A panel of the drug-resistance HIV-1 clinical isolates

Cover Page

Cite item

Full Text

Abstract

A panel of 16 HIV-1 isolates was designed. Those isolates were isolated from patients undergoing HAART and developing resistance to the antiretroviral drugs. It was shown that the isolates were resistant to nucleoside RT inhibitors (retrovir, epivir) and non-nucleoside inhibitors (viramun). Isolates had stable replication activity. Average rate of cells expressing viral Ag was 14-20%. The infectious titer was 2.4 lg TCID50. The sequencing showed that all isolates were of the subtype A dominating in the major part of Russian Federation. This panel could be used as the biotechnological base for studying antiretroviral drugs of new generation and for the design of experimental vaccines.

About the authors

M. N. Nossik

I.I. Mechnikov Research institute for Vaccines and Sera

Author for correspondence.
Email: mnossik@yandex.ru

Marina Nosik, PhD

115088, Moscow

Россия

I. A. Kiseleva

I.I. Mechnikov Research institute for Vaccines and Sera

Email: fake@neicon.ru
115088, Moscow Россия

M. S. Bochkova

D.I. Ivanovsky Research Institute of Virology, Ministry of Health of the Russian Federation

Email: fake@neicon.ru
123098, Moscow Россия

K. A. Ryzhov

I.I. Mechnikov Research institute for Vaccines and Sera

Email: fake@neicon.ru
115088, Moscow Россия

A. V. Kravtchenko

Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: fake@neicon.ru
111123, Moscow Россия

V. V. Pokrovsky

Central Research Institute of Epidemiology, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Email: fake@neicon.ru
111123, Moscow Россия

References

  1. Alteri C., Svicher V., Gori C. et al. Characterization of the patterns of drug-resistance mutations in newly diagnosed HIV-1 infected patients naïve to the antiretroviral drugs. BMC Infect. Dis. 2009; 9: 111. doi: 10.1186/1471-2334-9-111.
  2. Hrazic M., Pellegrin I., Deveaue C. et al. Genotypic drug resistance during HIV-1-primary infection in France (1996–1999): frequency and response to treatment. AIDS. 2002; 16: 793–6.
  3. Lapadula G., Izzo I., Gargiulo F. et al. Updated prevalence of genotypic resistance among HIV-1 positive patients naïve to antiretroviral therapy: a single center analysis. J. Med. Virol. 2008; 80: 747–53.
  4. Little S., Holte S., Routy J. et al. Antiretroviral-drug resistance among patients recently infected with HIV. N. Engl. J. Med. 2002; 347: 385–94.
  5. Hirsch M.S., Gunthard H.F., Schapiro J.M. et al. Antiretroviral drug resistance testing in adult HIV-1 infection: 2008 recommendations of an international AIDS society-USA panel. Clin. Infect. Dis. 2008; 47: 266–85.
  6. Shafer R.W., Rhee S.Y., Bennett D.E. Consensus resistance mutations for epidemiological surveillance: basic principles and potential controversies. Antivir. Ther. 2008; 13: 59–68.
  7. WHO HIV drug resistance fact sheet. Available at: http://www.who.int/hiv/facts/drug_resistance/en/index.html
  8. Nosik M., Ryzhov K., Kravtchenko A. et al. Genotypic Analyses of HIV in antiretroviral drug-naïve patients from Moscow and Moscow Region, Russia. In: Proceedings 6th IAS conference on HIV pathogenesis, treatment and prevention. 2011, July 17–20; Rome; Italy: CDA002.
  9. Казеннова Е.В., Антонова О.В., Кузин С.Н. и др. Молекулярноэпидемиологическое исследование распространения ВИЧ-1 на территории Республики Саха (Якутия). Вопросы вирусологии. 2011; 5: 30–4.
  10. Рахманова А.Г., Петрова Л.В., Яковлев А.А. и др. Результаты определения резистентности ВИЧ к антиретровирусным препаратам у ВИЧ-инфицированных больных по материалам ГИБ № 30 им. С.П. Боткина. В кн.: 2-я конференция по вопросам ВИЧ/СПИД в Восточной Европе и Центральной Азии (ЕЕСААС 2008). 2008, Май 3–5; Москва. М.; 2008: 90.
  11. Маренникова С.С., Степанова Л.Г., Носик М.Н. и др. О штаммовых особенностях циркулирующего в СССР ВИЧ-1 по данным изучения его свойств в клеточных культурах. Вопросы вирусологии. 1991; 5: 356–61.
  12. Носик М.Н., Рыжов К.А., Киселева И.А., Кравченко А.В., Покровский В.В. Биологические свойства штаммов вируса иммунодефицита человека, выделенных от ВИЧ-инфицированных лиц на территории России за 2009–2010 гг. ЗНиСО. 2011; 4: 31–4.
  13. Ryzhov K.A., Nosik M.N., Pokrovsky V.V. et al. The genetic diversity of human immunodeficency virus-1 circulating in the territory of Russia. In: Proceedings 5th IAS Conference on HIV Pathogenesis, Treatment and Prevention. 2009; July 19–22; Cape Town; South Africa: CDAO34.
  14. Ryzhov K.A., Nosik M.N., Kravtchenko A.V. et al. Epidemiological monitoring of HIV-infection in the territory of Russia. In: VIII Miedzynarodowej naukowi-praktycznej konferencji Dynamika naukowych Badan-2012. 2012; July 7–15; Przemysl; Poland; Nauka I studia; 8: 72–4.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2015 Nossik M.N., Kiseleva I.A., Bochkova M.S., Ryzhov K.A., Kravtchenko A.V., Pokrovsky V.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies