Problems of VirologyProblems of VirologyВопросы вирусологии0507-40882411-2097Central Research Institute for Epidemiology12044ArticlesСтатьиA real-time polymerase chain reaction-based test system for quantitation of Gumboro disease virusТест-система на основе ПЦР в реальном времени для количественного определения вируса болезни Гамборо15062010553NO3 (2010)№3 (2010)474909062023Copyright ©; 2010, Pogoda A.A., Potekhina M.A., Pogoda A.A., Potekhina M.A.Copyright ©; 2010, Погода А.А., Потехина М.А., Pogoda A.A., Potekhina M.A.2010Pogoda A.A., Potekhina M.A., Pogoda A.A., Potekhina M.A.Погода А.А., Потехина М.А., Pogoda A.A., Potekhina M.A.https://creativecommons.org/licenses/by/4.0https://virusjour.crie.ru/jour/article/view/12044

A real-time polymerase chain reaction-based test system for quantitation of infectious bursal disease (Gumboro disease) virus was developed. The reaction parameters were analyzed, which affected the linear relationship of a C1 depentanizer to the quantity of cDNA. The use of specific primers for reverse transcription was shown to have some advantage over that of random hexanucleotides.

Разработана тест-система для количественной оценки вируса инфекционной бурсальной болезни (болезни Гамборо) на основе реал-тайм ПЦР. Проанализированы условия и диапазон линейной зависимости порогового цикла розжига от количества матрицы. Показано преимущество использования специфичных праймеров по сравнению со случайными гексануклеотидами при получении кДНК.

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