Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease

O. N. Vitkova; Виткова О. Н.; O. V. Kapustina; Капустина О. В.; T. P. Lobova; Лобова Т. П.; V. V. Mikhailova; Михайлова В. В.; G. A. Safonov; Сафонов Г. А.; N. N. Vlasova; Власова Н. Н.; R. V. Belousova; Белоусова Р. В.

Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease

Authors: Vitkova O.N.¹, Kapustina O.V.², Lobova T.P.¹, Mikhailova V.V.¹, Safonov G.A.³, Vlasova N.N.⁴, Belousova R.V.⁵
Affiliations:
1. Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance
2. All-Russia Scientific-Research Institute of Veterinary Virology and Microbiology, Russian Academy of Agricultural Sciences
3. Russian Academy of Sciences
4. Federal Center for Animal Health Protection, Federal Service for Veterinary and Phytosanitary Surveillance
5. Moscow State Academy of Veterinary Medicine and Biotechnology, Ministry of Agriculture of the Russian Federation
Issue: Vol 60, No 6 (2015)
Pages: 41-45
Section: ORIGINAL RESEARCH
URL: https://virusjour.crie.ru/jour/article/view/379
ID: 379

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Abstract

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno-chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.

Keywords

enzyme immunoassay, chemiluminescence detection, immuno-chemiluminescence analysis, enzyme-linked immunosorbent assay, avian influenza, Newcastle disease, horseradish peroxidase, luminometric method, luminol

About the authors

O. N. Vitkova

Central Scientific-Methodological Veterinary Laboratory, Federal Service for Veterinary and Phytosanitary Surveillance

Author for correspondence.
Email: olgavitkova@mail.ru

Ol’ga Vitkova , Candidate of veterinary Sciences

111662, Moscow

Russian Federation

References

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