Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease

Cover Page
  • Authors: Vitkova O.N.1, Kapustina O.V.2, Lobova T.P.1, Mikhailova V.V.1, Safonov G.A.3, Vlasova N.N.4, Belousova R.V.5
  • Affiliations:
    1. Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance
    2. All-Russia Scientific-Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences
    3. Russian Academy of Sciences
    4. Federal Center for Animal Health Protection, Federal Service for Veterinary and Phytosanitary Surveillance
    5. Moscow State Academy of Veterinary Medicine and Biotechnology, Ministry of Agriculture of the Russian Federation
  • Issue: Vol 60, No 6 (2015)
  • Pages: 41-45
  • Section: ORIGINAL RESEARCH
  • Submitted: 12.07.2020
  • Accepted: 12.07.2020
  • Published: 28.12.2015
  • URL: https://virusjour.crie.ru/jour/article/view/379
  • ID: 379

Cite item

Full Text

Abstract

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno-chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.

About the authors

O. N. Vitkova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Author for correspondence.
Email: olgavitkova@mail.ru

Ol’ga Vitkova , Candidate of veterinary Sciences

111662, Moscow

Россия

O. V. Kapustina

All-Russia Scientific-Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences

Email: fake@neicon.ru
601120, Pokrov Россия

T. P. Lobova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
111662, Moscow Россия

V. V. Mikhailova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
111662, Moscow Россия

G. A. Safonov

Russian Academy of Sciences

Email: fake@neicon.ru
117334, Moscow Россия

N. N. Vlasova

Federal Center for Animal Health Protection, Federal Service for Veterinary and Phytosanitary Surveillance

Email: fake@neicon.ru
600901, Vladimir Россия

R. V. Belousova

Moscow State Academy of Veterinary Medicine and Biotechnology, Ministry of Agriculture of the Russian Federation

Email: fake@neicon.ru
109472, Moscow Россия

References

  1. Львов Д.К. Новые и возвращающиеся инфекции – дремлющий вулкан. Проблемы особо опасных инфекций. 2008; 2 (96): 5–8.
  2. Силко Н.Ю., Глущенко Ю.Г., Шестопалова Л.В., Юрченко К.С. Биологические свойства велегенных штаммов вируса болезни Ньюкасла, изолированных от птиц на Северном Кавказе. Вопросы вирусологии. 2013; 58 (1): 45–8.
  3. Umali D.V., Ito H., Suzuki T., Shirota K., Katoh H., Ito T. Molecular epidemiology of Newcastle disease virus isolates from vaccinated commercial poultry farms in non-epidemic areas of Japan. Virol. J. 2013, 10:330.
  4. Aldous E.W., Mynn J.K., Banks J., Alexander D.J. A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathol. 2003; 32 (3): 239–56.
  5. Fiore A.E., Shay D.K., Broder K., Iskander J.K., Uyeki T.M., Mootrey G. et al. Prevention and control of influenza: Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2008. MMWR Recomm. Rep. 2008; 57 (RR-7): 1–60.
  6. Naffakh N., Tomoiu A., Rameix-Welti M.A., van der Werf S. Host restriction of avian influenza viruses at the level of the ribonucleoproteins. Annu. Rev. Microbiol. 2008; 62: 403–24.
  7. Peiris J.S., Tu W.W., Yen H.L. A novel H1N1 virus causes the first pandemic of the 21st century. Eur. J. Immunol. 2009; 39: 2946–54.
  8. O.I.E. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th ed. Paris; 2008.
  9. Владимиров Ю.А. Активированная хемилюминисценция и биолюминисценция как инструмент в медико-биологических исследованиях. Соросовский образовательный журнал. 2001; 7 (1): 16–23.
  10. Barnard G.J.R., Kim J.B., Williams J.L. Alternative Immunoassays. New York; 1985: 123–52.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Vitkova O.N., Kapustina O.V., Lobova T.P., Mikhailova V.V., Safonov G.A., Vlasova N.N., Belousova R.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies