Email this article LOW CONCENTRATIONS OF HEPATITIS C VIRUS RNA IN SEROLOGICALLY MILD INFECTION
- Authors: Yaroslavtseva N.G.1, Tikhomirov D.S.1, Nikolaeva L.I.2, Dedova A.V.2, Ovchinnikova E.N.1, Misko O.N.1, Romanova T.Y.1, Makhnovskiy P.I.2, Grishechkin A.E.2, Tupoleva T.A.1
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Affiliations:
- National Medical Research Center of Hematology
- D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
- Issue: Vol 64, No 1 (2019)
- Pages: 30-35
- Section: ORIGINAL RESEARCH
- Submitted: 20.01.2020
- Published: 20.02.2019
- URL: https://virusjour.crie.ru/jour/article/view/231
- DOI: https://doi.org/10.18821/0507-4088-2019-64-1-30-35
- ID: 231
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Abstract
Occult HCV infection (OCI) provides significant interest recently. HCV RNA in this case can be detected not in plasma, but in blood cells and/or in liver tissue. In case of antibody genesis impairment anti-HCV detection may lead to negative or “uncertain” result. The aim of the study was to estimate infection type in blood donors and patients with hematological diseases by exploration of samples with uncertain anti-HCV detection results. Blood samples of 30 180 potential blood donors’ and 4322 patients with hematological diseases were tested. Comparative analysis of wide pattern of HCV markers was performed. 33 blood donors and 42 patients were enrolled in follow-up examination. Uncertain results of Anti-HCV detection in donors’ samples were in 0.18% of cases. Follow-up examination of 33 donors provided discordant results using immunochemiluminescence assay and ELISA. 15.2% donors’ samples contained HCV RNA in low concentration. Follow-up observation of 42 patients with incomplete antiviral antibody pattern showed HCV RNA presence in 40.5% cases (21.4% high viremia and 19.0% low viremia). Samples with low RNA concentration contained low titers of anti-core antibodies. Samples with high titers of anti-core antibodies contained high HCV RNA level. Uncertain results of anti-HCV in 15.2% of potential blood donors’ samples were confirmed by detection of HCV RNA in low concentration. It proved OCI presence in these individuals and called for testing for wide pattern of HCV markers in addition to routine screening. Patients with hematological diseases showed low level of HCV RNA along with low titers of antibodies against one or two viral antigens.
About the authors
N. G. Yaroslavtseva
National Medical Research Center of Hematology
Author for correspondence.
Email: ngyar@yandex.ru
Russian Federation
D. S. Tikhomirov
National Medical Research Center of Hematology
Email: noemail@neicon.ru
Russian Federation
L. I. Nikolaeva
D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Russian Federation
A. V. Dedova
D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Russian Federation
E. N. Ovchinnikova
National Medical Research Center of Hematology
Email: noemail@neicon.ru
Russian Federation
O. N. Misko
National Medical Research Center of Hematology
Email: noemail@neicon.ru
Russian Federation
T. Yu. Romanova
National Medical Research Center of Hematology
Email: noemail@neicon.ru
Russian Federation
P. I. Makhnovskiy
D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Russian Federation
A. E. Grishechkin
D.I. Ivanovsky Institute of Virology «National Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Russian Federation
T. A. Tupoleva
National Medical Research Center of Hematology
Email: noemail@neicon.ru
Russian Federation
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).