Cytotoxic properties of diagnostic rabbit sera to enteroviruses Specific features and localization


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Abstract

Hyperimmune rabbit enterovirus-neutralizing diagnostic sera have a pronounced cytotoxic effect on the test tissue culture RD, HEp-2, and Vero cells and primary renal cells from African green monkeys (Cercopithecus aethiops). The specific features of this developing toxic effect, such as reduced proliferative activity and partial cytolysis, were revealed. The time course of changes in the generation of serum cytotoxicity during an immune response to repeated antigenic stimulation of donor animals is described. There is experimental evidence for no association of serum toxicity with specific neutralizing antibodies and with antibodies to cell culture antigens. Affinity chromatography is one of the possible techniques for purification of cytotoxic sera.

References

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  35. Colley D. G., Waksman B. H. Cytotoxic effect of normal rabbit serum on rat lymphoid cells // Transplantation. - 1970. - Vol. 9. - P. 395.
  36. Committee on the Enteroviruses, National Foundation for Infantile Paralysis. The Enteroviruses // Am. J. Publ. Hlth. - 1957. - Vol. 47. - P. 1556-1566.
  37. Chang R., Pennell R., Keller W. et al. Macromolecular growth requirements of human cells in continuous culture // Proc. Soc. Exp. Biol. - 1959. - Vol. 102, N 1. - P. 213-217.
  38. Fedoroff S., Cook B. Interaction between cells and cytotoxic sera in cell cultures // Pathol. Biol. - 1961. - Vol. 9, N 5-6. - P. 584-590.
  39. Fedoroff S., Webb S. J. Natural cytotoxic antibodies in human blood sera with reach with mammalian cell and bacteria // Nature. - 1962. - Vol. 193. - P. 80-81.
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  41. Herberman R. B. Cytolysis of human cell by antibodies in normal rabbit serum // Transplantation. - 1969. - Vol. 8. - P. 813.
  42. Hilden J., Ronnike F. The toxic effects of human serum on Erlich ascites tumor cells in vitro culture // Res. Exp. Med. - 1974. - Vol. 163. - P. 185-197.
  43. Kierszenbaum F., Budzko D. B. Cytotoxic effects of normal sera on lymphoid cells. I. Antibody-independent killing of heterologous thymocytes by guinea pig, rabbit and human sera: role of the alternative pathway of complement activation // Cell Immunol. - 1977. - Vol. 29, N 1. - P. 137-146.
  44. National Foundation for Infantile Paralysis. Preparation and use of ECHO virus antisera for types 1 to 14 and Coxsackie virus antisera for type B 1 to B 5 and A 9, prepared by Microbiological Association Inc. 1957.
  45. Ollert M. W., David K., Schmitt C. et al. Normal human serum contains a natural IgM antibody cytotoxic for human neuroblastoma cells // Proc. Natl. Acad. Sci. USA. - 1996. - Vol. 93. - P. 4498-4503.
  46. Palacios G., Oberste M. S. Enteroviruses as agents of emerging infectious diseases // J. Neurovirol. - 2005. - Vol. 11. - P. 424-433.
  47. Pallansch M. A., Roos R. P. Enteroviruses: poliovirus, coxsackieviruses, echoviruses, and never entroviruses // Fields Virology / Eds D. M. Knipe et al. - Philadelphia, 2001. - P. 723-775.
  48. Reif A. E. Immune cytolysis of mouse thymic lymphocytes // J. Immunol. - 1963. - Vol. 91. - P. 557-567.
  49. Sandberg G., Rydlander L., Stenvinkel C. Purification, identification and elimination of a natural human serum antibody with cytotoxic effect on guinea pig thimocytes // Int. Arch. Allergy Appl. Immunol. - 1987. - Vol. 83, N 1. - P. 57-63.
  50. Seung Yong Park, Kojima M., Suzuki G., Shinohara N. Effective blocking of natural cytotoxoty of young rabbit serum on murine thimocytes by high concentration of glucose in complement-dependent cytotoxicity method // J. Immunol. Meth. - 1992. - Vol. 154. - P. 109-119.
  51. Sternova H., Cech K., Nouza K. et al. Cytotoxicity of normal rabbit serum for chicken lymphoid cells. I. Localization of the activity // Immunology. - 1976. - Vol. 30. - P. 925-927.
  52. Van Wezel A. L., van Herwaarden J. A., van de Heuvel-de Rijk E. W. Large-scale concentration and purification of virus suspension from microcarrier culture for the preparation of inactivated virus vaccines // Dev. Biol. Stand. - 1979. - Vol. 42. - P. 65-69.

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