Evaluation of the human sensitivity to smallpox virus by the primary cultures of the monocyte-macrophages

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Abstract

Studies of the primary cultures of granulocytes, mononuclear, and monocyte-macrophage cells derived from human blood were performed using variola virus (VARV) in the doses of 0.001-0.021 PFU/cell (plaques-forming units per cell). Positive dynamics of the virus accumulation was observed only in the monocyte-macrophages with maximum values of virus concentration (5.0-5.5 lg PFU/ml) mainly within six days after the infection. The fact of VARV replication in the monocyte-macrophages was confirmed by the data of electron microscopy. At the same time, virus vaccines when tested in doses 3.3 and 4.2 lg PFU/ml did not show the ability to reproduce in these human cells. The people sensitivity to VARV as assessed from the data obtained on human monocytemacrophages corresponded to ~1 PFU (taking into account the smooth interaction of the virus in the body to the cells of this type), which is consistent to previously found theoretical data on the virus sensitivity. The human susceptibility to VARV assessed experimentally can be used to predict the adequacy of developed smallpox models (in vivo) based on susceptible animals. This is necessary for reliable assessment of the efficiency of development of drugs for treatment and prophylaxis of the smallpox.

About the authors

A. S. Zamedyanskaya

State Research Center for Virology and Biotechnology «Vector»

Author for correspondence.
Email: zamedyanskaya_as@vector.nsc.ru
ORCID iD: 0000-0002-1177-2693

Alena S. Zamedyanskaya, Junior research scientist

Koltsovo, Novosibirsk region, 630559

Russian Federation

K. A. Titova

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0001-8764-9408
Koltsovo, Novosibirsk region, 630559 Russian Federation

Al. A. Sergeev

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0001-8355-5551
Koltsovo, Novosibirsk region, 630559 Russian Federation

A. S. Kabanov

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-6287-0912
Koltsovo, Novosibirsk region, 630559 Russian Federation

L. E. Bulychev

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0001-9598-7327
Koltsovo, Novosibirsk region, 630559 Russian Federation

Ar. A. Sergeev

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-3591-1571
Koltsovo, Novosibirsk region, 630559 Russian Federation

D. O. Galakhova

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-7801-4141
Koltsovo, Novosibirsk region, 630559 Russian Federation

A. E. Nesterov

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0001-7943-3287
Koltsovo, Novosibirsk region, 630559 Russian Federation

O. V. Nosareva

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0003-2387-4299
Koltsovo, Novosibirsk region, 630559 Russian Federation

L. N. Shishkina

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-8264-0217
Koltsovo, Novosibirsk region, 630559 Russian Federation

O. S. Taranov

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-6746-8092
Koltsovo, Novosibirsk region, 630559 Russian Federation

V. V. Omigov

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0002-2028-6099
Koltsovo, Novosibirsk region, 630559 Russian Federation

A. P. Agafonov

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0003-2577-0434
Koltsovo, Novosibirsk region, 630559 Russian Federation

A. N. Sergeev

State Research Center for Virology and Biotechnology «Vector»

Email: fake@neicon.ru
ORCID iD: 0000-0001-5984-8776
Koltsovo, Novosibirsk region, 630559 Russian Federation

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Copyright (c) 2016 Zamedyanskaya A.S., Titova K.A., Sergeev A.A., Kabanov A.S., Bulychev L.E., Sergeev A.A., Galakhova D.O., Nesterov A.E., Nosareva O.V., Shishkina L.N., Taranov O.S., Omigov V.V., Agafonov A.P., Sergeev A.N.

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