Theage-related specificity of site-oriented immunity inrespiratory-cyncytial virus infection


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Abstract

ELISA test systems were designed on the basis of synthetic peptides (SP) simulating the primary structure of functionally
significant epitopes of the respiratory and cy ncyntial virus (RCV) F-protein for the purpose of investigating
the structure and age-related peculiarities of humoral immunity in respect to separate epitopes of PCV F-protein.
One of them (221-232) simulates a part of RCV "virus-neutralizing domain" and another one (479-491) is highly
important for the fusion mechanisms. New SP-based ELISA were used to examine pair sera in 159 patients with
documented RCV infection including children, aged up to 3 years and 3 to 15, and adults. The activity of anti-RCV
antibodies to SP was found to be significantly lower in children aged up to 3 years versus the older children and
especially versus the adults. The virus neutralizing and, to a greater extent, fusion-inhibiting activities of antibodies
were increasing with age, which collated with the results of detecting the antibodies to SP by immuneenzyme
assay. The results testify to synchronism of formation of antibodies to different epitopes of the RCV F-protein.
The shaping-up of antibodies with the above SP could denote the protective properties of humoral immunity,
which justifies the use of the SP-based ELISA in its analysis, especially, in babies as well as in different-type immunodeficiency
and immunopathology conditions.

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Copyright (c) 2004 Krivitskaya V.Z., Sominina A.A., Vlasov G.P., Sukhovetskaya V.F., Golovanova A.K.

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