Identification of bovine diarrhea virus. Genotyping and phylogenetic analysis of isolates obtained in the territory of the Russian Federation


Cite item

Full Text

Abstract

Bovine diarrhea virus (BDV) is widespread throughout the European part of the Russian Federation. The BDV genome is detected in around 40% of animals (aged up to 18 months) with lesions in the gastrointestinal tract and respiratory system. It can be found in all intestinal sections, including its contents and feces, in lymphoid and respiratory organs as well as in nasal washings and discharges and in vaginal tunic discharges of cows. Primary structure analysis of 5'-untransalted region of the genome of the detected isolates revealed that all of them belonged to genotype 1. Phylogenetic analysis confirmed that a part of isolates belonged to subgenotype 1a, while most isolates make up a separate group. No geographic differences were reported in the circulation of the BDV genetic variants within Russia's territory.

References

  1. Вечеров А. Е., Дороненкова Г. Н., Лисицын В. В. и др. Применение ПЦР и нуклеотидного секвенирования гена HN для выявления и штаммовой дифференциации вируса парагриппа-3 КРС //Достижения молодых ученых - в ветеринарной практике: Материалы конф. молодых ученых. - Владимир, 2000. - С. 207-212.
  2. Грибанов О. Г., Щербаков А. В., Перевозчикова Н. А. и др. Простой метод выделения и очистки РНК // Биоорган, химия. - 1997. - Т.-23, № 9. - С. 763-765.
  3. Кудрявцев В. А., Дороненкова Г. Н., Аянот П. К., Гусев А. А. ПЦР и секвенирование вариабельной области гемагглютининэстеразного гена коронавируса КРС // Вестн. РАСХН. - 2002. - № 4. - С. 341-344.
  4. Чупин С. А., Дороненкова Г. Н., Прохватилова Л. Б., Аянот П. К. Использование ПЦР и нуклеотидного секвенирования для дифференциации G-серотипов ротавирусов КРС // Достижения молодых ученых - в ветеринарной практике: Материалы конф. молодых ученых. - Владимир, 2000. -С. 184-190.
  5. Baker J. С. Bovine viral diarrhea virus: a review // J. Am. Vet. Med. Assoc. - 1987. - Vol. 190, N 11. - P. 1449-1458.
  6. Bolin S. R., McClurkin A. W., Cutlip R. C., Coria M. F. Severe clinical disease induced in cattle persistently infected with noncytopathic bovine viral diarrhea virus by superinfection with cytopathic bovine viral diarrhea virus // Am. J. Vet. Res. - 1985. - Vol. 46. - P. 573-576.
  7. Bolin S. R., Littledike E. Т., Ridpath J. F. Serologic detection and practical consequences of antigenic diversity among bovine viral diarrhea viruses in a vaccinated herd // Ibid. - 1991. - Vol. 52. - P. 1033-1037.
  8. Boye M., Kamstrup S., Dalsgaard K. Specific sequence amplification of bovine viral diarrhea virus (BVDV) and hog cholera virus and sequencing of BVDV nucleic acid // Vet. Microbiol. - 1991. -Vol. 29. - P. 1-13.
  9. Brownlie J., Clarke M. C., Howard С. J. Experimental production of fatal mucosal disease in cattle // Vet. Rec. - 1984. - Vol. 114. - P. 535-536.
  10. Collet M. S., Larson R., Gold С. et al. Molecular choning and nucleotide sequence of the pestivirus bovine viral diarrhea virus // Virolody. - 1988. - Vol. 165, N I. - P. 191-199.
  11. Corapi W. M., Elliott R. D., French T. W. et al. Thrombocytopenia and hemorrhager in veal calves infected with bovine viral diarrhea virus // J. Am. Vet. Med. Assoc. - 1990. - Vol. 196, N 4. - P. 590-596.
  12. David G. P., Crawshaw T. R., Gunning R. F. Sever disease in adult dairy cattle in three UK dairy herds associated with bovine viral diarrhea virus infection // Vet. Rec. - 1994. - Vol. 134, - P. 468-472.
  13. Katz J. В., Ridpath J. F., Bolin S. R. Presumptive diagnostic differentiation of hog cholera virus from bovine diarrhea and border disease viruses by using a cDNA nestedamplification approach // J. Clin. Microbiol. - 1993. - Vol. 31. - P. 565568.
  14. Murphy F. A., Fauquet С. M., Bishop D. H. L. // Virus taxonomy - the classification and nomenclature of viruses: 6-th Rep. Int. Committee on Taxonomy of Viruses. - Vienna, 1995.
  15. Pellerin C., van den Hurk J., Lecomte J., Tijssen P. Identification of a new group of bovine virel diarrhea virus strains associated with severe outbreaks and high mortalities // Virology. - 1994. - Vol. 203, N 2. - P. 260-268.
  16. Pellerin C., Moir S., Lecomte J., Tijssen P. Comparison of the pi 25 coding region of bovine viral diarrhea virus // Vet. Microbiol. - 1995. - Vol. 45. - P. 45-57.
  17. Radostits О. М., Littlejohns I. R. New concepts in the pathogenesis diagnosis and control of disease caused by the bovine viral diarrhea virus // Can. Vet. J. - 1988. - Vol. 29. - P. 513528.
  18. Ridpath J., Bolin S., Dubovi E. Segregation of bovine viral diarrhea virus into genotypes // Virology. - 1994. - Vol. 205, N 1. - P. 66-74.
  19. Sanger F., Nicklen S., Coulsen A. R. DNA sequencing with chain terminating inhibitors // Proc. Natl. Acad. Sci. USA. - 1977. - Vol. 74. - P. 5463-5467.

Supplementary files

Supplementary Files
Action
1. JATS XML

Copyright (c) 2003 Schulpin M.I., Ayanot P.K., Mischenko V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС77-77676 от 29.01.2020.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies