Assessment of immunogenic activity of the cloned human rotavirus A WA strain

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Abstract

Introduction. Rotovirus infection (RVI) caused by the dsRNA-containing virus from genus Rotavirus, Reoviridae family, belonging to group A (RVA), is the cause of severe diarrhea in human and other mammalian species. Vaccination is the most effective way to reduce the incidence of RVI. At present, the effectiveness of using gnotobiotic piglets as a universal model for reproducing human rotavirus infection and assessing the quality of RVI vaccine preparations has been experimentally proven.

Goals and objectives. Evaluation of immunogenic activity of the cloned RVA Wa strain in the newborn Vietnamese pot-bellied piglets trial.

Material and methods. Development of viral preparations of the cloned human Wa strain PBA, development of human RVA rVP6, ELISA, polymerase chain reaction with reverse transcription, immunization and experimental infection of newborn piglets.

Results. The article presents the results of the experiment on double immunization of newborn piglets with native virus preparations with the infection activity 5.5 lg TCID50/ml, 3 cm3 per dose, HRV with adjuvant 500 pig per dose and mock preparation (control group) followed with experimental inoculation of all animals with virulent virus strain Wa G1P[8] human RVA with infectious activity of 5.5 lg TCID50/ml in 5 cm3 dose. Development of clinical signs of disease and animal death were observed only in control group. RT-PCR system to detect RVA RNA in rectal swabs, samples of small intestine and peripheral lymph nodes was developed. ELISA based on obtained human RVA rVP6 was developed and results on RVA-specific IgG-antibodies in serum samples of experimental piglets are presented.

Conclusion. In the course of the research, a high immunogenic activity of the native and purified virus of the cloned Wa RVA strain Wa was established and the possibility of its use as the main component of the RVI vaccine was confirmed. The possibility of using conventional newborn pigs instead of gnotobiotic piglets as an experimental model was demonstrated.

About the authors

O. E. Latyshev

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Author for correspondence.
Email: oleglat80@mail.ru
ORCID iD: 0000-0002-5757-3809

Oleg E. Latyshev - PhD, Senior Scientist, Laboratory of molecular diagnostics.

 

Moscow, 123098.

Russian Federation

O. V. Eliseeva

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-0723-9749

Moscow, 123098.

Russian Federation

L. V. Kostina

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-9556-1454

Moscow, 123098.

Russian Federation

K. P. Alekseev

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0001-9536-3127

Moscow, 123098.

Russian Federation

K. M. Khametova

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-8461-600X

Moscow, 123098.

Russian Federation

E. G. Altaeva

Diagnostics and Prevention Research Institute for Human and Animal Diseases

Email: fake@neicon.ru
ORCID iD: 0000-0001-7188-1704

Moscow, 123098.

Russian Federation

O. A. Verkhovsky

Diagnostics and Prevention Research Institute for Human and Animal Diseases

Email: fake@neicon.ru
ORCID iD: 0000-0003-0784-9341

Moscow, 123098.

Russian Federation

T. I. Aliper

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-5876-2425

Moscow, 123098.

Russian Federation

T. V. Grebennikova

National Research Center for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya; Peoples Frendship University of Russia

Email: fake@neicon.ru

Moscow, 123098;Moscow, 117198.

Russian Federation

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Copyright (c) 2019 Latyshev O.E., Eliseeva O.V., Kostina L.V., Alekseev K.P., Khametova K.M., Altaeva E.G., Verkhovsky O.A., Aliper T.I., Grebennikova T.V.

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