Specific determination of flavioviruses by molecular hybridization with synthetic deoxyoligonucleotides probes


Cite item

Full Text

Abstract

Molecular probes were designed for the purpose of specific determination of flavioviruses transmitted by the ticks of tick-borne encephalitis (TBE) and Omsk hemorrhagic fever (OHV) as well as by mosquitoes of Japanese encephalitis (JE), North Nile (NN), Murrey Valley encephalitis (MVE), Saint-Lois encephalitis (SLE), dengue 1-4 and of yellow fever (YF). The probes are synthetic deoxyoligonucleotides with the 18-20 long basis and complementary for the RNA fragments defined by computer analysis. The thus obtained probes, which specifically hybridize themselves with the sets of the TBE virus or of the OHV virus and do not hybridize themselves with other TBE viruses' sets. Group-specific probes for YE and dengue viruses as well as virus-specific probes, which are able to detect each of the above viruses without any cross effects, were suggested for indexing and identifying the flaviviruses transmitted by mosquitoes.

References

  1. Злобин В. И., Кветкова Э. А., Наволокин О. В. и др. Сравнение трех экспресс-методов индикации вируса клещевого энцефалита // Вопр. вирусол. - 1990. - № 1. - С. 57-59.
  2. Злобин В. И., Демина Т. В., Беликов С. И. и др. Анализ генетической вариабельности штаммов вируса клещевого энцефалита по первичной структуре фрагмента гена белка оболочки Е // Там же. - 2001. - № 1. - С. 13-16.
  3. Калишер Ч. X. (Calisher С. Н.) Антигенная классификация и таксономия флавивирусов (семейство Flaviviridae). Создание универсальной системы для таксономии вирусов, вызывающих клещевой энцефалит // Acta Virol. - 1988. - Т. 32. - С. 464-473.
  4. Плетнев А. Г., Ямщиков В. Ф., Блинов В. М. Нуклеотидная последовательность генома и полная аминокислотная последовательность полипротеина вируса клещевого энцефалита // Биоорган, химия. - 1989. - Т. 15, № 11. - С. 1504-1521.
  5. Шаманин В. А., Злобин В. И., Плетнев А. Г. Фрагмент нуклеиновой кислоты - зонд для выявления и идентификации флавивирусов. - Пат. № 1678835. -Роспатент. - 1993.
  6. Шаманин В. А., Злобин В. И., Плетнев А. Г. Фрагмент нуклеиновой кислоты - зонд для выявления и идентификации флавивирусов, переносимых комарами. Пат. № 1678837. - Роспатент. - 1993.
  7. Brinton M. A., Dispoto J. H. Sequence and secondary structure analysis of 5-terminal region of flavivirus genome RNA // Virology. - 1988. - Vol. 153. - P. 290-299.
  8. Felsenstein J. PHYLIP (Philogeny Interference Package) version 3.5 с Distributed by author. - Washington, 1993.
  9. Gritsun T. S., Frolova T. V., Pogodina V. V. et al. Nucleotide and deduced amino-acid sequence of the envelope glycoprotein of Omsk hemorragic fever virus comparison with other flaviviruses // J. Gen. Virol. - 1993. - Vol. 74. - P. 287-291.
  10. Hahn C. S., Galler R., Hunkapiller T. et al. Nucleotide sequence of Dengue 2 RNA and comparison of the encoded proteins with those of other flavivirus // Virology. - 1988. - Vol. 162. - P. 167-180.
  11. Lobigs M., Marshall I. D., Weir R. C., Dalgamo L. Murrey Valley encephalitis virus field strains Australia and Papua New Guinea studies on the sequence of the major envelope protein gene and virulence for mice // Ibid. - Vol. 165. - P. 245-255.
  12. Mandl С. W., Heinz F. X., Kunz Ch. Sequence of the structural proteins of tick-borne encephalitis virus (western subtype) and comparative analysis with other flaviviruses // Ibid. - Vol. 166. - P. 197-205.
  13. Mandl С. W., Iacono-Connoris L., Waller G. et al. Sequence of the genes encoding the structural proteins of the low-virulence tick-borne flaviviruses Langat TP21 and Yelantsev // Ibid. - 1991. - Vol. 185. - P. 891-895.
  14. Mandl С. W., Holzmann H., Kunz C., Heinz F. Complete genomic sequence of Powassan virus evaluation of genetic elements in tick-borne versus mosquito-borne flaviviruses // Ibid. - 1993. - Vol. 194. - P. 173-184.
  15. Rice С. M., Lenches E. M., Eddy S. R. et al. Nucleotide sequence of yellow fever virus: Implication for flavivirus gene expression and evolution // Science. - 1985. - Vol. 229, N 4715. - P. 726-733.
  16. Shamanin V. A., Pletnev A. G., Rubin S. G., Zlobin V. I. Differentiation of strfains of tick-borne encephalitis virus by means of RNA-DNA hybridization // J. Gen. Virol. - 1990. - Vol. 71. - P. 1505-1515.
  17. Shiu S. Y. W., Aires M. D., Gould F. A. Genomic sequence of the structural proteins of Louping ill virus: comparative analysis with tick-borne encephalitis virus // Virology. - 1991. - Vol. 180.- P. 411-415.
  18. Sumiyoshi H., Mori C., Fuke I. et al. Complete nucleotide sequence of the Japanese encephalitis virus genome // Ibid. - 1987. - Vol. 161. - P. 497-510.
  19. Venugopal K., Buckley A., Reid H. W., Gould F. A. Nucleotide sequence of the envelope glycoprotein of Negishi virus shows very close homology to Louping ill virus // Ibid. - 1992. - Vol. 190. - P. 515-521.
  20. Venugopal K., Gritsun Т., Lashkevich V. A., Could F. A. Analysis of the structural proteins gene seqeunce shows Kyasanur-forest disease virus as a distinct member of the tick-borne encephalitis virus complex // J. Gen. Virol. - 1994. - Vol. 75. - P. 227-232.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2003 Zlobin V.I., Pogodina V.V., Drokin D.A., Gazo M.K., Bochkova N.G., Djioyev Y.P., Demina T.V., Belikov S.I., Kozlova I.V., Verkhozina M.M.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies