Pathology of lymphoid tissue cells infected by African swine fever virus in vitro


Cite item

Full Text

Abstract

The authors studied the pathology of bone marrow (BM) lymphoid cell from pigs infected by African swine fever virus (ASFV) in vitro. Monocytes were shown to be primarily afflicted in unstimulated BM culture. These cells disappeared completely 72 hours after infection. Just 24 hours following ASFV infection, there were atypical lymphocytes amounting to 12% of the general lymphoid population at hour 72 after inoculation.The area and perimeter of minor, middle, and large lymphocytes tended to reduce during both BM cell cultivation and inoculation. Lymphoblasts and monocytes were generally triploid in both the control and test groups, but among them there were diploid, triploid, and tetraploid cells. Cytophotometric assay revealed that the amount of nuclear DNA significantly increased in BM lymphoblasts and monocytes in the early stages of ASFV infection (within 24 hours). This effect was also rather pronounced in the lymphoblasts in the later stages (at hour 72).

References

  1. Branch D. R., Turner A. R., Guilbert W. Synergistic stimulation of macrophage proliferation by the monokines tumor necrosis factor-alpha and colony-stimulating factor 1 // Blood. - 1989. - Vol. 73. - P. 307.
  2. Chen B. D.-M., Mueller M. Recombinant tumor necrosis factor enhances proliferative responsiveness of murine peripheral macrophages to macrophage colony-stimulating factor but inhibits their proliferative responsiveness to granulocyte-macrophage colony-stimulating factor // Blood. - 1990. - Vol. 75. - P. 1627.
  3. Dixon L. K., Escribano J. M., Martins C. et al. // Virus taxonomy: classification and nomenclature of viruses / Eds C. M. Fauquet et al. - London, 2005. - P. 135-143.
  4. Gil S., Sepulveda N., Albina E. et al. The low-virulent African swine fever virus (ASFV/NH/P68) induces enhanced expression and production of relevant regulatory cytokines (IFNa, TNFa and IL12p40) on porcine macrophages in comparison to the highly virulent ASFV/L60 // Arch. Virol. - 2008. - Vol. 153. - P. 1845-1854.
  5. Gomez M. M., Ortuno E., Fernandez-Zapatero P. et al. African swine fever virus infection induces tumor necrosis factor alpha production: Implications in pathogenesis // J. Virol. - 1999. - Vol. 73, N 3. - P. 2173-2180.
  6. Gomez-Villamandos J. C., Hervas J., Mendez A. et al. Experimental African swine fever: apoptosis of lymphocytes and virus replication in other cells // J. Gen. Virol. - 1995. - Vol. 76. - P. 2399-2405.
  7. Gomez-Villamandos J. C., Bautista M. J., Carrasco L. et al African swine fever virus infection of bone marrow: Lesions and pathogenesis // Vet. Pathol. - 1997. - Vol. 34. - P. 97-107.
  8. Inman D. R., Cooper E. H. The relation of ultrastructure to DNA synthesis in human leukocytes // Acta Haematol. - 1965. - Vol. 33. - P. 257-278.
  9. Mebus C. A. Pathobiology and pathogenesis // African swine fever / Ed. Y. Becker. - Boston, 1987. - P. 21-30.
  10. Okamoto H., Kimura M., Watanabe N., Ogihara M. Tumor necrosis factor (TNF) receptor-2-mediated DNA synthesis and proliferation in primary cultures of adult rat hepatocytes: The involvement of endogenous transforming growth factor-alpha // Eur. J. Pharmacol. - 2009. - Vol. 14, N 1-3. - P. 12-19.
  11. Plowright W., Parker J., Staple R. F. The growth of a virulent strain of African swine fever virus in domestic pigs // J. Hyg. - 1968. - Vol. 66. - P. 117-134.
  12. Rowlands R. J., Michaud V., Heath L. et al. African swine fever virus isolate, Georgia, 2007 // Emerg. Infect. Dis. - 2008. - Vol. 14, N 12. - P. 1870-1874.
  13. Sansone G. A new type of congenital dyserythropoietic anaemia // Br. J. Haematol. - 1978. - Vol. 39. - P. 537.
  14. Simon M. W. The atypical lymphocyte // Int. Pediatr. - 2003. - Vol. 18, N 1. - P. 20-22.
  15. Wardley R. C., Wilkinson P. J., Hamilton F. African swine fever virus replication in porcine lymphocytes // J. Gen. Virol. - 1977. - Vol. 37. - P. 425-427.
  16. Whittall J. T. D., Parkhouse R. M. E. Changes in swine macrophage phenotype after infection with African swine fever virus: cytokine production and responsiveness to interferon-γ and lipopolysaccharide // Immunology. - 1997. - Vol. 91, N 3. - P. 444-449.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2011 Karalova E.M., Voskanyan G.E., Sarkisyan K.V., Abroyan L.O., Avetisyan A.S., Akopyan L.A., Semerdzhyan Z.B., Zakaryan O.S., Arzumanyan G.A., Karalyan Z.A., Karalova E.M., Voskanyan G.E., Sarkisyan K.V., Abroyan L.O., Avetisyan A.S., Akopyan L.A., Semerdzhyan Z.B., Zakaryan O.S., Arzumanyan G.A., Karalyan Z.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies