RETRACTED: High-throughput sequencing in diagnostics and prevention of herpes simplex virus infection (Herpesviridae, Alphaherpesvirinae, Simplexvirus, Human alphaherpesvirus 1)

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Abstract

RETRACTED

Herpes simplex viruses types 1 (HSV-1) and 2 (HSV-2) are among the most common viruses in the human population. The clinical manifestations of HSV infection vary widely, which necessitates reliable molecular methods for the timely diagnosis of herpes virus infection, as well as for detection of mutations in the genes responsible for drug resistance. PCR is often unable to detect HSV isolates with nucleotide substitutions at the primer binding site. Sanger sequencing of the whole genome reveals mutations mainly at the consensus level, which accumulate at advanced stages of viral infection. High-throughput sequencing (HTS, next generation sequencing) offers an obvious advantage both in early diagnosis of herpes virus infection and identification of HSV variants.

About the authors

O. V. Sergeyev

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: osergeyev123@gmail.com
ORCID iD: 0000-0003-3407-2224

Oleg V. Sergeyev, Doct. Sci. (Biol.), Professor at the Department of microbiology, virology and immunology

Moscow, 119991

Russian Federation

R. E. Bosh’ian

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Email: fake@neicon.ru
ORCID iD: 0000-0003-4789-4964

Roman E. Bosh’ian

Moscow, 119991

Russian Federation

I. F. Barinsky

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Email: fake@neicon.ru
ORCID iD: 0000-0002-9909-3598

Igor F. Barinsky

Moscow, 123098

Russian Federation

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