LMP1 gene polymorphism in patients with Epstein-Barr virus-negative gastric carcinomas in Russia


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Abstract

The investigation was undertaken to study the molecular characteristics of Epstein-Barr virus (EBV) LMP1 gene samples amplified from the tumor and intact tissues of patients with EBV-negative forms of gastric carcinoma (GC). The genetic structure of these samples determined by their sequencing was compared with that of the gene samples isolated from the cells of oropharyngeal washing specimens from the same patients with GC, as well as peripheral blood lymphocytes of patients with infectious mononucleosis (IM) and blood donors. The findings suggest that the samples of tumor tissue LMP1 from patients with GC have higher divergence than those from patients with IM and blood donors although no specific variants of the gene for GC were found. Comparison of LMP1 sequences from tumor tissue and cells of oropharyngeal washing specimens from the same patients with EBV-negative GC revealed the common LMP1 variant in 2 cases while they differed in 3 cases. The findings are an initial step in studying the role of EBV in the carcinogenesis of EBV-negative GC that is likely to be established by investigations on representative clinical material, by applying the up-to-date technologies.

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Copyright (c) 2010 Gurtsevich V.E., Smirnova K.V., Diduk S.V., Goncharova E.V., Shcherbak L.N., Senyuta N.B., Kovrigina A.M., Stepina V.N., Nikulin M.P., Yakovleva L.S., Gurtsevich V.E., Smirnova K.V., Diduk S.V., Goncharova E.V., Shcherback L.N., Senyuta N.B., Kovrigina A.M., Stepina V.N., Nikulin M.P., Yakovleva L.S.

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