The study of the antiviral activity of polyelectrolytes with respect to the influenza virus

Cover Page

Cite item

Full Text

Abstract

It is well known that polyelectrolytes (PE) have a damaging effect on the secondary structure of the proteins and enzymes. It is also known that some PEs exert an immunostimulating action on T- and B-lymphocytes. However, currently, almost nothing is known about the impact of the PE of the viral proteins and viral envelope. Therefore, a detailed study of the mechanisms of the antiviral action of various polyelectrolytes would create the scientific-practical base for the construction of the antiviral drugs on the polyelectrolyte basis. In these works, for the first time the influence of PE polystyrene sulfonate (PSS) with varying degrees of polymerization and polyallylamin (PAA) and with molecular mass 6 and 8 kDa on the infectivity of different strains of the influenza virus was studied. It was shown that the expressed antiviral action PSS with degree of polymerization 8 (ETS-8) and PAA (6 kDa) against the influenza viruses was characterized by a significant reduction in the infectious titer of the virus. It was determined that the span of the nontoxic concentrations for the ETS-8 was 1-40 mM; for PAA (6 kDa), 1-40 μM, with IC50 = 3.8 ± 0.19 mm and 1.8 ± 0.09 μM, respectively. For the first time the impact of the PE on the structural-functional state of the viral membrane was assessed on the basis of monomolecular monolayers used as models of cell membranes.

About the authors

N. A. Kontarov

I.I. Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Email: kontarov@mail.ru

Nikolay Kontarov, MD, PhD

105064, Moscow

Russian Federation

A. A. Ermakova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
105064, Moscow Russian Federation

N. S. Grebionkina

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
105064, Moscow Russian Federation

N. V. Yuminova

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
105064, Moscow Russian Federation

V. V. Zverev

I.I. Mechnikov Research Institute of Vaccines and Sera

Email: fake@neicon.ru
105064, Moscow Russian Federation

References

  1. Артюшенко С.В. Изучение механизмов ингибирующего действия полиэлектролитов в отношении парамиксо- и ортомиксовирусов (корь и грипп): Дисс. ... канд. биол. наук. М.; 2011.
  2. Haldar J., Deqiang A., Luis A., Chen J., Kabanov A.M. Polymeric coatings that inactivate both influenza virus and pathogenic bacteria. Cambridge: Departments of Chemistry, Biology, and Biological Engineering, and Center for Cancer Research, Massachusetts Institute of Technology; 2006.
  3. Slita A.V., Kasyanenko N.A., Nazarova О.V., Gavrilova I., Eropkina E.M., Sirotkin A.K. et al. DNA-polycation complexes: effect of polycation structure on physico-chemical and biological properties. J. Biothechnol. 2007; 127(4): 679–93.
  4. Stoll S., Chodanovski P. Polyelectrolite adsorption on an oppositely charged spherical particle. Chain rigidity effects. Macromolecules. 2002; 35: 9556–62.
  5. Хаитов Р.М., Некрасов А.В., Лыткина И.Н.. Иванова А.С., Пинегин Б.В. О влияние вакцинопрофилактики на уровни заболеваемости гриппа и ОРЗ. Журнал микробиологии. 1996; 3: 40–3.
  6. Изумрудов В.А., Зезин А.В., Кабанова В.А. Кинетика макромолекулярного обмена в растворах комплексов белков с полиэлектролитами. Доклады Aкадемии наук. 1986; 291(5): 1150–3.
  7. Изумрудов В.А., Нуркова Т.Ю., Зезин А.В., Кабанова В.А. Влияние длины цепи полианионов на направление и кинетику межполиэлектролитных реакций обмена. Высокомолекулярные соединения. 1987; 29: 474–8.
  8. Thompson W.R. Use of moving averages and interpolation to estimate median-effective dose; fundamental formulas, estimation of error, and relation to other methods. Bacteriol. Rev. 1947; 11(2): 115–45.
  9. Niks M., Otto M. Towards an optimized MTT assay. J. Immunol. Methods. 1990; 130(1): 149–51.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Kontarov N.A., Ermakova A.A., Grebionkina N.S., Yuminova N.V., Zverev V.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies