DIMERIC BISBENZIMIDAZOLES SUPPRESS THE HERPES SIMPLEX VIRUS AND HUMAN CYTOMEGALOVIRUS INFECTIONS IN CELL СULTURES

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Abstract

Antiviral activity of new AТ-specific fluorescent symmetric dimeric bisbenzimidazoles of DBА(n) series was assessed in the cell models of infections caused by type 1 herpes simplex virus (HSV1) and human cytomegalovirus (CMV). In DBA(n) molecules bisbenzimidazole fragments are bound to an oligomethylene liner with varied number of methylene groups in the linker (n = 1, 3, 5, 7, 9, 11). In contrast to DB(n) dimeric bisbenzimidazoles, in DBA(n) series terminal fragments of macromolecules contain N-dimethylaminopropylcarboxamide groups instead of N-methylpiperazine groups. DBА(n) compounds better dissolve in water, pass across plasma and nuclear membrane, and stain DNA in living cells. DBA(1) and DBA(7) produced therapeutic effects in HSV1 infection; DBA(7) completely suppressed the infection. DBA(11) displayed in vitro therapeutic activity in HSV1 and CMV infections. In addition, DBA(7) and DBA(1) showed microbicidal activity. Thus, DBA(11), which is active against two viruses causing severe diseases with serious health consequences for immunodeficient individuals, should be further investigated. High antiviral activity of DBA(7) in all test models indicates that this compound is a promising active agent for innovative antiviral drugs.

About the authors

R. R. Klimova

D.I. Ivanovsky Institute of Virology «Federal Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»

Author for correspondence.
Email: regi.k@mail.ru
Russian Federation

E. D. Momotyuk

D.I. Ivanovsky Institute of Virology «Federal Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»; Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

Email: noemail@neicon.ru
Russian Federation

N. A. Demidova

Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

Email: noemail@neicon.ru
Russian Federation

Ya. Yu. Chernoryzh

D.I. Ivanovsky Institute of Virology «Federal Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»

Email: noemail@neicon.ru
Russian Federation

V. S. Koval

Engelhardt Institute of Molecular Biology; Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry

Email: noemail@neicon.ru
Russian Federation

A. A. Ivanov

Blokhin Russian Cancer Research Center, Research Institute of Carcinogenesis

Email: noemail@neicon.ru
Russian Federation

A. L. Zhuze

Engelhardt Institute of Molecular Biology

Email: noemail@neicon.ru
Russian Federation

A. A. Kushch

D.I. Ivanovsky Institute of Virology «Federal Research Center of Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya»

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2017 Klimova R.R., Momotyuk E.D., Demidova N.A., Chernoryzh Y.Y., Koval V.S., Ivanov A.A., Zhuze A.L., Kushch A.A.

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