universal model for reproducing human rotavirus infection and assessing the quality of RVI vaccine preparations has been experimentally proven.

Goals and objectives. Evaluation of immunogenic activity of the cloned RVA Wa strain in the newborn Vietnamese pot-bellied piglets trial.

Material and methods. Development of viral preparations of the cloned human Wa strain PBA, development of human RVA rVP6, ELISA, polymerase chain reaction with reverse transcription, immunization and experimental infection of newborn piglets.

Results. The article presents the results of the experiment on double immunization of newborn piglets with native virus preparations with the infection activity 5.5 lg TCID50/ml, 3 cm3 per dose, HRV with adjuvant 500 pig per dose and mock preparation (control group) followed with experimental inoculation of all animals with virulent virus strain Wa G1P[8] human RVA with infectious activity of 5.5 lg TCID50/ml in 5 cm3 dose. Development of clinical signs of disease and animal death were observed only in control group. RT-PCR system to detect RVA RNA in rectal swabs, samples of small intestine and peripheral lymph nodes was developed. ELISA based on obtained human RVA rVP6 was developed and results on RVA-specific IgG-antibodies in serum samples of experimental piglets are presented.

Conclusion. In the course of the research, a high immunogenic activity of the native and purified virus of the cloned Wa RVA strain Wa was established and the possibility of its use as the main component of the RVI vaccine was confirmed. The possibility of using conventional newborn pigs instead of gnotobiotic piglets as an experimental model was demonstrated.

About the authors

O. E. Latyshev

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Author for correspondence.
Email: oleglat80@mail.ru
ORCID iD: 0000-0002-5757-3809

Oleg E. Latyshev - PhD, Senior Scientist, Laboratory of molecular diagnostics.

 

Moscow, 123098.

Russian Federation

O. V. Eliseeva

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-0723-9749

Moscow, 123098.

Russian Federation

L. V. Kostina

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-9556-1454

Moscow, 123098.

Russian Federation

K. P. Alekseev

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0001-9536-3127

Moscow, 123098.

Russian Federation

K. M. Khametova

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-8461-600X

Moscow, 123098.

Russian Federation

E. G. Altaeva

Diagnostics and Prevention Research Institute for Human and Animal Diseases

Email: fake@neicon.ru
ORCID iD: 0000-0001-7188-1704

Moscow, 123098.

Russian Federation

O. A. Verkhovsky

Diagnostics and Prevention Research Institute for Human and Animal Diseases

Email: fake@neicon.ru
ORCID iD: 0000-0003-0784-9341

Moscow, 123098.

Russian Federation

T. I. Aliper

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-5876-2425

Moscow, 123098.

Russian Federation

T. V. Grebennikova

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya; Peoples Frendship University of Russia

Email: fake@neicon.ru

Moscow, 123098;Moscow, 117198.

Russian Federation

References

  1. Львов Д.К., ред. Руководство по вирусологии: Вирусы и вирусные инфекции человека и животных. М.: МИА; 2013.
  2. Алипер Т.И., ред. Актуальные инфекционные болезни свиней: Руководство для студентов, научных и практических специалистов. М.: ЗооВетКнига; 2019.
  3. Gentsch J.R., Laird A.R., Bielfelt B., Griffin D.D., Banyai K., Ramachandran M., et al. Serotype diversity and reassortment between human and animal rotavirus strains: implications for rotavirus vaccine programs. J. Infect. Dis. 2005; 192(Suppl. 1): 146-59. Doi: https://doi.org/10.1086/431499
  4. Matthijnssens J., Heylen E., Zeller M., Rahman M., Lemey P., Van Ranst M. Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol. Biol. Evol. 2010; 27(10): 2431-6. Doi: https://doi.org/10.1093/molbev/msq137
  5. Зайцева Е.В., Ольнева Т.А., Кулешов К.В., Подколзин А.Т., Шипулин Г.А., Кондратьева Л.М. и др. Результаты мониторинга антигенных типов ротавирусов гр. А на территории Российской Федерации в период 2011-2015 гг. Клиническая лабораторная диагностика. 2016; 61(7): 445-8. Doi: https://doi.org/10.18821/0869-2084-2016-61-7-445-448
  6. Алексеев К.П., Кальнов С.Л., Гребенникова Т.В., Алипер Т.И. Ротавирусная инфекция человека. Стратегии вакцинопрофилактики. Вопросы вирусологии. 2016; 61(3): 154-9.
  7. Денисюк Н.Б. Генетическая характеристика ротавирусов группы А, циркулирующих в Оренбургском регионе в сезон 2016¬2017 гг. Детские инфекции. 2017; 16(4): 42-5. Doi: https://doi.org/10.22627/2072-8107-2017-16-4-42-45 15.
  8. Zhang J., Liu H., Jia L., Payne D.C., Hall A.J., Xu Z., et al. Active, population-based surveillance for rotavirus gastroenteritis in Chinese children: Beijing Municipalityand Gansu Province, China. Pediatr. Infect. Dis. J. 2015; 34(1): 40-6. Doi: https://doi.org/10.1097/INF.0000000000000505
  9. Gomez M.M., da Silva M.F.M., Volotao E.M., Fialho A.M., Mazzoco C.S., Rocha M.S., et. al. G26P[19] rotavirus A strain causing acute gastroenteritis in the American continent. Mem. Inst. Oswaldo Cruz. 2018; 113(12): e180344. Doi: http://dx.doi.org/10.1590/0074-02760180344
  10. My P.V., Rabaa M.A., Donato C., Cowley D., Phat V.V., Dung T.T., et. al. Novel porcine-like human G26P[19] rotavirus identified in hospitalized paediatric diarrhoea patients in Ho Chi Minh City, Vietnam. J. Gen. Virol. 2014; 95(12): 2727-33. Doi: https://doi.org/10.1099/vir.0.068403-0
  11. PATH (2011-2016). Available at: https://www.path.org/programs/center-for-vaccine-innovation-and-access/rotaflash/
  12. Burnett E., Jonesteller C.L., Tate J.E., Yen C., Parashar U.D. Global impact of rotavirus vaccination on childhood hospitalizations and mortality from diarrhea. J. Infect. Dis. 2017; 215(11): 1666-72. Doi: https://doi.org/10.1093/infdis/jix186
  13. Crawford S.E., Ramani S., Tate J.E., Parashar U.D., Svensson L., Hagbom M., et al. Rotavirus infection. Nat. Rev. Dis. Primers. 2017; 3: 17083. Doi: https://doi.org/10.1038/nrdp.2017.83
  14. Tate J.E., Parashar U.D. Rotavirus vaccines in routine use. Clin. Infect. Dis. 2014; 59(9): 1291-301. Doi: https://doi.org/10.1093/cid/ciu564
  15. Хаметова К.М., Алексеев К.П., Южаков А.Г., Костина Л.В., Раев С.А., Мусиенко М.И. и др. Молекулярно-биологические свойства клонированного штамма Wa ротавируса А человека. Вопросы вирусологии. 2019; 64(1): 16-22.
  16. Ward L.A., Rosen B.I., Yuan L., Saif L.J. Pathogenesis of an attenuated and a virulent strain of group A human rotavirus in neonatal gnotobiotic pigs. J. Gen. Virol. 1996; 77(Pt. 7): 1431-41. Doi: https://doi.org/10.1099/0022-1317-77-7-1431
  17. Friess A.E., Sinowatz F., Skolek-Winnisch R., Trautner W. The placenta of the pig. II. The ultrastructure of the areolae. Anat. Embryol. (Berl.). 1981; 163(1): 43-53. Doi: https://doi.org/10.1007/bf00315769
  18. Lala P.K., Chatterjee-Hasrouni S., Kearns M., Montgomery B., Colavincenzo V. Immunobiology of the feto-maternal interface. Immunol. Rev. 1983; 75: 87-116. Doi: https://doi.org/104111/j.1600-065X.1983.tb01092.x
  19. Butler J.E., Lemke C.D., Weber P., Sinkora M., Lager K.M. Antibody repertoiredevelopment in fetal and neonatal piglets: XIX. Undiversified B cells with hydrophobic HCDR3s preferentially proliferate in the porcine reproductive and respiratory syndrome. J. Immunol. 2007; 178(10): 6320-31. Doi: https://doi.org/10.4049/jimmunol.178.10.6320
  20. Saif L.J., Fernandez F.M. Group A rotavirus veterinary vaccines. J. Infect. Dis. 1996; 174(Suppl. 1): 98-106. Doi: https://doi.org/10.1093/infdis/174.Supplement_1.S98
  21. Yuan L., Saif L.J. Induction of mucosal immune responses and protection against enteric viruses: rotavirus infection of gnotobiotic pigs as a model. Vet. Immunol. Immunopathol. 2002; 87(3-4): 147-60. Doi: https://doi.org/10.1016/S0165-2427(02)00046-6
  22. Hammerberg C., Schurig G.G., Ochs D.L. Immunodeficiency in young pigs. Am. J. Vet. Res. 1989; 50(6): 868-74.
  23. Azevedo M.P., Vlasova A.N., Saif L.J. Human rotavirus virus-like particle vaccines evaluated in a neonatal gnotobiotic pig model of human rotavirus disease. Expert. Rev. Vaccines. 2013; 12(2): 169-81. Doi: https://doi.org/10.1586/erv.13.3
  24. Babji S., Arumugam R., Sarvanabhavan A., Gentsch J.R., Kang G. Approach to molecular characterization of partially and completely untyped samples in an Indian rotavirus surveillance program. Vaccine. 2014; 32(Suppl. l): A84-8. Doi: https://doi.org/10.1016/j.vaccine.2014.04.024
  25. Gentsch J.R., Glass R.I., Woods P., Gouvea V, Gorziglia M., Flores J., et al. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J. Clin. Microbiol. 1992; 30(6): 1365-73.
  26. Close B., Banister K., Baumans V, Bernoth E.M., Bromage N., Bunyan J., et al. Recommendations for euthanasia of experimental animals: Part 2. DGXT of the European Commission. Lab. Anim. 1997; 31(1): 1-32. Doi: https://doi.org/10.1258/002367797780600297
  27. Сергеев В.А., Непоклонов Е.А., Алипер Т.И. Вирусы и вирусные вакцины. М.: Библионика; 2007.
  28. Yuan L., Ward L.A., Rosen B.I., To T.L., Saif L.J. Systematic and intestinal antibodysecreting cell responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of dis-ease. J. Virol. 1996; 70(5): 3075-83.
  29. Burns J.W., Krishnaney A.A., Vo P.T., Rouse R.V., Anderson L.J., Greenberg H.B. Analyses of homologous rotavirus infection in the mouse model. Virology. 1995; 207(1): 143-53. Doi: https://doi.org/10.1006/viro.1995.1060
  30. Patel M., Shane A.L., Parashar U.D., Jiang B., Gentsch J.R., Glass R.I. Oral rotavirus vaccines: how well will they work where they are needed most? J. Infect. Dis. 2009; 200(Suppl. 1): 39-48. Doi: https://doi.org/10.1086/605035
  31. Wagstrom E.A., Yoon K.J., Zimmerman J.J. Immune components in porcine mammary secretions. Viral Immunol. 2000; 13(3): 383-97. Doi: https://doi.org/10.1089/08828240050144699

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Copyright (c) 2019 Latyshev O.E., Eliseeva O.V., Kostina L.V., Alekseev K.P., Khametova K.M., Altaeva E.G., Verkhovsky O.A., Aliper T.I., Grebennikova T.V.

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