Email this article A real-time polymerase chain reaction-based test system for quantitation of Gumboro disease virus
- Issue: Vol 55, No 3 (2010)
- Pages: 47-49
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.06.2010
- URL: https://virusjour.crie.ru/jour/article/view/12044
- ID: 12044
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Abstract
A real-time polymerase chain reaction-based test system for quantitation of infectious bursal disease (Gumboro disease) virus was developed. The reaction parameters were analyzed, which affected the linear relationship of a C1 depentanizer to the quantity of cDNA. The use of specific primers for reverse transcription was shown to have some advantage over that of random hexanucleotides.
References
- Bayliss C. D., Spies U., Shaw K., Peters R. W. et al. A comparison of the sequence of segment A of four infection bursal disease virus strains and identification of a variable region in the VP2 // J. Gen. Virol. - 1990. - Vol. 71. - P. 1303-1312.
- Brown M. D., Skiner M. A. Coding sequences of both genome segments of a European very virulent infectious bursal disease virus // Virus Res. - 1996 - Vol. 40, N 1. - P. 1-15.
- Henry C. W., Brewer R. N., Edgar S. A. Studies on infectious bursal disease in chickens. 2. Scoring microscopic lesions in the bursa of fabricius, thymus, spleen, and kidney in gnotobiotic and battery reared white leghorns experimentally infected with infectious bursal disease virus // Poult. Sci. - 1980. - Vol. 59. - P. 1006-1017.
- Kibenge F. S. B., Dhillon A. S., Russell R. G. Biochemistry and immunology of infectious bursal disease virus // J. Gen. Virol. - 1988. - Vol. 69. - P. 1757-1775.
- Lin H.-J., Giambrone J. J., Dormitorio T. Detection of genetic variations in serotype 1 isolates of infectious bursal disease virus using polymerase chain reaction and restriction and nuclease analysis // J. Virol. Meth. - 1994. - Vol. 48. - P. 281-291.
- Moody A., Sellers S., Bumstead N. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR // J. Virol. Meth. - 2000. - Vol. 85. - P. 55-64.
- Petkov D., Linnemann E., Kapczynski D. R., Sellers H. S. Full-length sequence analysis of four IBDV strains with different pathogenicities // Virus. Genes. - 2007. - Vol. 34, N 3. - P. 315-326.
- Proffitt J. M., Bastin D. A., Lehrbach P. R. Sequence analysis of Australian infectious bursal disease viruses // Aust. Vet. J. - 1999. - Vol.77. - P. 186-188.
- Wu C. C., Lin T. L., Zhang H. G. et al. Molecular detection of infectious bursal disease virus by polymerase chain reaction // Avian. Dis. - 1992. - Vol. 36. - P. 221-226.
- Zierenberg K., Raue R., Müller H. Rapid identification of very virulent strains of infectious bursal disease virus by reverse transcriptase-polymerase chain reaction combined with restriction enzyme analysis // Avian Pathol. - 2001. - Vol. 30. - P. 55-62.
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).