Specific identification of Orthopoxviruses in oligonucleotides micro-chips


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Abstract

A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).

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Copyright (c) 2003 Mikheev M.V., Lapa S.A., Shchelkunov S.N., Chikova A.K., Mikhailovich V.M., Sobolev A.Y., Babkin I.V., Gryadunov D.A., Bulavkina M.A., Guskov A.A., Sokunova E.В., Kochneva G.V., Blinov V.M., Sandakhchiev L.S., Zasedatelev A.S., Mirzabekov A.D.

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