Problems of Virology

The article presents historical aspects and key achievements of the Department of Virus Ecology (DVE) with the Scientific and Practical Center for Influenza Ecology and Epidemiology, which was established in 1969 at the D.I. Ivanovsky Institute of Virology of Academy of Medical Sciences of the USSR. For over 50 years, the DVE has been devoted to addressing fundamental issues in virus ecology, including the formation of viral populations in nature, and conducting comprehensive large-scale studies in the interest of the state’s biosecurity. The department’s primary focus is on particularly dangerous (arboviral) and socially significant (influenza and other acute respiratory viral infections, parenteral hepatitis) viral infections. As a result of this extensive work in the Northern Eurasia region, over 2,000 strains of zoonotic viruses (17 genera, 8 families), ecologically linked to various arthropod vectors and vertebrate hosts, have been isolated. Many of them have been registered in international catalogs as new species. The role of these isolated viruses in human pathology has been studied, new viral infections have been described, and diagnostic preparations have been developed. The scientific results obtained by the department are of high priority and internationally recognized.

Human herpes virus 6A and human herpes virus 6B (HHV-6A and HHV-6B) are ubiquitous viruses. The spectrum of clinical manifestations of HHV-6A/B infections is quite wide. The current understanding of the natural history and laboratory diagnosis of HHV-6A and HHV-6B, including their chromosome-integrated form, serves the basis for development of the tools for HHV-6 epidemiological monitoring. This article addresses the epidemiology and diagnosis of infections caused by these viruses, including ones in patients after transplantation of solid organs and allogeneic hematopoietic stem cells.

The purpose of this work was to determine the characteristics of the circulation of various viral respiratory pathogens during the epidemic season 2022–2023 against the background of the ongoing evolutionary variability of SARS-CoV-2.

Materials and methods. The article uses methods used in «traditional» and «hospital» epidemiological surveillance of acute respiratory viral infections.

Results and discussion. The period from October 2022 to September 2023 was characterized by early and high activity of influenza A(H1N1)pdm09 virus, which was replaced by influenza B virus. The antigenic and genetic properties of strains were closely related to influenza vaccines viruses recommended by WHO experts for the current season. The effectiveness of influenza vaccines was confirmed (75.0%). All of the studied influenza A(H1N1)pdm09, A(H3N2) and B epidemic strais retained sensitivity to drugs with antineuraminidase activity. The structure and share of other ARVI pathogens have changed somewhat compared to the previous season: There was a tendency to increase the activity of HAdV and HMPV; almost equivalent activity of HRsV, HRV, HCoV and HBoV; and a decrease in HPIV activity. At the same time, the frequency of other ARVI pathogens did not reach the indicators of the pre-pandemic COVID-19 period. The rationale for updating the composition of influenza vaccines for the countries of the Northern Hemisphere in the 2023–2024 season is given.

Introduction. Hepatitis B virus (HBV) remains a pressing global public health concern. The clinical course of the disease, particularly its tendency towards chronicity and response to therapy, is significantly influenced by the HBV genotype and specific mutations. There is an imperative need for a straightforward, highly sensitive, and dependable method for whole genome sequencing of HBV.

Objective. Development and testing of an amplification panel for HBV whole-genome sequencing.

Materials and methods. We introduce an NGS amplification panel designed for genome sequencing of HBV on the Illumina platform. A panel consisting of 54 primers, divided into 2 pools and amplifying overlapping regions of the HBV genome up to 300 bp in length, was tested on 246 HBV DNA samples.

Results. The studied samples represented a genotypic diversity of the virus, with a pronounced predominance of the genotype specific to the Moscow region: 216, 27, 2, and 1 sample were identified as genotype D, A, B, and E, respectively. Five samples contained at least one mutation associated with antiviral therapy resistance, and twenty-three samples contained at least one mutation associated with vaccine escape described in the literature.

Conclusion. The present paper describes the stages of whole-genome sequencing of HBV, provides a laboratory protocol, nucleotide sequences of the primers and an approach to the data analysis. Using a list of clinical samples as example, the reliability of the panel is shown. The HBV panel holds immense potential for utilization in scientific research, epidemiological monitoring, and advancement of personalized medicine approaches.

In the first 20 years of the 21^st century, almost annual outbreaks of viral infections were recorded. It is becoming increasingly clear that until humanity changes its destructive attitude towards nature, epidemics of deadly diseases will continue to occur. A question arises for discussion: is the existing arsenal of antiviral agents sufficient to withstand the current unfavorable socio-economic situation in the world?