Email this article The genetic variabilitynof the protease-encoding region in HIV-1 A isolatesand in CRF03 AB recombinants as observed in the CIS territory
- Issue: Vol 49, No 6 (2004)
- Pages: 4-10
- Section: Articles
- URL: https://virusjour.crie.ru/jour/article/view/11871
- ID: 11871
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Abstract
Protease-encoding nucleotide sequences of 27 HIV-1 variants isolated in Russia and other CIS
countries from seropositive intravenous drug-users were analyzed. None of the above persons did ever take antiretroviral drugs.
The nucleotide sequences were shown to belong to subtypes A and to be have a high degree of genetic
homogeneity (0.00-3.23; mean - 1.38 ± 0.79). No isolates contained any primary mutations of resistance to protease inhibitors.
At the same time, above one half of the isolates bore the V771 substitution, which, according to published
data, is the secondary mutation of resistance that conditions a higher resistance to Nelfinavir. Moreover,
the substitution was associated with 2 synonymous mutations in triplets 31 and 78, which denotes a single origin for all
V771 variants.
countries from seropositive intravenous drug-users were analyzed. None of the above persons did ever take antiretroviral drugs.
The nucleotide sequences were shown to belong to subtypes A and to be have a high degree of genetic
homogeneity (0.00-3.23; mean - 1.38 ± 0.79). No isolates contained any primary mutations of resistance to protease inhibitors.
At the same time, above one half of the isolates bore the V771 substitution, which, according to published
data, is the secondary mutation of resistance that conditions a higher resistance to Nelfinavir. Moreover,
the substitution was associated with 2 synonymous mutations in triplets 31 and 78, which denotes a single origin for all
V771 variants.
References
- Бобков А. Ф., Казеннова Е. В., Бобкова М. Р. и др. Молекулярно-генетическая характеристика ВИЧ-1 на территории России // Вестн. РАМН. - 2002. - № 8. - С. 40-42.
- ВИЧ-инфекция: клиника, диагностика и лечение / Покровский В. В., Ермак Т. Н., Беляева В. В., Юрин О. Г. - М., 2003. - С. 418-426.
- Ammaranond P., Cunningham P., Oelrichs R. et al. Rates of transmission of antiretroviral drug resistant strains of HIV-1 //J. Clin. Virol. -2003. - Vol. 26. - P. 153-161.
- Bobkov A., Kazennova E., Selimova L. et al. A sudden epidemic of HIV type 1 among injecting drug users in the former Soviet Union: Identification of the subtype A, subtype B, and novel gagA/envB recombinants // AIDS Res. Hum. Retrovirus. - 1998. - Vol. 14. - P. 669-676.
- Brenner B. G., Wainberg M. A. The role of antiretrovirals and drug resistance in vertical transmission of HIV-1 infection //Ann. N. Y. Acad. Sci. - 2000. - Vol. 918. - P. 9-15.
- Carrillo A., Stewart K. D., Norbeck D. W. et al. In vitro selection and characterization of human immunodeficiency virus type 1 variants with increased resistance to ABT-378, a novel protease inhibitor // J. Virol. - 1998. - Vol. 72. - P. 7532-7541.
- Delwart E. L., Shpaer E. G., Louwagie J. et al. Genetic relationships determined by a DNA heteroduplex mobility assay // Science. - 1993. - Vol. 262. - P. 1257-1261.
- Duwe S., Brunn M., Altmann D. et al. Frequency of genotypic and phenotypic drugresistant HIV-1 among therapynaTve patients of the German seroconverter study // J. Acquir. Immune Defic. Syndr. - 2001. - Vol. 26. - P. 266-273.
- Eelsenstein J. PHYLIP - phylogenetic interference package (version 3.2) // Cladistics. - 1989. - Vol. 5. - P. 164-166.
- Fonjungo P. N., Mpoudi E. N., Torimoro J. N. et al. Human immunodeficiency virus types 1 group M protease in Cameroon: genetic diversity and protease inhibitor mutational features // J. Clin. Microbiol. - 2002. - Vol. 40. - P. 837-845.
- Heyndickx L., Janssens W., Zekeng L. et al. Simplified strategy for detection of recombinant" human immunodeficiency virus type 1 group M isolates by gag/env heteroduplex mobility assay// J. Virol. - 2000. - Vol. 74. - P. 363-370.
- Liitsola K., Holm K., Bobkov A. An AB recombinant and its parental HIV type 1 strains in the area of the former Soviet Union: low requirements for sequence identity in recombination. UNAIDS Virus Isolation Network // AIDS Res. Hum. Retroviruses. - 2000. - Vol. 16. - P. 1047-1053.
- Patick A. K., Duran M., Cao Y. et al. Genotypic and phenotypic characterization of human immunodeficiency virus type 1 variants isolated from patients treated with the protease inhibitor Nelfinavir // Antimicrob. Agents Chemother. - 1998. - Vol. 42. - P. 2637-2644.
- Resch W., Hermann R., Parkin N. et al. Nelfinavir-resistant, Amprenavir-hypersusceptible strains of human immunodeficiency virus type 1 carrying an N88S mutation in protease have reduced infectivity, reduced replication capacity, and reduced fitness and process the gag polyprotein precursor aberrantly //J. Virol. - 2002. - Vol. 76. - P. 8659-8666.
- Servais J., Lambert C., Fontaine E. et al. Variant human immunodeficiency virus type 1 proteases and response to combination therapy including a protease inhibitor // Antimicrob. Agents Chemother. - 2001. - Vol. 45. - P. 893-900.
- Vazquez de Parga E., Thomson M. M., Rakhmanova A. et al. Sequence analysis of HIV-1 reverse transcriptase and protease genes and V3 region in a cohort of patients from the former Soviet Union // 14-th International AIDS Conference. - Barcelona, 2002. - Abstr. TuPeC4807.
- Walsh J. C., Pozniak A. L., Nelson M. R. et al. Virologic rebound on HAART in the context of low treatment adherence with a low prevalence of antiretroviral drug resistance // J. Acquir. Immune Defic. Syndr. - 2002. - Vol. 30. - P. 278-287.
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