Detection of bovine respiratory syncytial virus by RT-PCR


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Abstract

The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals.

References

  1. Глотов А. Г., Глотова Т. И., Котенева С. В. и др. Эпизоотическая ситуация по респираторно-синцитиальной инфекции крупного рогатого скота в хозяйствах по производству молока // Ветеринария. - 2010. - № 7. - С. 21-25.
  2. Гуненков В. В., Халенев Г. А., Сюрин В. Н. Респираторно-синцитиальная вирусная инфекция // Животноводство и ветеринария. - М., 1975. - Т. 8. - С. 70-76.
  3. Andrews A. H. Respiratory disease. - Oxford, 2004. - P. 286- 293.
  4. Baker J. C. Bovine respiratory syncitial virus // Vet. Clin. N. Am. Food Animal Pract. - 1997. - Vol. 13. - P. 425-454.
  5. Ellis J. A., Plnlihen H., West K. et al. Fatal pneumonia in adult dairy cattle associated with active infection with bovine respiratory syncytial virus // Can. Vet. J. - 1996. - Vol. 3. - P. 103-105.
  6. Hagglund S. Epidemiology, detection and prevention of respiratory virus infections in Swedish cattle with special reference to bovine respiratory syncytial virus. - Doctoral thesis Swedish University of Agricultural Sciences. - Uppsala, 2005.
  7. Larsen L. E., Tjörnehöj K., Viuff B. et al. Diagnosis of enzootic pneumonia in Danish cattle: reverse transcription-polymerase chain reaction assay for detection of bovine respiratory syncytial virus in naturally and experimentally infected cattle // J. Vet. Diagn. Invest. - 1999. - Vol. 1. - P. 416-422.
  8. Larsen L. E. Bovine respiratory syncytial virus (BRSV): A review // Acta Vet. Scand. - 2000. - Vol. 41. - P. 1-24.
  9. Valarcher J.-F., Bourhy H., Gelfi J., Schelcher F. Evaluation of a nested reverse transcription-PCR assay based on the nucleoprotein gene for diagnosis of spontaneous and experimental bovine respiratory syncytial virus infections // J. Clin. Microbiol. - 1999. - Vol. 37. - P. 1858-1862.
  10. Valarcher J.-F., Taylor G. Bovine respiratory syncytial virus infection // Vet. Res. - 2007. - Vol. 38. - P. 153-180.
  11. Valentovä V., Kovalik K., Pikal I. Detection of bovine respiratory syncytial virus in cell cultures by nested RT-PCR and use of the method for virus identification in clinical samples // Acta Vet. Brno. - 2003. - Vol. 72. - P. 115-122.
  12. Vilcek S., Elvander M., Ballagi-Pordany A., Belak S. Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples // J. Clin. Microbiol. - 1994. - Vol. 32. - P. 2225-2231.

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Copyright (c) 2011 Glotov A.G., Glotova T.I., Stroganova I.Y., Glotov A.G., Glotova T.I., Stroganova I.Y.

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