COMPARISON OF INFLUENZA A VIRUS INHIBITION IN VITRO BY SIRNA COMPLEXES WITH CHITOSAN DERIVATIVES, POLYETHYLENEIMINE AND HYBRID POLYARGININE-INORGANIC MICROCAPSULES

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Abstract

Anti-influenza drugs and vaccines have a limited effect due to the high mutation rate of virus genome. The direct impact on the conservative virus genome regions should significantly improve therapeutic effectiveness. The RNA interference mechanism (RNAi) is one of the modern approaches used to solve this problem. In this work, we have investigated the antiviral activity of small interfering RNA (siRNA) against the influenza A/PR/8/34 (H1N1), targeting conserved regions of NP and PA. Polycations were used for intracellular siRNA delivery: chitosan’s derivatives (methylglycol and quaternized chitosan), polyethyleneimine, lipofectamine, and hybrid organic/non-organic microcapsules. A comparative study of these delivery systems with fluorescent labeled siRNA was conducted. The antiviral activity of three small interfering RNAs targeting the NP (NP-717, NP-1496) and PA (PA-1630) influenza A viruses genes was demonstrated, depending on the chosen carrier. The most effective intracellular delivery and antiviral activity were observed for hybrid microcapsules.

About the authors

A. V. Petrova-Brodskaya

Research Institute of Influenza; Peter the Great St. Petersburg Polytechnic University

Author for correspondence.
Email: alexandra.b_05@mail.ru
Россия

A. B. Bondarenko

Research Institute of Influenza; St. Petersburg State University

Email: noemail@neicon.ru
Россия

A. S. Timin

Peter the Great St. Petersburg Polytechnic University; National Research Tomsk Polytechnic University

Email: noemail@neicon.ru
Россия

M. A. Plotnikova

Research Institute of Influenza

Email: noemail@neicon.ru
Россия

M. V. Afanas’Ev

Research Institute of Influenza; St. Petersburg State University

Email: noemail@neicon.ru
Россия

A. A. Semenova

St. Petersburg State Chemical Pharmaceutical Academy

Email: noemail@neicon.ru
Россия

K. I. Lebedev

Research Institute of Influenza

Email: noemail@neicon.ru
Россия

A. N. Gorshkov

Research Institute of Influenza; Institute of Cytology

Email: noemail@neicon.ru
Россия

M. Yu. Gorshkova

A.V. Topchiev Institute of Petrochemical Synthesis

Email: noemail@neicon.ru
Россия

V. V. Egorov

Research Institute of Influenza

Email: noemail@neicon.ru
Россия

S. A. Klotchenko

Research Institute of Influenza

Email: noemail@neicon.ru
Россия

A. V. Vasin

Research Institute of Influenza; Peter the Great St. Petersburg Polytechnic University

Email: noemail@neicon.ru
Россия

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Copyright (c) 2017 Petrova-Brodskaya A.V., Bondarenko A.B., Timin A.S., Plotnikova M.A., Afanas’Ev M.V., Semenova A.A., Lebedev K.I., Gorshkov A.N., Gorshkova M.Y., Egorov V.V., Klotchenko S.A., Vasin A.V.

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