ISOLATION AND PHYLOGENETIC ANALYSIS OF FELINE CALICIVIRUS IN SIBERIA

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Abstract

The results of the study of the distribution of calicivirus infection in a population of domestic cats of different breeds, contained individually or the group method, the virus isolation in the cell culture and a comparative phylogenetic analysis of their nucleotide sequences with published sequences of reference field and vaccine strains of Feline calicivirus (FCV) from other countries: USA, Germany, Japan, China and Korea are presented. Clinical signs of infection were found in 14.3% of the animals examined. After several passages in the primary kidney cells of the kitten embryo, seven cytopathogenic isolates FCV were isolated: 1 - from a cat with an acute infection, 5 - subclinical infection, 1 - systemic infection. They were adapted to continuous FK-81 cells in which they reached a maximum infectious activity of 10.0 ± 1.15 lg TCD 50 / cm3. Based on the sequence analysis of the open reading frame 2 region of the viral genome Eshli strain showed a close relationship with strain KM016908 from China with the identity of the nucleotide sequences between them of 81.0%. The results of the investigations showed that FCV isolates obtained from animals on the territory of Siberia are genetically different from strains included to imported vaccines used to prevent disease in Russian Federation and also among themselves. This causes a decrease in the effectiveness of preventive measures. In nurseries that do not have contacts and connections between themselves but located in the same geographic region FCV populations may have some genetic differences. A close relationship of some field isolates with strains from other countries geographically located so far from the Siberian region has been revealed. Studies on the molecular epizootology of caliciviruses are important in the development of test systems and the monitoring of the spread of strains in Russia.

About the authors

T. I. Glotova

Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East

Author for correspondence.
Email: t-glotova@mail.ru
Россия

O. V. Semenova

Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: noemail@neicon.ru
Россия

A. A. Nikonova

Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: noemail@neicon.ru
Россия

A. G. Glotov

Siberian Federal Scientific Centre of Agro-Biotechnologies of the Russian Academy of Science, Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: noemail@neicon.ru
Россия

Y. V. Vyatkin

Ltd. «AkademDzin»

Email: noemail@neicon.ru
Россия

A. A. Bondar

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences

Email: noemail@neicon.ru
Россия

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  52. Edgar R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic. Acids Res. 2004; 32(5): 1792-7.
  53. Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Syst. Biol. 2010; 59(3): 307-11.
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Copyright (c) 2018 Glotova T.I., Semenova O.V., Nikonova A.A., Glotov A.G., Vyatkin Y.V., Bondar A.A.

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