Molecular genetic characteristics of the Newcastle disease virus velogenic strains isolated in Russia, Ukraine, Kazakhstan, and Kirghizia


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Abstract

The F gene fragment of 79 Newcastle disease virus (NDV) strains isolated from domestic and synanthropic birds in Kazakhstan, Kirghizia, Ukraine, and Russia in 1993 to 2007 was comparatively analyzed. Phylogenetic analysis of test isolates and reference NDV strains obtained from the GenBank was carried out by polymerase chain reaction with subsequent sequencing and comparative analysis of 154-bp nucleotide sequences in the main functional region of the F gene. All newly characterized isolates belong to three NDV genotype VII subgroups: VIIa, VIIb, VIId.
The results show it necessary to monitor of NDV strains isolated in the CIS countries since the spread of NDV among migratory and synanthropic birds (pigeons, crows, and jackdaws) poses a serious threat to commercial poultry industry.

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Copyright (c) 2010 Korotetskiy I.S., Bogoyavlenskiy A.P., Prilipov A.G., Usachev E.V., Usacheva O.V., Turmagambetova A.S., Zaytseva I.A., Kydyrmanov A., Shakhvorostova L.I., Sayatov M.K., Borisov V.V., Pchelkina I.P., Gerilovich A.P., Berezin V.E., Korotetsky I.S., Bogoyavlensky A.P., Prilipov A.G., Usachev E.V., Usacheva O.V., Turmagambetova A.S., Zaitseva I.A., Kydyrmanov A., Shakhvorostova L.I., Sayatov M.K., Borisov V.V., Pchelkina I.P., Gerilovich A.P., Berezin V.E.

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