Study of the isolated bacteriophage ΦAb-Sр7 adsorption on the cell surface of the  Azospirillum brasilense  Sp7

O. I. Guliy; Гулий О. И.; O. A. Karavaeva; Караваева О. А.; V. A. Velikov; Великов В. А.; O. I. Sokolov; Соколов О. И.; S. A. Pavliy; Павлий С. А.; O. S. Larionova; Ларионова О. С.; A. M. Burov; Буров А. М.; O. V. Ignatov; Игнатов О. В.

doi:10.18821/0507-4088-2016-61-1-45-48

Study of the isolated bacteriophage ΦAb-Sр7 adsorption on the cell surface of the Azospirillum brasilense Sp7

Authors: Guliy O.I.¹^,2^,3, Karavaeva O.A.¹, Velikov V.A.¹^,4, Sokolov O.I.¹, Pavliy S.A.⁴, Larionova O.S.², Burov A.M.¹, Ignatov O.V.¹
Affiliations:
1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences
2. Saratov State Vavilov Agrarian University
3. Scientific Research Veterinary Institute
4. Saratov State University
Issue: Vol 61, No 1 (2016)
Pages: 45-48
Section: ORIGINAL RESEARCH
URL: https://virusjour.crie.ru/jour/article/view/305
DOI: https://doi.org/10.18821/0507-4088-2016-61-1-45-48
ID: 305

Cite item

Full Text

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

The bacteriophage ΦAb-Sр7 was isolated from the cells of the azospirillum brasilense sp7. The morphology, size of the gram-negative colonies, and range of lytic activity against other strains and species of the genus Azospirillum was tested. The isolated phage DNA was examined using electrophoretic and restriction analysis, and the size of the genome were established. The electron microscopy results show that the phage (capsid) has a strand-like form. The electron microscopy study of the bacteriophage ΦAb-Sр7 adsorption on the A. brasilense Sp7 bacterial surface was performed.

Keywords

Azospirillum brasilense, bacteriophage, DNA electrophoresis, electron microscopy, adsorption

About the authors

O. I. Guliy

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences; Saratov State Vavilov Agrarian University; Scientific Research Veterinary Institute

Author for correspondence.
Email: guliy_olga@mail.ru

Ol’ga Guliy, MD, PhD, DSc

410049, Saratov
410012, Saratov
410028, Saratov

Russian Federation

References

Chang J.T., Schmid M.F., Haase-Pettingell C., Weigele P.R., King J.A., Chiu W. Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection. J. Mol. Biol. 2010; 402(4): 731–40.
Уткин Д.В., Ерохин П.С., Осина Н.А., Конов Н.П. Оценка фаголизабельности штаммов холерных вибрионов с использованием атомно-силовой микроскопии. Известия Саратовского университета. Новая серия. Серия: Химия. Биология. Экология. 2013; 13 (3): 81–4.
Краевский С.В. Атомно-силовая микроскопия аффинных взаимодействий в микробиологии: Дисс. … канд. биол. наук. Оболенск; 2011.
Kang A.S., Barbas C.F., Janda K.D., Benkovic S.J., Lerner R.A. Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. Proc. Natl. Acad. Sci. U S A. 1991; 88(10): 4363–6.
Suttle C.A. Viruses in the sea. Nature. 2005; 437: 356–61.
Ewing B., Hillier L., Wendl M.C., Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res.1998; 8(3): 175–85.
Germida J.J., Khachatourians G.G. Transduction of Escherichia coli in soil. Can. J. Microbiol. 1988; 34(2): 190–3.
Boyer M., Haurat J., Samain S., Segurens B., Gavory F., González V. et al. Bacteriophage prevalence in the genus Azospirillum and analyses of the first genome sequence of an Azospirillum brasilense integrative phage. Appl. Environ. Microbiol. 2008; 74(3): 861–74.
Gerhardt P., ed. Manual of Methods for General Bacteriology. American Society for Microbiology, Washington, DC; 1981.
Martha R.J.Clokie, AndrewM.Kropinski, eds. Bacteriophages Methods and Protocols. Vol.1: Isolation, Characterization, and Interaction. Humana Press; 2008: 113–26.
Sambrook J., Fritsch D.F., Maniatis T. Molecular Сloning: A Laboratory Manual. 2nd ed. (v. 1–3). Cold Spring Harbor, New York, Cold Spring Harbor Lab. Press; 1989.
Boyer M., Haurat J., Samain S., Segurens B., Gavory F., González V. et al. Bacteriophage prevalence in the genus Azospirillum and analyses of the first genome sequence of an Azospirillum brasilense integrative phage. Appl. Environ. Microbiol. 2008; 74(3): 861–74.
Stengele I., Bross P., Garces X., Giray J., Rasched I. Dissection of functional domains in phage fd adsorption protein. Discrimination between attachment and penetration sites. J. Mol. Biol. 1990; 212(1): 143–9.
Jakes K.S., Davis N.G., Zinder N.D. A hybrid toxin from bacteriophage f1 attachment protein and colicin E3 has altered cell receptor specificity. J. Bacteriol. 1988; 170(9): 4231–8.
Russel M., Whirlow H., Sun T.P., Webster R.E. Low-frequency infection of F-bacteria by transducing particles of Filamentous bacteriophages. J. Bacteriol. 1988; 170(11): 5312–6.
Krebber C., Spada S., Desplancq D., Krebber A., Ge L., Pluckthun A. Selectively infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein–ligand interactions. J. Mol. Biol. 1997; 268(3): 607–18.

Supplementary files

Supplementary Files

Action

1. JATS XML

Download

Username
Password
Remember me

Forgot password?	Register

Username
Password
Remember me

Forgot password?	Register