Detection of bovine respiratory syncytial virus by RT-PCR
- Issue: Vol 56, No 5 (2011)
- Pages: 34-37
- Section: Articles
- Submitted: 09.06.2023
- Published: 15.10.2011
- URL: https://virusjour.crie.ru/jour/article/view/12108
- ID: 12108
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Abstract
The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals.
References
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