Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease

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  • Authors: Vitkova O.N.1, Kapustina O.V.2, Lobova T.P.1, Mikhailova V.V.1, Safonov G.A.3, Vlasova N.N.4, Belousova R.V.5
  • Affiliations:
    1. Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance
    2. All-Russia Scientific-Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences
    3. Russian Academy of Sciences
    4. Federal Center for Animal Health Protection, Federal Service for Veterinary and Phytosanitary Surveillance
    5. Moscow State Academy of Veterinary Medicine and Biotechnology, Ministry of Agriculture of the Russian Federation
  • Issue: Vol 60, No 6 (2015)
  • Pages: 41-45
  • Section: ORIGINAL RESEARCH
  • Submitted: 12.07.2020
  • Accepted: 12.07.2020
  • Published: 28.12.2015
  • URL: https://virusjour.crie.ru/jour/article/view/379
  • ID: 379

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Abstract

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno-chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.

About the authors

O. N. Vitkova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Author for correspondence.
Email: olgavitkova@mail.ru

Ol’ga Vitkova , Candidate of veterinary Sciences

111662, Moscow

Russian Federation

O. V. Kapustina

All-Russia Scientific-Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences

Email: fake@neicon.ru
601120, Pokrov Russian Federation

T. P. Lobova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
111662, Moscow Russian Federation

V. V. Mikhailova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
111662, Moscow Russian Federation

G. A. Safonov

Russian Academy of Sciences

Email: fake@neicon.ru
117334, Moscow Russian Federation

N. N. Vlasova

Federal Center for Animal Health Protection, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
600901, Vladimir Russian Federation

R. V. Belousova

Moscow State Academy of Veterinary Medicine and Biotechnology, Ministry of Agriculture of the Russian Federation

Email: fake@neicon.ru
109472, Moscow Russian Federation

References

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  7. Peiris J.S., Tu W.W., Yen H.L. A novel H1N1 virus causes the first pandemic of the 21st century. Eur. J. Immunol. 2009; 39: 2946–54.
  8. O.I.E. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th ed. Paris; 2008.
  9. Владимиров Ю.А. Активированная хемилюминисценция и биолюминисценция как инструмент в медико-биологических исследованиях. Соросовский образовательный журнал. 2001; 7 (1): 16–23.
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Copyright (c) 2015 Vitkova O.N., Kapustina O.V., Lobova T.P., Mikhailova V.V., Safonov G.A., Vlasova N.N., Belousova R.V.

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