Avian recombinant virus H5N1 influenza (A/Vietnam/1203/04) and its escape-mutant m13(13) induce early signaling reactions of the immunity in human lymphocytes

Cover Page


Cite item

Full Text

Abstract

The innate immune receptors TLR4, TLR7, TLR8, and RIG1 recognized the structures of the influenza viruses in human lymphocytes and were activated by the recombinant avian influenza virus A/Vietnam/1203/04 and its escape-mutant m13(13) during early period of interaction. The stimulated levels are not connected with viral reproduction. Donor cells with the low constitutive immune receptors gene expression levels showed higher stimulation. Inflammation virus effects resulted in increasing production of TNF-alpha and IFN-gamma by lymphocytes. Signaling gene reactions of the parent and mutant viruses endosomal as well as cytoplasmic receptors are very similar. The mutant virus A/Vietnam/1203/04 (HA S145F) stimulated an increase in the transcription level of the membrane receptor gene TLR4 and a decrease in the level of activation of TNF-alpha gene. Further studies of natural influenza virus isolates are necessary to estimate the role of HA antigenic changes on immune reactions in humans.

About the authors

T. M. Sokolova

D.I. Ivanovsky Institute of Virology “Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya”

Author for correspondence.
Email: tmsokolovavir@mail.ru

Tatyana Sokolova, Doctor of Biological Sciences, Academician of the Russian Academy of natura Sciences

123098, Moscow

Россия

V. V. Poloskov

D.I. Ivanovsky Institute of Virology “Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya”

Email: fake@neicon.ru
123098, Moscow Россия

A. N. Shuvalov

D.I. Ivanovsky Institute of Virology “Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya”

Email: fake@neicon.ru
123098, Moscow Россия

I. A. Rudneva

D.I. Ivanovsky Institute of Virology “Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya”

Email: fake@neicon.ru
123098, Moscow Россия

F. I. Ershov

D.I. Ivanovsky Institute of Virology “Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya”

Email: fake@neicon.ru
123098, Moscow Россия

References

  1. Webster R.G., Govorkova E.A. H5N1 influenza-containing evolution and spread N. Engl. J. Med. 2006; 355: 2174–7.
  2. Kaverin N.V., Rudneva I.A., Ilyushina N.A., Varich N.L., Lipatov A.S., Smirnov Y.A. et al. Structure of antigenic sites on the hemagglutinin molecule of H5 influenza virus and phenotypic variation of escape mutants. J. Gen.Virol. 2002; 83(Pt. 10): 2497–505.
  3. Stevens J., Blixt O., Tumpey T.M., Taubenberger J.K., Paulson J.C., Wilson I.A. Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus. Science. 2006; 312(5772): 404–10.
  4. Kaverin N.V., Rudneva I.A., Govorkova E.A., Timofeeva T.A., Shilov A.A., Kochergin-Nikitsky K.S. et al. Epitope mapping of the hemagglutinin molecule of highly pathogenic H5N1 influenza virus by using monoclonal antibodies. J. Virol. 2007; 81(23): 12911–7.
  5. Ramos I., Bernal-Rubio D., Durham N., Belicha-Villanueva A., Lowen A.C., Steel J. et al. Effects of receptor binding specificity of avian influenza virus on the human innate immune response. J. Virol. 2011; 85(9): 4421–31.
  6. Hou W., Gibbs J.S., Lu X., Brooke C.B., Roy D., Modlin R.L. et al. Viral infection triggers rapid differentiation of human blood monocytes into dendritic cells. Blood. 2012; 119(13): 3128-31.
  7. Pang I.K., Pillai P.S., Iwasaki A. Efficient influenza virus replication in respiratory tract requires signals from TLR3 and RIG1. Proc. Natl. Acad. Sci. U S A. 2013; 110(34): 13910–5.
  8. Соколова Т.М. Иммунное узнавание вирусных нуклеиновых кислот приводит к индукции интерферонов и воспалительных цитокинов. В кн.: Ершов Ф.И., ред. Сборник научных трудов «Интерферон-2011» . М.: НИИЭМ им. Н.Ф. Гамалеи МЗ РФ; 2012: 52–62.
  9. Kawai T., Akira S. Toll-like receptor and RIG1-like receptor signaling. Ann. NY Acad. Sci. 2008; 1143: 1–20.
  10. Lester S.N., Li K. Toll-like receptors in antiviral innate immunity. J. Mol. Biol. Avaiable at: http://dx.dos.org/10.1016.jmb.2013.11.024.
  11. Saha B., Prasanna S.J., Chandrasekar B., Nandi D. Gene modulation and immunoregulatory roles of interferon gamma. Cytokine. 2010; 50(1): 1–14.
  12. Соколова Т.М., Шувалов А.Н. Подавление рекомбинантным альфа-2-интерфероном репродукции вируса Карельской лихорадки в клетках крови человека. Вопросы вирусологии. 2011; 57(2): 27–31.
  13. Соколова Т.М., Шувалов А.Н. Телков М.В., Колодяжная Л.В., Ершов Ф.И. Препарат «Ридостин» индуцирует транскрипцию широкого спектра генов системы интерферона в клетках человека. Бюллетень экспериментальной биологии и медицины. 2013; 156(8): 179–82.
  14. Шувалов А.Н., Соколова Т.М., Шаповал И.М., Ершов Ф.И. Модуляция клеточных генов препаратом Иммуномакс: активация генов интерферонов и интерлейкинов. Иммунология. 2014; 35(1): 16–20.
  15. Cao W., Taylor A.K., Biber R.E., Davis W.G., Kim J.H., Reber A.J. et al. Rapid differentiation of monocytes into type I IFN-producing myeloid dendritic cells as an antiviral strategy against influenza virus infection. J. Immunol. 2012; 189(5): 2757–65.
  16. Cheng X., Xu Q., Song E., Yang C.F., Kemble G., Jin H. The hemagglutinin protein of influenza A/Vietnam/1203/2004(H5N1) control to hyperinduction of proinflamatory cytokines in human epithelial cells.Virology. 2010; 406(1): 28–36.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Sokolova T.M., Poloskov V.V., Shuvalov A.N., Rudneva I.A., Ershov F.I.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies