The generation and characteristics of reassortant influenza A virus with H5 hemagglutinin and other genes from the apathogenic virus H6N2


Cite item

Full Text

Abstract

The experimental reassortant vaccine strain VN-gull (H5N2) containing H5 hemagglutinin (HA) with a removed polybasic site in the connecting peptide and other genes from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (gull/M) was obtained using a two-step protocol. At Step 1, the reassortant with HA of A/Vietnam/1203/04-PR8/CDC-RG and other genes from cold-adapted A/Leningrad/17/47 (VN-Len) viruses was generated due to selection with antibody to H2N2 at 26ºC. At Step 2, the reassortant VN-gull was obtained by replacing all genes from Len with those from gull/M due to selection with antibody to H6N2 at 39ºC.
The reassortant VN-Len was apathogenic and the reassortant VN-gull was weakly virulent in mice. Both gave rise to specific antibodies and 4 weeks after single inoculation they provided complete protection against further challenge with highly pathogenic H5N1 virus A/chicken/Kurgan/3/05 (H5N1) (Ku-Len).
The chickens infected with live VN-gull virus showed nether clinical symptoms, nor fecal virus excretion; nevertheless, they gave rise to antibodies and were protected from the further challenge with A/chicken/Kurgan/3/2005.
The high yield, safety, and protectivity of VN-Len and Ku-Len made them promising strains for the production of inactivated and live vaccines against H5N1 viruses.

References

  1. Ломакина Н.Ф., Кропоткина Е. А., Гамбарян А. С, Ямникова С. С. Использование ПЦР с дифференцирующими праймерами для определения генного состава реассортантов вируса гриппа А // Ветеринарна медицина (Украина, Харьков). - 2007. - Т.88. - С. 127-131.
  2. Ломакина Н. Ф., Гамбарян А. С., Боравлева Е. Ю. и др. Характеристика апатогенного вируса гриппа А/чайка/Москва/3100/2006 (H6N2), выделенного в г. Москве // Молекул. генетика. - 2009. - Т. 29, № 1. - С. 32-40.
  3. Ямникова С. С, Ковтун Т. О., Дмитриев Г. А. и др. Циркуляция вирусов гриппа А серотипа Н13 среди чаек северного Каспия (1979-1985) // Вопр. вирусол. - 1989. - Т. 4. - С. 426-430.
  4. Alexandrova G. I., Polezhaev F. I., Budilovsky G. N. et al. Recombinant cold-adapted attenuated influenza A vaccines for use in children: reactogenicity and antigenic activity of cold-adapted recombinants and analysis of isolates from the vaccines // Infect. and Immun. - 1984. - Vol. 44. - P. 734-739.
  5. Capua I., Alexander D. J. Avian influenza infections in birds-a moving target // Influenza Other Respi --Viruses. 2007. Vol <http://Viruses.-2007.-Vol>. 1, № 1. - P. 11-18.
  6. Desheva J. A., Lu X. H., Rekstin A. R. et al. Characterization of an influenza A H5N2 reassortant as a candidate for live-attenuated and inactivated vaccines against highly pathogenic H5N1 viruses with pandemic potential // --Vaccine. 2006. Vol <http://Vaccine.-2006.-Vol>. 24, № 47-48. - P. 6859-6866.
  7. Ito Т., Goto H., Yamamoto E. et al. Generation of a highly pathogenic avian influenza A virus from an avirulent field isolate by passaging in chickens // J. Virol. - 2001.--- Vol.75. P. 4439 <http://-Vol.75.-P.4439->4443.
  8. Munster V. J., Baas C., Lexmond P. et al. Spatial, temporal, and species variation in prevalence of influenza A viruses in wild migratory birds // PLoS Pathog. - 2007. - Vol. 3, N 5. - P. e61.
  9. Murphy B. R., Sly D. L., Tierney E. L. et al. Reassortant virus derived from avian and human influenza A viruses is attenuated and immunogenic in monkeys// Science. - 1982. - Vol. 218. - P. 1330-1332.
  10. Ovcharenko A. V., Zhirnov O. P. Aprotinin aerosol treatment of influenza and para-myxovirus bronchopneumonia of mice // Antiviral Res. - 1994. - Vol. 23. - P. 107-118.
  11. Richt J. A., Lekcharoensuk P., Lager К. M. et. al. Vaccination of pigs against swine influenza viruses by using an NS1-truncated modified live-virus vaccine // J. Virol. - 2006. - Vol. 80. - P. 11 009-11 018.
  12. Rudneva I. A., Timofeeva T. A., Shilov A. A. et al. Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants // Arch. Virol. - 2007. - Vol. 152. - P. 1139-1145.
  13. Song H., Nieto G. R., Perez D. R. A new generation of modified live-attenuated avian influenza viruses using a two-strategy combination as potential vaccine candidates // J. Virol. - 2007. - Vol. 81. - P. 9238-9248.
  14. Steel J., Lowen A. C., Pena L. et al. Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against H5N1 highly pathogenic avian influenza // J. Virol. - 2009. - Vol.83, N 4. - P. 1742-1753.
  15. Subbarao K., Chen H., Swayne D. et al. Evaluation of a genetically modified reassortant H5N1 influenza A virus vaccine candidate generated by plasmid-based reverse genetics // Virology. - 2003. - Vol. 305. - P. 192-200.
  16. Suguitan A. L. Jr., McAuliffe J., Mills K. L. et al. Live, Attenuated influenza A H5N1 candidate vaccines provide broad cross-protection in mice and ferrets // PLoS Med. - 2006. - Vol. 3, N 9. - P. 1541-1554.
  17. Vincent A. L., Ma W., Lager K. M. et al. Efficacy of intranasal administration of a truncated NS1 modified live influenza virus vaccine in swine // Vaccine. - 2007. - Vol. 25, N 47. - P. 7999-8009.
  18. Wang L., Suarez D. L., Pantin-Jackwood M. et al. Characterization of influenza virus variants with different sizes of the non-structural (NS) genes and their potential as a live influenza vaccine in poultry // Vaccine. - 2008. - Vol. 26, N 29-30. - P. 3580-3586.
  19. Wang L., Yassine H., Saif Y. M., Lee C. W. Developing live attenuated avian influenza virus in ovo vaccines for poultry // Avian Dis. - 2010. - Vol. 54 (Suppl 1). - P. 297-301.
  20. Woolcock P. R., Suarez D. L., Kuney D. Low-pathogenicity avian influenza virus (H6N2) in chickens in California, 2000-02 // Avian Dis. - 2003. - Vol. 47. - P. 872-881.
  21. Yen H.-L., Guan Y., Peiris M., Webster R. G. H5N1 in Asia / Monographs in virology / Eds H. D. Klenk et al. - 2008. - Vo1. 27. - P. 11-13.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2011 Boravleva E.Y., Lomakina N.F., Kropotkina E.A., Rudneva I.A., Yamnikova S.S., Rudenko L.G., Drygin V.V., Gambaryan A.S., Boravleva E.Y., Lomakina N.F., Kropotkina E.A., Rudneva I.A., Yamnikova S.S., Rudenko L.G., Drygin V.V., Gambaryan A.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies