Email this article DIAGNOSTIC CAPACITY OF DETECTION OF SPECIFIC ANTIBODIES TO PANDEMIC INFLUENZA A(H1N1)PDM09 VIRUS
- Authors: Mukasheva E.A.1, Nikolaeva L.I.1, Makhnovsky P.I.1, Kirillova E.S.1, Kolobukhina L.V.1, Merkulova L.N.1, Kruzhkova I.S.1, Malyshev N.A.1, Burtseva E.I.1
-
Affiliations:
- D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
- Issue: Vol 62, No 3 (2017)
- Pages: 109-114
- Section: ORIGINAL RESEARCH
- Submitted: 20.01.2020
- Published: 20.06.2017
- URL: https://virusjour.crie.ru/jour/article/view/151
- DOI: https://doi.org/10.18821/0507-4088-2017-62-3-109-114
- ID: 151
Cite item
Full Text
Abstract
Serologic studies occupy a significant place in influenza diagnosis. The article presents an analysis of the developed experimental version of ELISA test-systems for the detection of specific antibodies to the virus influenza A(H1N1)pdm09, and their dynamics at different stages of infection as compared with those of the traditional HAI method. The study included 20 paired samples of serum from patients hospitalized at different stages of the disease with etiology associated with the influenza virus A(H1N1)pdm09. Two groups were formed on the basis of HAI data, which showed the presence or absence of significant growth of specific antibodies to the influenza virus A(H1N1)pdm09. The control group consisted of 20 serum samples from individuals without influenza but with chronic hepatitis C. To examine the virus specific antibody two types of ELISA test systems were used. The first system was intended for the detection of IgM to the influenza virus A(H1N1)pdm09; the second was used for revealing specific IgG. The study showed the accuracy and specificity of detectable IgM and IgG to the virus influenza A(H1N1)pdm09. The dynamics of specific IgG titers in 15 of the 20 pairs of sera was reliable. The increase in titers was more pronounced than in the HAI. IgM against influenza virus could be detected up to 10 days, although reliable dynamics of these antibodies was not detected in paired samples. The test system was specific for the determination of both IgG and IgM antibodies to the influenza virus A(H1N1)pdm09 and significantly more sensitive than HAI. Using this ELISA test system, it is possible to monitor the dynamics of IgG to this virus even in the absence of diagnostic increases in antibody titers in HAI.
Keywords
About the authors
E. A. Mukasheva
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Author for correspondence.
Email: mukasheva_evgeniya@mail.ru
Россия
L. I. Nikolaeva
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
P. I. Makhnovsky
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
E. S. Kirillova
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
L. V. Kolobukhina
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
L. N. Merkulova
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
I. S. Kruzhkova
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
N. A. Malyshev
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
E. I. Burtseva
D.I. Ivanovsky Institute of Virology, Federal State Budgetary Institution «Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»
Email: noemail@neicon.ru
Россия
References
- Sominina A., Burtseva E., Eropkin M., Karpova L., Zarubaev V., Smorodintseva E. et al. Influenza surveillance in Russia based on epidemiological and laboratory data for the period from 2005 to 2012. Am. J. Infect. Dis. 2013; 9(3): 77-93.
- Molinari N.A., Ortega-Sanchez I.R., Messonnier M.I., Thompson W.W., Wortley P.M., Wientraub E. et al. The annual impact of season influenza in the US: measuring disease burden and costs. Vaccine. 2007; 25(27): 5086-96.
- World Health Organization. Flu News Europe. Available at: http://flunewseurope.org
- Harmon M.W., Rota P.A., Walls H.H., Kendal A.P. Antibody response in humans to influenza virus type B host cell-derived variants after vaccination with standard (egg-derived) vaccine or natural infection. J. Clin. Microbiol. 1988; 26(2): 333-7.
- Львов Д.К., ред. Руководство по вирусологии. Вирусы и вирусные инфекции человека и животных. М.: Медицинское информационное агентство; 2013.
- Кузнецов О.К., Степанова Л.А. Продолжительность защиты от гриппа после инфицирования и вакцинации. Эпидемиология и вакцинопрофилактика. 2009; (4): 29-38.
- Rowe T., Abernathy R.A., Hu-Primmer J., Thompson W.W., Lu X., Lim W. et al. Detection of antibody to avian influenza A(H5N1) virus in human serum by using a combination of serologic assays. J. Clin. Microbiol. 1999; 37(4): 937-43
- Войцеховская Е.М., Вакин В.С., Васильева А.А., Кузнецова Е.В., Кривицкая В.З., Соминина А.А. Применение метода радиального гемолиза для выявления антител к вирусам гриппа птиц A(H5N1) и пандемическому вирусу A(H1N1)pdm09. Клиническая лабораторная диагностика. 2012; (12): 28-31
- Cui S., Tong G.A. A chromatographic strip test for rapid detection of one lineage of the H5 subtype of highly pathogenic avian influenza. J. Vet. Diagn. Invest. 2008; 20(5): 567-71.
- Prabakaran M., Ho H.T., Prabhu N., Velumani S., Szyporta M., He F. et al. Development of epitope-blocking ELISA for universal detection of antibodies to human H5N1 influenza viruses. PloS One. 2009; 4(2): e4566.
- He F., Prabakaran M., Tan Y., Indira K., Kumar S.R., Kwang J. Development of dual-function ELISA for effective antigen and antibody detection against H7 avian influenza virus. BMC Microbiol. 2013; 13: 219-27.
- van Baalen C.A., Els L., Sprong R., van der Vries E., van Beek R., Osterhaus A.D. et al. Detection of nonhemagglutinating influenza A(H3) viruses by enzyme-linked immunosorbent assay in quantitative influenza virus culture. J. Clin. Microbiol. 2014; 52(5): 1672-7.
- Davies H.W., Appleyard G., Cunnighan P., Pereira M.S. The use of continuous cell line for the isolation of influenza virus. Bull. World Health Organ. 1978; 56(6): 991-3.
- Markwell M.A., Haas S.M., Bieber L.L., Tolbert N.E. Modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 1978; 87(1): 206-11.
- Julkunen I., Pyhala R., Hovi T. Enzyme immunoassay, complement fixation and hemagglutination inhibition tests in the diagnosis of influenza A and B virus infections. Purified hemagglutinin in subtype-specific diagnosis. J. Virol. Methods. 1985; 10(1): 75-84.
- Роганова И.В., Суздальцев А.А. Грипп: монография. Самара: Ас Гард; 2013
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).