HERV-K-associated carcinogenesis: co-expression of viral and cellular proteins in the development of human germ-cell tumors


Cite item

Full Text

Abstract

To elucidate the role of some viral and cellular proteins in the occurrence and development of HERV-K-associated germ-cell tumors (GCT), reverse-transcription polymerase chain reaction using specific primers has been employed to study the transcription of the protein Rec HERV-K and the possible interaction of the protein Rec(cORF), that has transforming properties, and the cellular protein PLZF, that is a negative regulator of cell division, in human GCT tissues, in the testicular parenchyma adjacent to a tumor, and in the normal testicular tissues. It was shown that there was expression of Rec(cORF) of mRNA, rather than cellular PLZF in all malignant GCT tissues, this led to the conclusion that no interaction occured between the Rec HERV-K and PLZF proteins in the GCT cells. At the same time co-expression of Rec and PLZF protein was first revealed at the level of transcription in the testicular parenchyma adjacent to a tumor that exhibited carcinoma in situ cells. By taking into account that the protein Rec HERV-K has transforming activity and it is presumed to be implicated in the development of GCT, the authors discuss a possible role in the Rec HERV-K/HTDV and cellular PLZF interaction in the pathogenesis of GST at the early stages of its genesis.

References

  1. Клейман А. М., Сенюта Н. Б., Трякин А. А. и др. Экспрессия эндогенных ретровирусов семейства HERV-K/HTDV у больных герминогенными опухолями яичка // Вопр. вирусол. - 2001. - № 6. - С. 15-21.
  2. Лебедев Ю. Б. Эндогенные ретровирусы: возможная роль в функционировании клеток человека // Молекул. биол. - 2000. - Т. 34, № 4. - С. 635-645.
  3. Barbulescu M., Turner G., Seaman M. I. et al. Many human endogenous retrovirus K (HERV-K) proviruses are unique to humans // Curr. Biol. - 1999. - Vol. 9. - P. 861-969.
  4. Boese A., Sauter M., Galli U. et al. Human endogenous retrovirus protein cORF supports cell transformation and associated with the promyelocytic leukemia zinc finger protein // Oncogene. - 2000. - Vol. 19. - P. 4328-4336.
  5. Buscher K., Trefzer U., Hofman M. et al. Expression of human endogenous retrovirus K in melanomas and melanoma cell lines // Cancer Res. - 2005. - Vol. 65. - P. 4172-4180.
  6. Denne M., Sauter M., Armbruester V. et al. Physical and functional interactions of human endogenous retrovirus proteins Np9 and Rec with the promyelocytic leukemia zinc finger protein // J. Virol. - 2007. - Vol. 81. - P. 5607-5616.
  7. Depil S., Roche C., Dussart P., Prin I. Expression of human endogenous retrovirus, HERV-K, in the blood cells of leukemia patients // Leukemia. - 2002. - Vol. 16. - P. 254-259.
  8. Galli U., Sauter M., Lecher B. et al. Human endogenous retrovirus rec interferes with germ cell development in mice and may cause carcinoma in situ, the predecessor lesion of germ cell tumors // Oncogene. - 2005. - Vol. 24. - P. 3223-3228.
  9. Herbst H., Sauter M., Muller-Lantzsh N. et al. Expression of human endogenous retrovirus K element in germ cell and trophoblastic tumors // Am. J. Pathol. - 1996. - Vol. 149. - P. 1727-1735.
  10. Kleiman A., Senyuta N.,Tryakin A. et al. HERV-K(HML-2) Gag/Env antibodies as indicator for therapy effect in patients with germ cell tumor // Int. J. Cancer. - 2004. - Vol. 110. - P. 459-461.
  11. Lander E. S., Linton L. M., Birren B. et al. Initial sequencing and analysis of the human genome // Nature. - 2001. - Vol. 409. - P. 860-921.
  12. Lower R., Boller K., Hasenmaier B. et al. Identification of human endogenous retroviruses with complex mRNA expression and particle formation // Proc. Natl. Acad. Sci. USA. - 1993. - Vol. 90. - P. 4480-4484.
  13. Lower R., Tonjes R., Korbmacher C. et al. Identification of a Rev related protein by analysis of spliced transcripts of the human endogenous retroviruses HTDV/HERV-K // J. Virol. - 1995. - Vol. 69. - P. 141-149.
  14. Magin C., Lower R., Lower J. cORF and RcRe, the Rev/Rex and RRE/RxRe homologues of the human endogenous retrovirus family HTDV/HERV-K // J. Virol. - 1999. - Vol. 73. - P. 9496-9507.
  15. Mayer J., Sauter M., Racz A. et al. An almost intact human endogenous retrovirus K on human chromosome 7// Nat. Genet. - 1999. - Vol. 21. - P. 257-258.
  16. Mi S., Lee X., Li X. et al. Syncytin is a captive retroviral envelope protein involved in human placental morphogenesis // Nature. - 2000. - Vol. 403. - P. 785-789.
  17. Mosselman S., Looijenga H. J., Gillis A. S. M. et al. Aberrant platelet-derived growth factor-receptor transcript as a diagnostic marker for early human germ cell tumors of the adult testis // Proc. Natl. Acad. Sci. USA. - 1996. - Vol. 93. - P. 2884-2888.
  18. Ono M., Yasunaga T., Miyata T. Nucleotide sequence of human endogenous retrovirus genome related to the miuse mammary tumor virus genome // J. Virol. - 1986. - Vol. 60. - P. 589-598.
  19. Ravnik S. E., Wolgemuth D. J. Regulation of meiosis during mammalian spermatogenesis: the A-type cyclins and their associated cyclin-dependent kinases are differentially expressed in the germ cell lineage // Dev. Biol. - 1998. - Vol. 207. - P. 408-418.
  20. Sauter M., Roemer K., Best B. et al. Specificity of antibodies directed against env protein of human endogenous retroviruses in patients with germ cell tumors // Cancer Res. - 1996. - Vol.56. - P. 4362-4365.
  21. Wang-Johanning F., Frost A. R., Jian B. et al. Quantitation of HERV-K env gene expression and splicing in human brest cancer // Oncogene. - 2003. - Vol. 22. - P. 1528-1535.
  22. Zhang T., Xiong H., Kan L.-X. et al. Genomic sequence, structural organization, molecular evolution and aberrant rearrangement of promyelocytic leukemia zink finger gene // Proc. Natl. Acad. Sci. USA. - 1999. - Vol. 96. - P. 11422-11427.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2009 Senyuta N.B., Kleyman A.M., Karseladze A.I., Tryakin A.A., Goncharova E.V., Tyulyandin S.A., Gurtsevich V.E., Senyuta N.B., Kleiman A.M., Karseladze A.I., Tryakin A.A., Goncharova Y.V., Tyulyandin S.A., Gurtsevich V.E.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies