Development of ELISA on the Basis of Monoclonal Antibodies for Detecting Specific Activity of the Vaccine against Hemorrhagic Fever with Renal Syndrome


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Abstract

The monoclonal antibodies to Puumala, Dobrava, Hantaan, and Seoul hantaviruses were obtained using mice. The viruses were known to cause HFRS, and two variants of ELISA were designed. First, Hanta-PUU variant, was constructed using monoclonal antibodies to Puumala virus envelope glycoprotein (G N:G C) for detecting only Puumala virus antigen. The second, Hanta-N variant, was constructed using monoclonal antibodies to Dobrava and Puumala nucleocapsid proteins for detecting four above mentioned hantaviruses. Both Hanta-PUU and Hanta-N assays were reliable in detecting specific hantavirus antigens and the immunogenecity of hantavirus vaccines.

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Разработка иммуноферментной тест-системы на основе моноклональных антител для определения специфической активности вакцины против геморрагической лихорадки с почечным синдромом
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References

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Copyright (c) 2013 Dzagurova T.K., Solopova O.N., Sveshnikov P.G., Korotina N.A., Balovneva M.V., Leonovich O.A., Varlamov N.E., Malkin G.A., Sotskova S.E., Tkachenko E.A.

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