Evaluation of the efficiency and reactogenicity of emulsion-based adjuvant systems in the manufacture of polyclonal enteroviral diagnostic sera


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Abstract

Whether various adjuvants might be used in the manufacture of commercial enteroviral diagnostic sera (EDS) was studied. The following adjuvants: Ribi, SAF-1, and TiterMax were compared; vaseline-lanoline emulsion used to prepare EDSs, as well as modified Freund's complete adjuvant served as controls. Chinchilla rabbits were intramuscularly injected enterovirus antigens (enterovirus 70 and ECHO 2) together with the adjuvant emulsions. TiterMax showed the highest efficiency comparable with the activity of Freund's adjuvant. The activities of Ribi, SAF-1, and vaseline-lanoline emulsion were 3-4 times lower. The neutralizing activity of the sera obtained after 2-3 (TiterMax) or 4-5 (Ribi, SAF-1) immunizations was maximal. Further immunizations resulted in a reduction in the titers of neutralizing antibodies. TiterMax and vaseline-lanoline emulsion caused minimal complications at the site of inoculation whereas SAF-1 and Ribi gave rise to severer inflammatory responses.

References

  1. Кожевникова Т. Н., Ворович М. Ф., Козлов В. Г. и др. Использование фоспренила при изготовлении гипериммунных сывороток // Рос. вет. журн. - 2006. - № 2. - С. 8-10.
  2. Козлов В. Г., Ожерелков С. В., Иванова О. Е., Грачев В. П. Получение кроличьих гипериммунных энтеровирусных сывороток с помощью иммуномодуляторов различной природы // Медицинская вирусология: Сборник. - М., 2006. - Т. 22. - С. 49-53.
  3. Козлов В. Г., Ожерелков С. В. Применение иммуномодуляторов при изготовлении поликлональных энтеровирусных диагностических сывороток // Вакцинология 2008. Совершенствование иммунобиологических средств профилактики, диагностики и лечения инфекционных болезней: Сборник. - М., 2008. - С. 63-64.
  4. Кравченко А. Т., Цетлин Е. М., Омельченко Т. Н. Рациональный метод получения сывороток с высокими титрами вируснейтрализующих антител. Сообщение III // Журн. микробиол. - 1980. - № 7. - С. 50-54.
  5. Рекомендации по эпидемиологическому надзору за энтеровирусами для поддержки программы ликвидации полиомиелита. - Женева, ВОЗ, 2005.
  6. Руководство по лабораторным исследованиям полиомиелита. - 4-е изд. - Женева, ВОЗ, 2005.
  7. Alkira S. Toll-like receptor signaling // J. Biol. Chem. - 2003. - Vol. 278. - P. 38105-38108.
  8. Allison A. C. Squalene and squalane emulsions as adjuvants // Methods. - 1999. - Vol. 9. - P. 87-93.
  9. Bennett B., Check I., Olsen M., Hunter R. A comparison of commercially available adjuvants for use in research // J. Immunol. Meth. - 1992. - Vol. 153. - P. 31-40.
  10. Biars N. E., Allison A. S. Sintex adjuvant formulation // The theory and practical application of adjuvants / Ed. D. E. S. Stewart-Tull. - New York, 1995. - P. 51-94.
  11. Bollen L., Crowley A., Stodulski G., Hau J. Antibody production in rabbits and chickens immunized with human IgG. A comparison of titre and avidity development in rabbit serum, chicken serum and egg yolk using three different adjuvants // J. Immunol. Meth. - 1996. - Vol. 191. - P. 113-120.
  12. Broderson J. R. A retrospective review of lessons associated with the use of Freund's adjuvant // Lab. Anim. Sci. - 1989. - Vol. 39. - P. 400-405.
  13. Byars N. E., Allison A. C. Adjuvant formulation for use in vaccines to elicit both cell-mediated and humoral immunity // Vaccine. - 1987. - Vol. 5. - P. 223-228.
  14. CCAC (Canadian Council on Animal Care). CCAC Guidelines on antibody production. - Ottawa, 2002.
  15. Cox J., Coulter A. Adjuvants - a classification and review of the modes of action // Vaccine. - 1997. - Vol. 15. - P. 248-256.
  16. Dalpke A., Heeg K. Signal integration following toll-like receptor triggering // Crit. Rev. Immunol. - 2002. - Vol. 22. - P. 217-250.
  17. Deeb B., DiGiacomo R., Kunz L., Stewart J. Comparison of Freund's and Ribi adjuvants for inducing antibodies to synthetic antigen (TG)-AL in rabbits // J. Immunol. Meth. - 1992. - Vol. 152. - P. 105-113.
  18. Hanly W., Artwohl J., Bennett B. Adjuvant and antibody production: Review of polyclonal antibody production procedures in mammals and poultry //. ILAR J. - 1995. - Vol. 37, N 3.
  19. Hunter R. L., Bennett B. The adjuvant activity of nonionic block polimer surfactants: III. Characterization of selected biologically active surfaces // Scand. J. Immunol. - 1986. - Vol. 23. - P. 287-300.
  20. Hunter R. L., Olsen V., Bennett B. Copolymer adjuvants and TiterMax // The theory and practical application of adjuvants / Ed. D. E. S. Stewart-Tull. - New York, 1995. - P. 51-94.
  21. Janeway C. A., Medzhitov R. Innate immune recognition // Annu. Rev. Vaccines. - 2002. - Vol. 20. - P. 197-216.
  22. Jennings V. Adjuvants and antibody production: Review of selected adjuvants used in antibody production // ILAR J. - 1995. - Vol. 37, N 3.
  23. Johnston B. A., Eisen H., Fry D. An evaluation of several adjuvant emulsion regimens for the production of polyclonal antisera in rabbits // Lab. Anim. Sci. - 1991. - Vol. 41. - P. 15-21.
  24. Kawai T., Akira S. TLR signaling // Semin. Immunol. - 2007. - Vol. 19. - P. 24-32.
  25. Kono H., Rock K. How dying cells alert the immune system to danger // Nat. Rev. Immunol. - 2008. - Vol. 8. - P. 279-289.
  26. Kotany S., Takada I., Takahashi T. et al. Immunobiological activities of synthetic lipid A analogs with low endotoxicity // Infect. Immun. - 1986. - Vol. 54. - P. 673-682.
  27. Leenaars P., Koedam M., Wester P. et al. Assessment of side effects induced by infection of different adjuvant/antigen combinations in rabbits and mice // Lab. Anim. (New York). - 1998. - Vol. 32. - P. 387-406.
  28. Lipman N. S., Trudel L., Murphy J., Sahali Y. Comparison of immune response potentiation and in vivo inflammatory effects of Freund's and Ribi adjuvants in mice // Lab. Anim. Sci. - 1992. - Vol. 42. - P. 193-197.
  29. Mallon F. M., Graichen M. E., Conway B. R. et al. Comparison of antibody response by the use of synthetic adjuvant system and Freund complete adjuvant in rabbits // Am. J. Vet. Res. - 1991. - Vol. 52, N 9. - P. 1505-1506.
  30. Pallansch M. A., Roos R. P. Enteroviruses: polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses // Fields virology / Eds D. M. Knipe et al. - Philadelphia, 2001. - P. 723-775.
  31. Powers J., Naash P., Rhyan J. et al. Comparison of immune and adverse effects induced by AdjuVac and Freund's complete adjuvant in New Zealand white rabbits (Oryctolagus cuniculus) // Lab. Anim. (New York). - 2007. - Vol. 36, N 9. - P. 51-58.
  32. Quesniaux V. J., Nicolle D., Torres D. et al. Toll-like receptor 2 (TLR2)-dependent-positive and TLR2-independent-negative regulation of proinflammatory cytokines by micobacterial lipomannans // J Immunol. - 2004. - Vol. 172. - P. 4425-4434.
  33. Reed S., Bertholet S., Coler R., Friede M. New horizons in adjuvants for vaccine development // Trends Immunol. - 2008. - Vol. 30, N 1. - P. 23-32.
  34. Robucco J. A., Griffith J. W., Chroscinski E. A. et al. Comparison of the effects of five adjuvants on the antibody response to influenza virus antigen in guinea pigs // Lab. Anim. Sci. - 1995. - Vol. 45. - P. 420-425.
  35. Rudbach J. A., Johnston D., Ulrich J. Ribi adjuvants: Chemistry, biology and utility in vaccines for human and veterinary medicine // The theory and practical application of adjuvants / Ed. D. E. S. Stewart-Tull. - New York, 1995. - P. 287-313.
  36. Smith D. E., O'Brien M., Palmer V., Sadowski J. The selection of an adjuvant emulsion for polyclonal antibody production using of low-molecular-weight antigen in rabbits // Lab. Anim. Sci. - 1992. - Vol. 42. - P. 599-601.
  37. Stewart-Tull D. Freund-type mineral oil adjuvant emulsions // The theory and practical application of adjuvants / Ed. D. Stewart-Tull. - New York, 1995. - P. 1-19.
  38. Stewart-Tull D. Harmful and beneficial activities of immunological adjuvants // Methods in molecular medicine: Vaccine adjuvants - preparation methods and research protocols / Ed. D. O'Hagan. - Totowa, New Jersey, 2000. - P. 29-48.
  39. Stills H. Adjuvants and antibody production: Dispelling the myths associated with Freund's complete and other adjuvants // ILAR J. - 2005. - Vol. 46. - P. 280-293.
  40. Takeuchi O., Akira S. Recognition of viruses by innate immunity // Immunol. Rev. - 2007. - Vol. 220. - P. 214-224.
  41. Vogel F., Powell M. A compendium of vaccine adjuvants and excipients // Vaccine design: The subunit and adjuvant approach / Eds M. Powell, M. Newmann. - New York, 1995. - P. 141-227.
  42. Yamanaka M., Hiramatsu K., Hirahara T. et al. Pathological studies on local tissue reactions in guinea pigs and rats caused by four different adjuvants // J. Vet. Med. Sci. - 1992. - Vol. 54. - P. 685-691.

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