Email this article A study of the HIV-1 regulatory genes using the polymerase chain reaction
- Authors: Ryzhov K.A.1, Nosik M.N.1, Kravtchenko A.V.2
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Affiliations:
- Federal State Budgetary Institution «I.I. Mechnikov Research Institute for Vaccines and Sera»
- Federal Budget Institution of Science «Central Research Institute of Epidemiology» of the Federal Service on Customers' Rights Protection and Human Well-being Surveillance
- Issue: Vol 60, No 3 (2015)
- Pages: 41-44
- Section: ORIGINAL RESEARCH
- Submitted: 10.07.2020
- Accepted: 10.07.2020
- Published: 28.06.2015
- URL: https://virusjour.crie.ru/jour/article/view/333
- ID: 333
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Abstract
In this work, a total of 200 samples from the HIV-infected individuals were analyzed: 50 samples from the Saha Republic (Yakutia), 50 samples from the Vologda Region (City of Cherepovets), and 100 samples from the Moscow Region (Moscow and Moscow Region). All samples were obtained from the patients who were not undergoing antiretroviral therapy. It was detected that the regulatory genes vif, vpr, vpu, rev, tat, and nef were amplified with moderate sensitivity after one-stage amplification. When those samples were analyzed by the nested PCR the detection ratio was much higher. While studying nef-gene the phenomena of the splicing in cells cores was detected at the advanced stages of the HIV-infection (3 and 4 stages). At the same time, the splicing was not detected at the earlier stages of the HIV-infection. This effect might be the cause of the transition from asymptomatic stage of the infection to the advanced stage. It was also shown for the first time that the variability of the regulatory genes correlated with the virus subtype.
Keywords
About the authors
K. A. Ryzhov
Federal State Budgetary Institution «I.I. Mechnikov Research Institute for Vaccines and Sera»
Author for correspondence.
Email: rkazaw@yahoo.com
Konstantin Ryzhov, senior researcher
105064, Moscow
РоссияM. N. Nosik
Federal State Budgetary Institution «I.I. Mechnikov Research Institute for Vaccines and Sera»
Email: fake@neicon.ru
105064, Moscow Россия
A. V. Kravtchenko
Federal Budget Institution of Science «Central Research Institute of Epidemiology» of the Federal Service on Customers' Rights Protection and Human Well-being Surveillance
Email: fake@neicon.ru
111123, Moscow Россия
References
- Öhagen A., Gabuzda D. Role of Vif in Stability of the Human Immunodeficiency Virus Type 1 Core. J. Virol. 2000; 74(23): 11055–66.
- Raymond A.D., Campbell-Sims T.C., Khan M., Lang M., Huang M.B., Bond V.C. et al. HIV type 1 Nef is released from infected cells in CD45+ microvesicles and is present in the plasma of HIV-infected individuals. AIDS Res. Hum. Retrovir. 2011; 27(2): 167–78.
- Mologni D., Citterio P., Menzaghi B., Zanone Poma B., Riva C., Broggini V. et al. Vpr and HIV-1 disease progression: R77Q mutation is associated with long-term control of HIV-1 infection in different groups of patients. AIDS. 2006; 20(4): 567–74.
- Huigen M.C., Kamp W., Nottet H.S. Multiple effects of HIV-1 transactivator protein on the pathogenesis of HIV-1 infection. Eur. J. Clin. Investig. 2004; 34: 57–66.
- Boya P., Pauleau A.L., Poncet D., Gonzalez-Polo R.A., Zamzami N., Kroemer G. Viral proteins targeting mitochondria: Controlling cell death. Biochim. Biophys. Acta. 2004; 1659 (2–3): 178–89.
- Chase A., Zhou Y., Siliciano R.F. HIV-1-induced depletion of CD4+ T cells in the gut: Mechanism and therapeutic implications. Trends Pharmacol. Sci. 2006; 27: 4–7.
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