Synthesis of recombinant proteins of the American and European viruses of the porcine reproductive and respiratory syndrome made in the baculovirus system and their antigen properties


Cite item

Full Text

Abstract

The baculovirus expression system was made use of to derive the recombinant nucleocapsid (N) proteins of the American and European virus types and of the porcine reproductive and respiratory syndrome (PRRS). The obtained products were purified by metal-affine chromatography and their specificity was confirmed in immunechemical reactions with reference monoclonal antibodies. The antigenic activity of recombinant proteins was studied by indirect immune enzyme assay (IEA) with porcine serum, which had been in advance characterized by the "HerdCheck" kit (IDEXX Co.). It was shown as possible to apply the derived recombinant antigens in determining, by indirect IEA, antibodies to the PRRS virus.

References

  1. Albina Е., Leforban Y., Baron T. et al. An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus // Ann. Rech. Vet. - 1992. - Vol. 23. - P. 167-176.
  2. Cavanagh D. Nidovirales: a new order comprising Coronaviridae and Arteriviridae // Arch. Virol. - 1997. - Vol. 142. - P. 629-633.
  3. Christopher-Hennings J., Nelson E. A., Nelson K. et al. Detection of porcine reproductive and respiratory syndrome virus in bolar semen by PCR // J. Clin. Microbiol. - 1995. - Vol. 33, N 7. - P. 1730-1734.
  4. Drew T. W., Lowing J. P., Yapp F. Variation in open reading frames 3, 4 and 7 among porcine reproductive and respiratory syndrome vims isolates in UK // Vet. Microbiol. - 1997. - Vol. 55. - P. 209-221.
  5. Houben S., Calebant P., Pensaert M. B. Comparative study of blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pig // J. Virol. Meth. - 1995. - Vol. 51. - P. 125-128.
  6. Kapur V., Elman M. R., Pawlovich T. M., Murtaugh M. P. Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the Midwestern United State // J. Gen. Virol. - 1996. - Vol. 77. - P. 1271-1276.
  7. Madrassi В., Wilson L., Mounir S., Dea S. Detection of porcine reproductive and respiratory syndrome vims and efficient differentiation between Canadian and European strains by reverse transcription and PCR amplification // J. Clin. Microbiol. - 1994. - Vol. 32. - P. 2197-2203.
  8. Meng X. L., Paul P. S., Halbur P. C., Lum M. A. Phylogenetic analyses of the putative M (ORF6) and N (ORF7) genes of porcine reproductive and respiratory syndrome vims (PRRSV): Implication for the existence of two genotypes of PRRSV in the USA and Europe // Arch. Viron. - 1995. - Vol. 140. - P. 745-755.
  9. Mengeling W. L., Lager K. M., Vorwald A. C. Diagnosis of porcine reproductive and respiratory syndrome // J. Vet. Diagn. Invest. - 1995. - Vol. 7. - P. 3-16.
  10. Meulenberg J. J. M., Petersen-den Besten A., de Kluyver E. P. et al. Characterization of proteins encoded by ORF2 to 7 of Lelystad virus // Virology. - 1995. - Vol. 206. - P. 155-163.
  11. Meulenberg J. J., Besten A. D., Kluyver E. D. et al. Molecular characterization of Lelystad vims // Vet. Microbiol. - 1997. - Vol. 55. - P. 197-202.
  12. Murtaugh M. P., Elam M. R., Kakach L. T. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad vims strains of the PRRS virus // Arch. Virol. - 1995. - Vol. 140. - P. 1451-1460.
  13. Paton D. J., Brown I. H., Scott A. C. et al. Isolation of a Lelystad vims-like agent from British pigs and scanning electronic microscopy of infected macrophages // Vet. Microbiol. - 1992. -Vol. 33. -P. 195-201.
  14. Snijder E. J., Meulenberg J. J. The molecular biology of arteriovimses // J. Gen. Virol. - 1998. - Vol. 79. - P. 961-979.
  15. Suarez P., Zardoya R., Martin M. J. et al. Phylogenetic relationship of European strain of porcine reproductive and respiratory syndrome virus (PRRSV) inferred from DNA sequences of putative ORF-5 and OFR-7 genes // Vims Res. - 1996. - Vol. 42. - P. 159-165.
  16. Wensvoort G., Terpstra C., Pol J. M. et al. Mystery swine disease in the Netherlands: the isolation of Lelystad vims // Vet. Quart. - 1991. - Vol. 13. - P. 121-130.
  17. Yang L., Yoon K.-J., Li Y. et al. Antigenic and genetic variations of the 15 kD nucleocapsid protein of porcine reproductive and respiratory syndrome vims isolates // Arch. Virol. - 1999. - Vol. 144. - P. 525-546.
  18. Yon L. J., Jo H. S., Christiansen W. T. et al. An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome vims in swine sera // J. Vet. Diagn. Invest. - 1992. - Vol. 4. - P. 14-147.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2004 Grebennikova T.V., Alexeev K.P., Gibadulin R.A., Musiyenko M.I., Tsibezov V.V., Bogdanova V.S., Zaberezhny A.D., Aliper T.I., Nepoklonov E.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies