Email this article Genotyping of varicella zoster virus strains isolated in Mongolia
- Issue: Vol 55, No 5 (2010)
- Pages: 40-43
- Section: Articles
- URL: https://virusjour.crie.ru/jour/article/view/12056
- ID: 12056
Cite item
Full Text
Abstract
This study analyzed 50 varicella zoster virus (VZV) samples collected during 2004 to 2007 from patients with VZV infection, who were treated at the National Center of Communicable Diseases, Ulan-Bator, Mongolia. The method based on amplification of specific DNA fragments of the ORF21, ORF22, and ORF50 genes was used, followed by the sequencing and detection of the status of characteristic point mutations in these fragments. The results indicated that the collected samples belonged to genotypes J (62%), M1 (18%), E1 (12%), E2 (4%), and M2 (4%). Moreover, restriction endonuclease polymorphism in ORF 62 for the cleavage site SmaI and MspI, in ORF 38 and ORF 54 for the cleavage site PstI and BglI were analyzed. All the samples were Sma- Msp-. All samples with genotype E were Pst+ Bgl-; all samples with genotype M1 and M2 were Pst+ Bgl+. Out of 31 samples with genotype J, 29 and 2 were Pst+ Bgl+ and Pst- Bgl+, respectively. The study could identify the genotypes of VZV circulating in Mongolia and confirmed the absence of mutations characteristic for the vaccine strain.
Keywords
References
- Arvin A. M. Varicella-zoster virus: overview and clinical manifestations // Semin. Dermatol. 1996. - Vol. 15, N 2. - Suppl. - P. 4-7.
- LaRussa P. et al. Viral strain identification in varicella vaccinees with disseminated rashes // Pediatr. Infect. Dis. J. - 2000. - Vol. 19, N 11. - P. 1037-1039.
- LaRussa P. S., Gershon A. A. Biologic and geographic differences between vaccine and clinical varicella-zoster virus isolates // Arch. Virol. - 2001. - Vol. 17. - Suppl. - P. 41-48.
- Liu J. et al. Genotyping of clinical varicella-zoster virus isolates collected in China // J. Clin. Microbiol. - 2009. - Vol. 47, N 5. - P. 1418-1423.
- Loparev V. N. et al. Improved identification and differentiation of varicella-zoster virus (VZV) wild-type strains and an attenuated varicella vaccine strain using a VZV open reading frame 62-based PCR // J. Clin. Microbiol. - 2000. - Vol. 38, N 9. - P. 3156-3160.
- Loparev V. N. et al. Global identification of three major genotypes of varicella-zoster virus: longitudinal clustering and strategies for genotyping // J. Virol. 2004. - Vol. 78, N 15. - P. 8349-8358.
- Loparev. V. N. et al. Identification of five major and two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand // J. Virol. - 2007. - Vol. 81, N 23. - P. 12758-12765.
- Loparev V. N. et al. Distribution of varicella-zoster virus (VZV) wild-type genotypes in northern and southern Europe: evidence for high conservation of circulating genotypes // Virology. - 2009. - Vol. 383, N 2. - P. 216-225.
- Norberg P. et al. Complete-genome phylogenetic approach to varicella-zoster virus evolution: genetic divergence and evidence for recombination // J. Virol. - 2006. - Vol. 80, N 19. - P. 9569-9576.
- Peters G. A. et al. A full-genome phylogenetic analysis of varicella-zoster virus reveals a novel origin of replication-based genotyping scheme and evidence of recombination between major circulating clades // J. Virol. - 2006. - Vol. 80, N 19. - P. 9850-9860.
- Sergeev N. et al. New mosaic subgenotype of varicella-zoster virus in the USA: VZV detection and genotyping by oligonucleotide-microarray // J. Virol. Meth. - 2006. - Vol. 136, N 1- 2. - Р. 8-16.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).