Identification of the infectious pancreatic necrosis virus (IPNV) using the enzyme mmunoassay

Cover Page


Cite item

Full Text

Abstract

The infectious pancreatic necrosis (IPN) caused by a non-enveloped virus of the Birnaviridae family is one of the most important loss factors in the salmonid aquaculture. Virus isolation in the sensitive cell cultures has been approved in the Russian Federation as the diagnostic method for determination of IPNV antigen. This work gives the results of the development of the diagnostic test to reveal IPNV using the antigen-bound ELISA (sandwich ELISA). The developed test supplements a new diagnostic method and verifies some disputable results obtained with classical methods.

About the authors

E. A. Zavyalova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Author for correspondence.
Email: aquazeda@mail.ru

Elena Zavyalova, Candidate of biological Sciences

109428, Moscow

Россия

M. I. Gulyukin

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Россия

M. A. Carpova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Россия

P. D. Bogdanova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Россия

A. E. Droshnev

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Россия

References

  1. Johansen L.H., Sommer A.I. Infectious pancreatic necrosis virus infection in Atlantic salmon Salmo salar post-smolt affects the outcome of secondary infectiouns with infectious salmon anaemia virus or Vibrio salmonicida. Dis. Aquat. Organ. 2001; 47(2): 109-17.
  2. Pedersen T., Skjesol A., Jørgensen J.B. VP3, a structural protein of infectious pancreatic necrosis virus, interact with RNA-depended RNA polymerase VP1 and with double-stranded RNA. J. Virol. 2007; 81(12): 6652–63.
  3. OIE Manual of diagnostic tests for aquatic animals. 5th edition. 2006.
  4. Evensen Ø., Skjelstad B., Rimstad E., Brun E., Johansen L.H., Stagg R. et al. IPN in salmonids. A review. The Fisheriers and Aquaculture Industries Research Fund (FHF); 2003.
  5. Гулюкин М.И., Завьялова Е.А., Дрошнев А.Е., Коломыцев С.А. Анализ эпизоотической ситуации по болезням рыб в России (2001–2011 гг.). Ветеринария. 2011; 8: 3–7.
  6. Досон Р., Эллиот Д., Эллиот У., Джонс К. Справочник биохимика. М.: Издательство «Мир»; 1991.
  7. Северин С.Е., Соловьева Г.А., ред. Практикум по биохимии. М.: Издaтельство МГУ; 1989.
  8. Wilson M.B., Nakane P.K. Recent development in the periodate method of conjugating horseradish peroxides (HRPO) to antibodies. In: Knapp W., Holubar K., Wick G., eds. Immunofluo-Rescence and Related Staining Techniques. Vienna, Austria: Elsevier/North-Holland; 1978; 215–44.
  9. Van der Marel P., Snyder D.B., Lutticken D. Antigenic characterization of IBDV field isolates by their reactivity with a panel of monoclonal antibodies. Dtsch. Tierarztl. Wochenschr. 1990; 97(2): 81–3.
  10. Остерман Л.А. Хроматография белков и нуклеиновых кислот. М.: Издательство «Наука»; 1985.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Zavyalova E.A., Gulyukin M.I., Carpova M.A., Bogdanova P.D., Droshnev A.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies