Experimental model of secondary bacterial pneumonia after influenza

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Abstract

In this work, we developed and characterized a mouse model of the secondary bacterial pneumonia after influenza. Specifically, the S. aureus 1986 given intranasally to the mice infected intranasally with the A/California/04/2009 (H1N1 pndm) virus resulted in greater mortality, weight loss, and reduction of mean life time as compared with infection with either pathogen alone. These data were confirmed by the virological and microbiological studies. Both viral and bacterial lung titers were enhanced during coinfections compared with single infections. In addition, preceding influenza infection resulted in a decreased clearance of the virus and S. aureus in lungs of mice. These findings indicate that the influenza virus exacerbates the S. aureus pneumonia and its pathogen factors in mice.

About the authors

I. А. Leneva

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
Moscow Россия

E. I. Leonova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
Moscow Россия

N. R. Маkhmudоvа

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
Moscow Россия

I. N. Falynskova

I.I. Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Email: falynskova@mail.ru

Irina Falynskova, research fellow

Moscow

Россия

I. Т. Fedyakina

D.I. Ivanovsky Institute of Virology «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»

Email: fake@neicon.ru
Moscow Россия

V. V. Zverev

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
Moscow Россия

N. A. Mikhailova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
Moscow Россия

References

  1. Грипп. Информационный бюллетень ВОЗ. 2014; 211. Available at: http://www.who.int/mediacentre/factsheets/fs211/ru/
  2. Legand A., Briand S., Shindo N., BrooksW.A., de Jong M.D., Farrar J. et al. Addressing the public health burden of respiratory viruses: the Battle against Respiratory Viruses (BRaVe) Initiative. Future Virology. 2013, 8 (10): 953–68.
  3. L’vov D.K., Shchelkanov M.Y., Prilipov A.G., Vlasov N.A., Fedyakina I.T., Deryabin P.G. et al. Evolution of highly pathogenic avian influenza H5N1 virus in natural ecosystems of northern Eurasia (2005-08). Avian Dis. 2010. 54 (1): 483–95.
  4. Бухарин О. В., Паньков А. С., Усвяцов Б. Я., Скворцов В. О. Модификация свойств вируса гриппа и Staphylococcus aureus в условиях их ассоциации. Эпидемиология и вакцинопрофилактика. 2011; 3 (58): 24–8.
  5. Potter C.W. Chronicle of influenza pandemics. In: Nicholson K.G., Webster R.G., Hay A.J., eds. Textbook of influenza. London: Blackwell Scientific Publications; 1998: 3-18.
  6. Morens D.M., Taubenberger J.K., Fauci A.S. Predominant role of bacterial pneumonia as a cause of death in pandemic influenza: implications for pandemic influenza preparedness. J. Infect. Dis. 2008; 198 (7): 962-70.
  7. Murray R.J., Robinson J.O., White J.N., Hughes F., Coombs G.W., Pearson J.C. et al. Community-acquired pneumonia due to pandemic A (H1N1)2009 influenzavirus and methicillin resistant Staphylococcus aureus co-infection. PLoS One. 2010; 5 (1): e8705.
  8. Centers for Disease Control and Prevention. Bacterial coinfections in lung tissue specimens from fatal cases of 2009 pandemic influenza A (H1N1)—United States, May-August 2009. MMWR Morb. Mortal. Wkly Rep. 2009; 58 (38): 1071–4.
  9. McCullers J.A., Rehg J.E. Lethal synergism between influenza virus and Streptococcus pneumoniae: characterization of a mouse model and the role of platelet-activating factor receptor. J. Infect. Dis 2002; 186 (3): 341–50.
  10. McCullers J.A. Insights into the interaction between influenza virus and pneumococcus. Clin. Microbiol. Rev. 2006; 19 (3): 571–82.
  11. McCullers J.A. Effect of antiviral treatment on the outcome of secondary bacterial pneumonia after influenza. J. Infect. Dis. 2004; 190 (3): 519–26.
  12. Iverson A.R, Boyd K.L, McAuley J.L., Plano L.R, Hart M.E, McCullers1 J A. Influenza virus primes mice for pneumonia from Staphylococcus aureus. J. Infect. Dis 2011; 203 (6): 880–8.
  13. Lee M.H., Arrecubieta C., Martin F.J., Prince A., Borczuk A.C., Lowy F.D. A postinfluenza model of Staphylococcus aureus pneumonia. J. Infect. Dis. 2010; 201 (4): 508–15.

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Copyright (c) 2015 Leneva I.А., Leonova E.I., Маkhmudоvа N.R., Falynskova I.N., Fedyakina I.Т., Zverev V.V., Mikhailova N.A.

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