Antiviral activity of the dihydroquercetin during the Coxsackievirus В4 replication in vitro

Cover Page


Cite item

Full Text

Abstract

A study of the antiviral activity of antioxidants against viral infections is believed to be essential for creating complex antiviral agents. Dihydroquercetin is considered as the most active antioxidant extracted from larix gmelinii. In this work, we present results of experiments of the antiviral properties of dihydroquercetin against a member of the family picornaviridae – Coxsackievirus B4 in vitro. We have estimated that dihydroquercetin reduces viral titers at 100 µg/ml concentration as compared with control of virus. We have shown using the plaque assay that cpe of virus is reduced in the presence of dihydroquercetin at 100 µg/ml. Study of the phase of viral life cycle, in which dihydroquercetin acted, demonstrated that the highest efficacy of the antiviral therapy was reached at early stages of virus reproduction (1-3 hours post infection). These results show that dihydroquercetin has antiviral property against Coxsackievirus B4. This drug and other antioxidants can be tested as inhibitors of viral replication.

About the authors

A. V. Galochkina

Research Institute of Influenza

Author for correspondence.
Email: nastyagalochkina@yandex.ru

Anastasiya Galochkina, research assistant

197376, St. Petersburg

Россия

V. V. Zarubaev

Research Institute of Influenza

Email: fake@neicon.ru
197376, St. Petersburg Россия

O. I. Kiselev

Research Institute of Influenza

Email: fake@neicon.ru
197376, St. Petersburg Россия

V. A. Babkin

A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences

Email: fake@neicon.ru
664033, Irkutsk Россия

L. A. Ostroukhova

A.E. Favorsky Irkutsk Institute of Chemistry, Siberian Branch, Russian Academy of Sciences

Email: fake@neicon.ru
664033, Irkutsk Россия

References

  1. Энтеровирусная инфекция, лечение, причины, симптомы, профилактика. Available at: http://www.pitermed.com/simptomybolezni/?cat=6&word=55036 (in Russian)
  2. Suvi Rasilainen. Coxsackie Virus Infections and Oxidative Stress as Mediators of Beta Cell Damage: Diss. Helsinki; 2004.
  3. Zaoutis T., Klein J.D. Enterovirus Infections. Pediatr. Rev. 1998; 19(6): 183–91.
  4. Varela-Calvino R., Peakman M. Enteroviruses and type 1 diabetes. Diabetes Metab. Res. Rev. 2003; 19(6): 431–41.
  5. Kuo R.L., Shih S.R. Strategies to develop antivirals against enterovirus 71. Virol. J. 2013; 10: 28.
  6. Piconi L., Quagliaro L., Ceriello A. Oxidative stress in diabetes. Clin. Chem. Lab. Med. 2003, 41(9): 1144–9.
  7. Kuo R.L., Shih S.R. Strategies to develop antivirals against enterovirus 71. Virol. J. 2013; 10: 28.
  8. Feng Q., Langereis M.A., Lork M., Nguyen M., Hato S.V., Lanke K. et al. Enterovirus 2Apro targets MDA5 and MAVS in infected cells. J. Virol. 2014; 88(6): 3369–78.
  9. Pietta P.G. Flavonoids as antioxidants. J. Nat. Prod. 2000; 63(7): 1035–42.
  10. Gibellini L., Pinti M., Nasi M., Montagna J.P., De Biasi S., Roat E. et al. Quercetin and cancer chemoprevention. Evid. Based Complement. Alternat. Med. 2011; 2011: 591356.
  11. U-критерий Манна–Уитни. Available at: http://medstatistic.ru/theory/mann.html (in Russian)
  12. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 1983; 65(1–2): 55–63.
  13. West I.C. Radicals and oxidative stress in diabetes. Diabet.Med. 2000; 17(3): 171–80.
  14. Piconi L., Quagliaro L., Ceriello A. Oxidative stress in diabetes. Clin. Chem. Lab. Med. 2003, 41(9): 1144–9.
  15. Han S.N., Meydani S.N. Antioxidants, cytokines and influenza infection in aged mice and elderly humans. J. Infect. Dis. 2000; 182(Suppl. 1): S74–80.
  16. Nathan C., Cunningham-Bussel A. Beyond oxidative stress: an immunologist`s guide to reactive oxygen. Nat. Rev. Immunol. 2013; 13(5): 349–61.
  17. Ho H.Y., Cheng M.L., Weng S.F., Leu Y.L., Chiu D.T. Antiviral effect of epigallocatechin gallate on enterovirus 71. J. Agric. Food Chem. 2009; 57(14): 6140–7.
  18. Robin V., Irurzun A., Amoros M., Boustie J., Carrasco L. Antipoliovirus flavonoids from Psiadia dentate. Antivir. Chem. Chemother. 2001; 12(5): 283–91.
  19. De Palma A.M., Vliegen I., De Clercq E., Neyts J. Selective inhibitors of picornavirus replication. Med. Res. Rev. 2008; 28(6): 823-84. Available at: www.interscience.wiley.com

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Galochkina A.V., Zarubaev V.V., Kiselev O.I., Babkin V.A., Ostroukhova L.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.