Preclinical studies of live intranasal H5N1 influenza vaccine with the deleted HS1 gene
- Issue: Vol 56, No 6 (2011)
- Pages: 19-22
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.12.2011
- URL: https://virusjour.crie.ru/jour/article/view/12109
- ID: 12109
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Abstract
The paper gives the results of evaluating the efficiency of delNS1 pandemic H5N1 vaccine candidate VN1203delNS1 which was constructed by reverse genetics on the basis of influenza virus strain A/Vietnam/1203/04. The safety, immunogenicity and cross-protection of the vaccine strain against different H5N1 virus clades were demonstrated in mouse and macaque models. The results showed the possibility of designing a new-generation replication-deficient intranasal influenza vaccine, by applying an approach to deleting the NS1 pathogenicity factor, an antagonist of the interferon system.
References
- Egorov A., Brandt S., Sereinig S. et al. Transfectant influenza A viruses with long deletions in the NS1 protein grow efficiently in Vero cells // J. Virol. - 1998. - Vol. 72. - P. 6437-6441.
- Ferko В., Stasakova J., Romanova J. et al. Immunogenicity and protection efficacy of replication-deficient influenza A viruses with altered NS1 genes // J. Virol <http://Virol.-2004.-Vol.78.-P>. - 2004. <http://Virol.-2004.-Vol.78.-P> - Vol. 78 <http://Virol.-2004.-Vol.78.-P>. - P. 13 037-13 045.
- Garcia-Sastre A., Egorov A., Matassov D. et al. Influenza A virus lacking the NS1 gene replicates in interferon-deficient systems // Virology. - 1998. - Vol. 252. - P. 324-330.
- Hoffmann E., Neumann G., Kawaoka Y. et al. A DNA transfection system for generation of influenza A virus from eight plasmids // Proc. Natl. Acad. Sci. USA. - 2000. - Vol. 97. - P. 6108-6113.
- Horimoto T., Takada A. et al. The development and characterization of H5 influenza virus vaccines derived from a 2003 human isolate // Vaccine. - 2006. - Vol.24. - P. 3669-3676.
- Romanova J., Krenn B. M., Wolschek М. et al. Preclinical evaluation of a replication-deficient intranasal DeltaNSl H5N1 influenza vaccine // PLoS One. - 2009. - Vol. 4, N 6. - P. e5984.
- Stasakova J., Ferko В., Kittel C. et al. Influenza A mutant viruses with altered NS1 protein function provoke caspase-1 activation in primary human macrophages, resulting in fast apoptosis and release of high levels of interleukins l beta and 18 // J. Gen. Virol. - 2005. - Vol. 86. - P. 185-195.
- Wacheck V., Egorov A., Groiss F. et al. A novel type of influenza vaccine: safety and immunogenicity of replication-deficient influenza virus created by deletion of the interferon antagonist NS1 // J. Infect. Dis. - 2010. - Vol. 201, N 3. - P. 354-362.
- Wressnigg N., Voss D., Wolff T. et al. Development of live-attenuated influenza В DeltaNSl intranasal vaccine candidate // Vaccine. - 2009. - Vol. 27. - P. 2851-2857.