Study of the functional role of mutation in the guinea pig-adapted Ebola virus genome on a Drosophila melanogaster model


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Abstract

Ebola virus virulence in guinea pigs, which appears through virus adaptation to this animal host, correlates with substitutions in the gene encoding vp24 protein. In particular, the substitution His → Tyr186 was found when obtaining strain 8 ms. An attempt was made to clarify the functional role of this substitution in a transgenic fruit fly model. Using the drosophila transformation technique provided transgenic strains that contained genomic insertions of wild-type Ebola virus vp24 gene and the mutant gene with the His → Tyr substitution at the above position. Thus, the drosophila strains carrying the sequences encoding for the vp24 proteins of Ebola virus Zaire and 8 ms in pUAST vector were obtained. This makes it possible to study the expression of transgenic constructs in various D. melanogaster organs and tissues.

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Copyright (c) 2011 Shelemba-Chepurnova A.A., Omel'yanchuk L.V., Chepurnov A.A., Shelemba-Chepurnova A.A., Omelyanchuk L.V., Chepurnov A.A.

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