RT-PCR detection of deformed wing virus in the honey bee Apis mellifera L. in the Moscow Region


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Abstract

Deformed wing virus (DWV) was first detected in the honey bee Apis mellifera by reverse transcriptase-polymerase chain reaction (RT-PCT) in the Moscow Region. Molecular phylogenetic analysis of the detected nucleotide sequence of the virus fragment VP2-VP1 of DWV demonstrated that the Russian virus sequence is united in the common cluster with all earlier revealed nucleotide sequences of DWV in the Genbank worldwide, which confirms the previous conclusions that this virus has recently distributed in the honey bee by Varroa destructor mite. It has been shown that the level of homology for all DWV nucleotide sequences is 98%, except for nucleoside sequence of 7D isolate from Turkey (96% homology), 96% homology with Kakugo virus and 84-86% homology with Varroa destructor virus 1; there is a preponderance of insignificant nucleotide substitutions, mainly transitions, which supports the evolutionary propinquity of 3 viruses.

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Copyright (c) 2010 Udina I.G., Kunizheva S.S., Grishechkin A.E., Kalashnikov A.E., Uchaeva B.C., Krivtsov N.I., Zlobin V.I., Udina I.G., Kunizheva S.S., Grishechkin A.E., Kalashnikov A.E., Uchaeva V.S., Krivtsov N.I., Zlobin V.I.

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