Identification of the infectious pancreatic necrosis virus (IPNV) using the enzyme mmunoassay

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Abstract

The infectious pancreatic necrosis (IPN) caused by a non-enveloped virus of the Birnaviridae family is one of the most important loss factors in the salmonid aquaculture. Virus isolation in the sensitive cell cultures has been approved in the Russian Federation as the diagnostic method for determination of IPNV antigen. This work gives the results of the development of the diagnostic test to reveal IPNV using the antigen-bound ELISA (sandwich ELISA). The developed test supplements a new diagnostic method and verifies some disputable results obtained with classical methods.

About the authors

E. A. Zavyalova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Author for correspondence.
Email: aquazeda@mail.ru

Elena Zavyalova, Candidate of biological Sciences

109428, Moscow

Russian Federation

M. I. Gulyukin

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Russian Federation

M. A. Carpova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Russian Federation

P. D. Bogdanova

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Russian Federation

A. E. Droshnev

Ya.R. Kovalenko All-Russian Institute of Experimental Veterinary Medicine

Email: fake@neicon.ru
109428, Moscow Russian Federation

References

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Copyright (c) 2016 Zavyalova E.A., Gulyukin M.I., Carpova M.A., Bogdanova P.D., Droshnev A.E.

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