Differential incorporation of genomic segments into the influenza A virus reassortants in mixed infection


Cite item

Full Text

Abstract

The gene composition of the viral population obtained via mixed infection of embryonated chick eggs with influenza viruses at a high multiplicity of infection was analyzed. In mixed infection caused by influenza A/WSN/33 (H1N1) and A/Duck/Czechoslovakia/56 (H4N6) viruses, the population showed a preponderance of the reassortants whose content of genomic segments originating from either of the parent virus deviated strongly from the random pattern: the hemagglutinin (HA) gene of A/WSN/33 (H1N1) virus and the NP gene of A/Duck/Czechoslovakia/56 (H4N6) virus were prevalent in the gene composition of the reassortants. The mixed infection produced by influenza A/Udorn/72 (H3N2) virus and the reassortant R8 containing the HA gene of A/Duck/Ho Chi Minh/014/78 (H5N3) virus, the population of reassortants contained mainly the HA gene of A/Udorn/72 (H3N2) virus and the NP gene of the reassortant R8. The findings are discussed due to the problem of specific recognition of gene segments when incorporated into the viral particles.

References

  1. Cox N. J., Maassab H. F., Kendal A. P. Comparative studies of wild-type and cold-adapted (temperature-sensitive) influenza viruses: nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25 degrees between recent H3N2 an H1N1 epidemic strains and cold-adapted A/An Arbor/6/60 // Virology. - 1979. - Vol. 97. - P. 190-194.
  2. Duhaut S. D., McCauley J. W. Defective RNAs inhibit the assembly of influenza virus genome segments in a segment-specific manner // Virology. - 1996. - Vol. 216. - P. 326-337.
  3. Enami M., Sharma G., Benham C., Palese P. An influenza virus containing nine different RNA segments // Virology. - 1991. - Vol. 185. - P. 291-298.
  4. Fujii I., Goto H., Watanabe T. et al. Selective incorporation of influenza virus RNA segments into virions // Proc. Natl. Acad. Sci. USA. - 2003. - Vol. 100. - P. 2002-2007.
  5. Gorman O. T., Bean W. J., Kawaoka Y., Webster R. G. Evolution of the nucleoprotein gene of influenza A virus // J. Virol. - 1990. - Vol. 64. - P. 1487-1497.
  6. Ilyushina N. A., Rudneva I. A., Shilov A. A. et al. Postreassortment changes in a model system: HA-NA adjustment in an H3N2 avian-human reassortant influenza virus // Arch. Virol. - 2005. - Vol. 150. - P. 1327-1338.
  7. Kaverin N. V., Rudneva I. A., Smirnov Y. A., Finskaya N. N. Human-avian influenza virus reassortants: effect of reassortment pattern on multicycle reproduction in MDCK cells // Arch. Virol. - 1988. - Vol. 103. - P. 117-126.
  8. Kaverin N. V., Gambaryan A. S., Bovin N. V. et al. Postreassortment changes in influenza A virus hemagglutinin restoring HA-NA functional match // Virology. - 1998. - Vol. 244. - P. 315-321.
  9. Kaverin N. V., Matrosovich M. N., Gambaryan A. S. et al. Intergenic HA-NA interactions in influenza A virus: postreassortment substitutions of charged amino acid in the hemagglutinin of different subtypes // Virus Res. - 2000. - Vol. 66. - P. 123-129.
  10. Liang Y., Hong Y., Parslow T. G. cis-Acting packaging signals in the influenza virus PB1, PB2, and PA genomic RNA segments // J. Virol. - 2005. - Vol. 79. - P. 10348-10355.
  11. Lubeck M. D., Palese P., Schulman J. L. Nonrandom association of parental genes in influenza A virus recombinants // Virology. - 1979. - Vol. 95. - P. 269-274.
  12. Nakajima K., Sugiura A. Three-factor cross of influenza virus // Virology. - 1977. - Vol. 81. - P. 486-489.
  13. Noronha-Blob L., Schulze I. T. Nonreciprocal and host-dependent interference between two variants of influenza virus // Virology. - 1976. - Vol. 70. - P. 509-516.
  14. Roberts P. C., Lamb R. A., Compans R. W. The M1 and M2 proteins of influenza A virus are important determinants in filamentous particle formation // Virology. - 1998. - Vol. 240. - P. 127-137.
  15. Scholtissek C., Stech J., Krauss S., Webster R. G. Cooperation between the hemagglutinin of avian viruses and the matrix protein of human influenza A viruses // J. Virol. - 2002. - Vol. 76. - P. 1781-1786.
  16. Tobita K. Genetic recombination between influenza virus A0/NWS and A2/Hong Kong // Arch. Ges. Virusforsch. - 1971. - Vol. 34. - P. 119-130.
  17. Varich N. L., Kaverin N. V. Antigenically relevant amino acid positions as revealed by reactions of monoclonal antibodies with the nucleoproteins of closely related influenza A virus strains // Arch. Virol. - 2004. - Vol. 149. - P. 2271-2276.
  18. Webster R. G., Campbell C. H., Granoff A. The in vivo production of new influenza A viruses // Virology. - 1971. - Vol. 44. - P. 317-328.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2009 Varich N.L., Gitel'man A.K., Shilov A.A., Smirnov Y.A., Kaverin N.V., Varich N.L., Gitelman A.K., Shilov A.A., Smirnov Y.A., Kaverin N.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies