Monoclonal antibodies with high virus-neutralizing activity against pandemic influenza virus A/IIV-Moscow/01/2009 (H1N1)swl


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Abstract

The authors have obtained a panel of 7 monoclonal antibodies (MAbs) against pandemic influenza virus A/IIV-Moscow/01/2009 (H1N1)swl isolated in Russia. One MAb is directed to a NP protein linear epitope and interacts with all the influenza A viruses under study. Six other MAbs are directed to H1 hemagglutinin conformation-dependent determinants and detect homologous virus in the hemagglutination-inhibition test, enzyme immunoassay, immunofluorescence and virus neutralization tests. MAbs differentiate pandemic influenza viruses A(H1N1)swl from seasonal influenza A(H1N1), A(H3N2), and B viruses. The high neutralizing activity of MAbs permits their use to study the fine antigen structure of influenza virus hemagglutinin and to differentiate the A(H1N1) pandemic influenza viruses and offers promise for obtaining humanized antibodies in order to make specific prevention and treatment of influenza.

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Copyright (c) 2011 Klimova R.R., Masalova O.V., Burtseva E.I., Chichev E.V., Lesnova E.I., Oskerko T.A., Mukasheva E.A., Rudneva I.A., L'vov D.K., Kushch A.A., Klimova R.R., Masalova O.V., Burtseva E.I., Chichev E.V., Lesnova E.I., Oskerko T.A., Mukasheva E.A., Rudneva I.A., Lvov D.K., Kushch A.A.

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