Vaccines against avian influenza in poultry

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The review presents the latest data about the types of vaccines against avian influenza that are used in current medical practice or are under development. Inactivated whole virion vaccines, live vector vaccines, as well as experimental vaccines developed using genetic engineering techniques (e.g. subunit vaccines, VLP vaccines, DNA vaccines) were considered. The efficiency of influenza reverse genetic technology for the development of prototype vaccine strains was noted.

About the authors

L. V. Kostina

Federal Research Centre of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Author for correspondence.
Russian Federation

A. D. Zaberezhnyy

Federal Research Centre of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya; Y.R. Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary

Russian Federation

T. V. Grebennikova

Federal Research Centre of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Russian Federation

N. V. Antipova

Peoples' Friendship University of Russia

Russian Federation

T. I. Aliper

Federal Research Centre of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya; Y.R. Kovalenko All-Russian Scientific Research Institute of Experimental Veterinary

Russian Federation

E. A. Nepoklonov

Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor)

Russian Federation


  1. Li C., Bu Z., Chen H. Avian influenza vaccines against H5N1 ‘bird flu’. Trends Biotechnol. 2014; 32 (3): 147-56.
  2. Приказ Минсельхоза РФ № 90 “Об утверждении правил по борьбе с гриппом птиц”. М.; 2006.
  3. Von Dobschuetz S., Siembieda J., Kim M., Pinto J., Newman S. Highly Pathogenic Avian Influenza. EMPRES Transboundary Animal Diseases Bulletin. 2011; (37): 21-9.
  4. Swayne D.E, Kapczynski D. Vaccines, vaccination and immunology for avian influenza viruses in poultry. In: Swayne D.E., ed. Avian Influenza. Ames, Iowa: Blackwell Publishers; 2008: 407-52.
  5. Swayne D.E., Pavade G., Hamilton K., Vallat B., Miyagishima K. Assessment of national strategies for control of high-pathogenicity avian influenza and low-pathogenicity notifiable avian influenza in poultry, with emphasis on vaccinesand vaccination. Rev. Sci. Tech. 2011; 30 (3): 839-70.
  6. Swayne D.E. Principles for vaccine protection in chickens and domestic waterfowl against avian influenza: Emphasis on Asian H5N1 high pathogenicity avian influenza. Ann. N. Y. Acad. Sci. 2006; 1081: 174-81.
  7. Council for Agricultural Science and Technology (CAST). Avian Influenza Vaccination: A Commentary Focusing on H5N1 High Pathogenicity Avian Influenza. CAST Commentary QTA2007-3. Ames, Iowa: CAST; 2007.
  8. Swayne D.E. Avian influenza vaccines and therapies for poultry. Comp. Immunol. Microbiol. Infect. Dis. 2009; 32 (4): 351-63.
  9. Avian influenza vaccine producers and suppliers for poultry (June 2009). Available at:
  10. Львов Д.К., Алипер Т.И., Дерябин П.Г., Забережный А.Д., Гребенникова Т.В., Сергеев В.А. и др. Вакцина против гриппа птиц инактивированная эмульгированная ФЛУ ПРОТЕКТ Н5 и способ профилактики гриппа птиц. Патент РФ № 2350350; 2009.
  11. Peyre M., Fusheng G., Desvaux S., Roger F. Avian influenza vaccines: a practical review in relation to their application in the field with a focus on the Asian experience. Epidemiol. Infect. 2009; 137 (1): 1-21.
  12. FAD PReP/NAHEMS Guidelines: Vaccination for contagiousdiseases/Appendix C: Vaccination for high pathogenicity avian influenza (August 2015). Available at:
  13. Bublot M., Pritchard N., Swayne D.E., Selleck P., Karaca K., Suarez D.L. et al. Development and use of fowlpox vectored vaccines for avian influenza. Ann. N. Y. Acad. Sci. 2006; 1081: 193-201.
  14. Suarez D.L., Schultz-Cherr S. Immunology of avian influenza virus: a review. Dev. Comp. Immunol. 2000; 24 (2-3): 269-83.
  15. Swayne D.E., Kapczynski D. Strategies and challenges for eliciting immunity against avian influenza virus in birds. Immunol. Rev. 2008; 225: 314-31.
  16. Neumann G., Watanabe T., Ito H., Watanabe S., Goto H., Gao P. et al. Generation of influenza A viruses entirely from cloned cDNAs. Proc. Natl. Acad. Sci. USA. 1999; 96 (16): 9345-50.
  17. Fodor E., Devenish L., Engelhardt O.G., Palese P., Brownlee G.G., Garcia-Sastre A. Rescue of influenza A virus from recombinant DNA. J. Virol. 1999; 73 (11): 9679-82.
  18. Hoffmann E., Neumann G., Kawaoka Y., Hobom G., Webster R.G. A DNA transfection system for generation of influenza A virus from eight plasmids. Proc. Natl. Acad. Sci. USA. 2000; 97 (11): 6108-13.
  19. Song H., Nieto G., Perez D. A new generation of modified liveattenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates. J. Virol. 2007; 81 (17): 9238-48.
  20. Steel J., Lowen A.C., Pena L., Angel M., Solórzano A., Albrecht R. et al. Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza. J. Virol. 2009; 83 (4): 1742-53.
  21. Watanabe T., Watanabe S., Hyun Kim J., Hatta M., Kawaoka Y. Novel Approach to the Development of Effective H5N1 Influenza A Virus Vaccines: Use of M2 Cytoplasmic Tail Mutants. J. Virol. 2008; 82 (5): 2486-92.
  22. Uchida Y., Takemae N., Saito T. Application of reverse genetics for producing attenuated vaccine strains against highly pathogenic avian influenza viruses. J. Vet. Med. Sci. 2014; 76 (8): 1111-7.
  23. Chambers T.M., Quinlivan M., Sturgill T., Cullinane A., Horohov D.W., Zamarin D. et al. Influenza a viruses with truncated NS1 as modified live virus vaccines: Pilot studies of safety and efficacy in horses. Equine Vet. J. 2009; 41 (1): 87-92.
  24. Richt J.A., Lekcharoensuk P., Lager K.M., Vincent A.L., Loiacono C.M., Janke B.H. et al. Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine. J. Virol. 2006; 80 (22): 11 009-18.
  25. Забережный А.Д., Гребенникова Т.В., Воркунова Г.К., Южаков А.Г., Костина Л.В., Норкина С.Н. и др. Получение нового штамма-реассортанта вируса гриппа А/Н5N1 методом обратной генетики и анализ его биологических свойств. Вопросы вирусологии. 2014; (6): 23-7.
  26. Webster R.G., Webby R.J., Hoffmann E., Rodenberg J., Kumar M., Chu H.J. et al. The immunogenicity and efficacy against H5N1 challenge of reverse genetics-derived H5N3 influenza vaccine in ducks and chickens. Virology. 2006; 351 (2): 303-11.
  27. Tian G., Zhang S., Li Y., Bu Z., Liu P., Zhou J. et al. Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics. Virology. 2005; 341 (1): 153-62.
  28. Chen H. Avian influenza vaccination: the experience in China. Rev. Sci. Tech. 2009; 28 (1): 267-74.
  29. Сергеева М.В., Крохин А., Матросович М., Матросович Т., Волшек М., Киселев О.И. и др. Влияние конформационной стабильности гемагглютинина вируса гриппа на качество инактивированных вакцин H5N1. Microbiology Independent Research Journal. 2014; (1): 1-11.
  30. van den Berg T., Lambrecht B., Marché S., Steensels M., Van Borm S., Bublot M. Influenza vaccines and vaccination strategies in birds. Comp. Immunol. Microbiol. Infect. Dis. 2008; 31 (2-3): 121-65.
  31. Spackman E., Swayne D.E. Vaccination of gallinaceous poultry for H5N1 highly pathogenic avian influenza: Current questions and new technology. Virus Res. 2013; 178 (1): 121-32.
  32. Taylor J., Weinberg R., Kawaoka Y., Webster R.G., Paoletti E. Protective immunity against avian influenza induced by a fowlpox virus recombinant. Vaccine. 1988; 6 (6): 504-8.
  33. Qiao C., Jiang Y., Tian G., Wang X., Li C., Xin X. et al. Recombinant fowlpox virus vector-based vaccine completely protects chickens from H5N1 avian influenza virus. Antiviral Res. 2009; 81 (3): 234-8.
  34. Qiao C.L., Yu K.Z., Jiang Y.P., Jia Y.Q., Tian G.B., Liu M. et al. Protection of chickens against highly lethal H5N1 and H7N1 avian influenza viruses with a recombinant fowlpox virus co-expressing H5 haemagglutinin and N1 neuraminidase genes. Avian Pathol. 2003; 32 (1): 25-32.
  35. Swayne D.E., Beck J.R., Kinney N. Failure of a recombinant fowl poxvirus vaccine containing an avian influenza hemagglutinin gene to provide consistent protection against influenza in chickens preimmunized with a fowl pox vaccine. Avian Dis. 2000; 44 (1): 132-7.
  36. Swayne D.E., Garcia M., Beck J.R., Kinney N., Suarez D.L. Protection against diverse highly pathogenic H5 avian influenza viruses in chickens immunized with a recombinant fowlpox vaccine containing an H5 avian influenza hemagglutinin gene insert. Vaccine. 2000; 18 (11-12): 1088-95.
  37. Swayne D.E., Perdue M.L., Beck J.R., Garcia M., Suarez D.L. Vaccines protect chickens against H5 highly pathogenic avian influenza in the face of genetic changes in field viruses over multiple years. Vet. Microbiol. 2000; 74 (1-2): 165-72.
  38. Webster R.G., Taylor J., Pearson J., Rivera E., Paoletti E. Immunity to Mexican H5N2 avian influenza viruses induced by a fowlpox-H5 recombinant. Avian Dis. 1996; 40 (2): 461-5.
  39. Boyle D.B., Selleck P., Heine H.G. Vaccinating chickens against avianinfluenza with fowlpox recombinants expressing the H7 haemagglutinin. Aust. Vet. J. 2000; 78 (1): 44-8.
  40. Beard C.W., Schnitzlein W.M., Tripathy D.N. Effect of route of administrationon the efficacy of a recombinant fowlpox virus against H5N2 avian influenza. Avian Dis. 1992; 36 (4): 1052-5.
  41. Steensels M., Bublot M., Van Borm S., De Vriese J., Lambrecht B., Richard-Mazet A. et al. Prime-boost vaccination with a fowlpox vector and an inactivated avian influenza vaccine is highly immunogenic in Pekin ducks challenged with Asian H5N1 HPAI. Vaccine. 2009; 27 (5): 646-54.
  42. Swayne D.E., Beck J.R., Mickle T.R. Efficacy of recombinant fowl poxvirus vaccine in protecting chickens against a highly pathogenic Mexican-origin H5N2 avian influenza virus. Avian Dis. 1997; 41 (4): 910-22.
  43. Qiao C., Yu K., Jiang Y., Li C., Tian G., Wang X. et al. Development of a recombinant fowlpox virus vector-based vaccine of H5N1 subtype avian influenza. Dev. Biol. (Basel). 2006; 124: 127-32.
  44. Lozano B., Soto E., Sarfati D., Castro F., Gay M., Antillon A. A novel engineered live viral vaccine against Newcastle disease and avian influenza subtype H5. In: Capua I., ed. Proceedings of the FAO/OIE Conference on Vaccination: a Tool for the Control of Avian Influenza. Verona, Italy; 2007.
  45. Swayne D.E., Suarez D.L., Schultz-Cherry S., Tumpey T.M., King D.J., Nakaya T. et al. Recombinant paramyxovirus type 1-avian influenza-H7 virus as a vaccine for protection of chickens against influenza and Newcastle disease. Avian Dis. 2003; 47 (Suppl.3): 1047-50.
  46. Park M.S., Steel J., García-Sastre A., Swayne D., Palese P. Engineeredviral vaccine constructs with dual specificity: avian influenza and Newcastle disease. Proc. Natl. Acad. Sci. USA. 2006; 103 (21): 8203-8.
  47. Ge J., Deng G., Wen Z., Tian G., Wang Y., Shi J. et. al. Newcastle disease virus-based live attenuated vaccine completely protects chickens and mice from lethal challenge of homologous and heterologous H5N1 avian influenza viruses. J. Virol. 2007; 81 (1): 150-8.
  48. Nayak B., Rout S.N., Kumar S., Khalil M.S., Fouda M.M., Ahmed L.E. et al. Immunization of chickens with Newcastle disease virus expressing H5 hemagglutinin protects against highly pathogenic H5N1 avian influenza viruses. 2009. Available at:
  49. Liu Q., Mena I., Ma J., Bawa B., Krammer F., Lyoo Y.S. et al. Newcastle Disease Virus-Vectored H7 and H5 Live Vaccines Protect Chickens from Challenge with H7N9 or H5N1 Avian Influenza Viruses. J. Virol. 2015; 89 (14): 7401-8.
  50. Lee D.H., Park J.K., Song C.S. Progress and hurdles in the development of influenza virus-like particle vaccines for veterinary use. Clin. Exp. Vaccine Res. 2014; 3 (2): 133-9.
  51. Wang B.Z., Quan F.S., Kang S.M., Bozja J., Skountzou I., Compans R.W. Incorporation of membrane-anchored flagellin into influenza virus-like particles enhances the breadth of immune responses. J. Virol. 2008; 82 (23): 11 813-23.
  52. Noh J.Y., Park J.K., Lee D.H., Yuk S.S., Kwon J.H., Lee S.W. et al. Chimeric Bivalent Virus-Like Particle Vaccine for H5N1 HPAI and ND Confers Protection against a Lethal Challenge in Chickens and Allows a Strategy of Differentiating Infected from Vaccinated Animals (DIVA). PLoS One. 2016; 11 (9): e0162946.
  53. Петухова Н.В., Иванов П.А., Мигунов А.И. Вирусоподобные частицы - новая стратегия для создания противогриппозных вакцин. Вопросы вирусологии. 2013; (2): 10-4.
  54. Qiu M., Fang F., Chen Y., Wang H., Chen Q., Chang H. et al. Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin- or neuraminidase-expressing DNA in BALB/c mice. Biochem. Biophys. Res. Commun. 2006; 343 (4): 1124-31.
  55. Ljungberg K., Kolmskog C., Wahren B., van Amerongen G., Baars M., Osterhaus A. et al. DNA vaccination of ferrets with chimeric influenza A virus hemagglutinin (H3) genes. Vaccine. 2002; 20 (16): 2045-52.
  56. Gall-Reculé G. Le, Cherbonnel M., Pelotte N., Blanchard P., Morin Y., Jestin V. Importance of a prime-boost DNA/protein vaccination to protect chickens against low-pathogenic H7 avian influenza infection. Avian Dis. 2007; 51 (Suppl. 1): 490-4.
  57. Cherbonnel M., Rousset J., Jestin V. Strategies to improve protection against low-pathogenicity H7 avian influenza virus infection using DNA vaccines. Avian Dis. 2003; 47 (Suppl. 3): 1181-6.
  58. Ullah S., Riaz N., Umar S., Shah M.A.A. DNA Vaccines against Avian Influenza: current research and future prospects. World Poultry Sci. J. 2013; 69 (1): 125-34.
  59. Neumann G., Horimoto T., Kawaoka Y. Reverse Genetics of Influenza Viruses - Applications in Research and Vaccine Design. In: Klenk H.D., Matrosovich M.N., Stech J., eds. Avian Influenza. Monographs in virology. Vol. 27. Basel: Karger; 2008: 118-33.

Copyright (c) 2017 Kostina L.V., Zaberezhnyy A.D., Grebennikova T.V., Antipova N.V., Aliper T.I., Nepoklonov E.A.

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